FastQCFastQC Report
Tue 10 May 2022
EGAF00003633435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003633435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15532
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTCGCATACCGGTAGGCGCGCCGCAGCGCCATCGCCACTTCATGAAA360.23177955189286636No Hit
GCGCCAGCGAAACGCTGGCGCTCAGCTTGTCGTGCTTGCCGAACCAGCCG310.19958794746330155No Hit
CTTTTGTAGAATCATAAGATGCCTTTGATTCTAAGTATTTTTTTCCTCTT300.19314962657738863No Hit
GCCAACGCTTGTCCCTATGCGCCTGATTACGCTGTTCCTGCTGCTGTTGC280.18027298480556272No Hit
ATCAAAAACGATGGCAGGTTGAAGTGTATCATAAGTCGTTAAAGCAAAAT240.1545197012619109No Hit
AGAGATTTTTGACTGCACAAAAGCTGACTTCGGTCAGCTTTTTTATTGGC230.14808138037599794No Hit
CGCCTACCTCGTGCAGACCGCGGAGAAGGGGCTCGCCTGGCTCAACCTCA210.13520473860417204No Hit
TAGTGACACTGGGTTTAACCACCTATGTAGTCGAGTTCGCACCCCAACTT210.13520473860417204No Hit
TGCAAGACATGGGCGAGCACAATATCAAAACCATGCTGCATCAAGGCGTG210.13520473860417204No Hit
CGTCGAAGTTGGACGATTCGCGACTGGCGTTCTACCGCAACCTCTACCGC210.13520473860417204No Hit
CACGCTATCGGCTCGAAAGTTATTAAACTCAGTAGCCGTCTGGGTGTCCA210.13520473860417204No Hit
GGACTCGCGCTCGATGAAGAAGCGCACGCCCAGGCCACGACCACGGTACT200.12876641771825909No Hit
TGCGGACGCAGGCGCTCGGACGTCACTGCGGCCGGAGCAACGCCCTGGGC200.12876641771825909No Hit
ATACCAAAGATGAGCAATGCAGGTAAAATATTGATATTGCTAGATAGATA200.12876641771825909No Hit
TATCCTGTGAGCGTGACCAATGGCGTGGTAATGATTTAATCTTTGCCAAA200.12876641771825909No Hit
AGACCGATGTGTGCCGTGACATCGGGATGGACCCTGACCGACCTTCGGTG200.12876641771825909No Hit
TATCCATGTAGAACAAGCTATTATCAAGACCTGCGTAGCCGGCACTCATT200.12876641771825909No Hit
AATACGACCTGTACTTGGAATAAACTGCCATAACCCTGCAGCCGATGCGT190.12232809683234613No Hit
ATGCACGGTGGTGCGCAGATCGAAGGTCTGCAACCCCCGGCCAGCGGCAT190.12232809683234613No Hit
GTCCTGCACCGGCTTCGGCCCCGGCGCCCACGACGACGGCGGCCCGCCGC190.12232809683234613No Hit
CTACAACCTTAGCCTATATCAAATTAACACACTAGAAATCGTCCAAGACA190.12232809683234613No Hit
ATCCTGACGGCCGATGAAAGTGACGACCGTCGACCCGTCGGAGACGGATC190.12232809683234613No Hit
AAATCCATCACCGCCGATAGCCAAATCACCATCACAGGCGGACGCAATAT190.12232809683234613No Hit
CTCCTGGGCCCACCTGTGCATCGACCATGCGTCCTGGTATTTCACCCTCC180.11588977594643318No Hit
GTCCATTTTTTACTGAGCAACTTTTATTAACGGTGGAAGCGACTATGAGC180.11588977594643318No Hit
ATCTAGCAATGCCGAGATAAAAATCGCCACACAGGCACGCATATAATGCC180.11588977594643318No Hit
ATTTCGCACCATGTGATGCAAAAAACCATCGGCTTGGATATCCAGTACCA180.11588977594643318No Hit
GTCACAGACAAAGATGGCAATCGGGTGGCTAGACGCTTTGAGCTGTATAT180.11588977594643318No Hit
CTACATCATGAATGGCTCTATTTATGTGGTTAATAGCACTGGTAAACCTG180.11588977594643318No Hit
AAACGTATCAGCGATGAACACCTTTTAACCTATGCCAAAAATAGTCTAGA180.11588977594643318No Hit
AAGTGAGTGGTTAACGCGGTTATTTGCTCTGAGGTAATCGCTGTTTGTGC180.11588977594643318No Hit
GTTTGCCATTAGTGACAAACAGCAAAACGCCCGCTTTCGTGTCTCTGCCT170.10945145506052023No Hit
GTTATAACCACTTTTCAAAGGCTCTTTAGTAAAAATCATCACATGGAAAT170.10945145506052023No Hit
GTCTCTATCGCCGCTAGCCAAGCATGACTGGGCAAAAAATCAAAAACAGG170.10945145506052023No Hit
GGAATCCTTCTCACCTGCCGCCGCAGTGCTCATCTCATTCTGCACGCCGT170.10945145506052023No Hit
GCCTTGCGCGGCATCTTGCGCGCCAGAATTGTCACGCGCCAGCTTGGCGA170.10945145506052023No Hit
TCAGTAGAGATGGGGTTTCACCCATCAGGCTGGTCTCGAACTTTTGACGA170.10945145506052023No Hit
TATCATAGGTCTTTTGTTTTAAATCTTGGGCAATTGGCGCATCGGAACCT170.10945145506052023No Hit
ATCTTGAATTAAATGGGGTGATGTGCCTAGGGTTAGACGATATAAGTCCG170.10945145506052023No Hit
GTTATTGTCAATCCCAGAGCCTACCCCAATGAAATCAGTAGTACCGGGAT170.10945145506052023No Hit
ATAACATTTTACTGTTTTTTATTGAAAAAAGTGACAAGCACTTTATTACC170.10945145506052023No Hit
TCCATGGCATGCGTGCCGTAGCGCATGGAGGGCGACAGGATCGATTCCCC170.10945145506052023No Hit
ACTTTACATCGCTCAAACAAGAAGGTTTGGATGTGATTGCATAACGGGTC170.10945145506052023No Hit
TCCGTACACCCCAGCGCACGACGCATTTGCGTGAATTCGCGCTTGGCCAG170.10945145506052023No Hit
ACCAGGGCCGACGAGCGTTTCGGGAGCGCTTCATTGGCGCGTGATCGGGA170.10945145506052023No Hit
GTACATGTTGAGCTTCGGGACCGCGATGTAGTCCTTGAACCGGCCCGGGA160.10301313417460728No Hit
CTGCCGCTTCTTTGATGGCTTGGAACATCAGATTGCCAGTGATGTCGTTA160.10301313417460728No Hit
CATCTCATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCG160.10301313417460728No Hit
GTGCTCTTCTGCGTCAATCTTCGGCATATTCATTTTCGCTTAATTTTTTA160.10301313417460728No Hit
ACCTAAAATACAAGATAAGCCCCCTAGCTATCGGCCTAAATGATGCTGAC160.10301313417460728No Hit
AACCAGCAGGCACGTGCGTCGTACGACGCTATCCGGGAGCTGCTGATCGA160.10301313417460728No Hit
GTTGTATCTTAACCGCAGGTGCAGGTCAAAGCCCCGCGCGTCAAGCGATG160.10301313417460728No Hit
TGTTGACCTGGTTCGGGAACGCACTCGCTTCCAGGCATAAGCCGCTGCGC160.10301313417460728No Hit
GAGCACCGGTGCGTACTTTTCGATCAGATCCGTTTTCATTGTCAGTCTTC160.10301313417460728No Hit
GTGGTGGAGCGCCGGGGGCTTGCGCCGCACAGTCTGGTCGTCGAAATTAC160.10301313417460728No Hit
GATCAACTCTGACTACAAAGACGTGTTCAGTTTCTCCCTGCTGGTGGTGA160.10301313417460728No Hit
ATCACTGATGATACTGCTACCGGCATCAATGCGGACAATATCACCAACTT160.10301313417460728No Hit
ACGGTGCGCGATGTCGCCACCTTTACCGACCCGCAGCAACCGGCGACGGG160.10301313417460728No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)