FastQCFastQC Report
Tue 7 May 2024
EGAF00003633436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003633436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15532
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGATGCACTGCGCAATATCACCGGCATCTTCACCGACGTGGATGGAA380.24465619366469227No Hit
GGCTTCCGAGCGGCCGATGCGAGCGTTGCTGAAATGCGCGCCGCCGTCGC360.23177955189286636No Hit
TGATAATACCGGTAGAAATGACTATTTATACGGATAGAACATTTAAATTT300.19314962657738863No Hit
AAGCTATCCAGCGTGATGCGCAAGCGCGCGCTTACGCGGCCGCGCCGTAC290.18671130569147568No Hit
TAATAAGTAGGCGTAAATCGTTGGGTGAGTCAGCGTGTTTGATGGCATCG240.1545197012619109No Hit
ATGACAGCTTGCACTTTGGCGATTTGGTTTGAGCTTTATGCCCAATACGA240.1545197012619109No Hit
CCTCCAGGTTGACAATGCCCAGTGGCTCATAGACCGAAGGGCAGGCAAAG230.14808138037599794No Hit
CTCGGCACGCGCGCCGACGTCGGACAGGTCGCCACCGACCCGCGGCGCTG220.141643059490085No Hit
GGTCTGGGTCATAATGGTTAAACGTTATGAAATTAGGCATCAGGTGCGTT220.141643059490085No Hit
GGCGTGGACGTGCGTTTTGCGATTCACCCCGTGGCAGGTCGTATGCCAGG210.13520473860417204No Hit
ATACACGCCTGTTTTGGGATTTTGATAGACATAGCTTTGGCTGTCTTTGA210.13520473860417204No Hit
CGTTGTGCTTGTAGCCGGCGGTCAGCCCCGTCGGGTTGGACGAGGTGCGC210.13520473860417204No Hit
GGGCGTCTCTGGGCTGGAGCGGTGTTGTGGACGTGGGTTCTGGCAAGCCT200.12876641771825909No Hit
ATACCGGCGCTTGCTGGGCGACATCCTGCTGCAATTGCCAGGCGCCGTGC200.12876641771825909No Hit
GAGCGGCGCGCATGGCCCGTTCTTCACCCGCAATATCGTCATCCTGGAGG200.12876641771825909No Hit
GTTTTATATGGGATTAAATACCTTGCCATCGCTGACACAAGGCTTGTTGC200.12876641771825909No Hit
TGACTGGGCGGCCAAGGGCACGGCGCTCCTGGGCGTCAAGGCCGTGATCA200.12876641771825909No Hit
GTGCGGAAAGATCGGCGTTGCCGGCGGCGATCTGGCCGGTGCTCGACGCG190.12232809683234613No Hit
TCCCTACCTCTACGACGGCACACCGGAGTACGAAGCCGACTACCTGGCCA190.12232809683234613No Hit
ATGATAGACAGGGCAGCCTTATGAAACAAATGCTAGAGAACAAACTAGCA190.12232809683234613No Hit
GTCCAGTCATAGCAAAAGCACGGTGCGTAGCGCACAAGAAAATGCAGCCT190.12232809683234613No Hit
GCCCATGACCATCTGACCTAAAATAAACAAAAACACCGTATTGGTAACAC190.12232809683234613No Hit
GGGTAACATGGGTTTTGCCAAAAATTGTGACGACGTCATGCTGCCAAGGT180.11588977594643318No Hit
TCGAAACTGAGATGAGATAAAACATGCCCCAAACTGAAGCCCAACTTGAA180.11588977594643318No Hit
GGGTCGAGTTGTTGGGATGCGGGTTTATCAGCTTTTTGGGTTTTTGGGGT180.11588977594643318No Hit
GTACATATTCAGTCCCATCGACTTTAGTAGTGATACGCACATTTGCGGTA180.11588977594643318No Hit
GCTCATACTTGAGCTAAATATTTTCCCACCCGATTTGCCGATGGGGTTAA180.11588977594643318No Hit
CTCCGAGTTGATCCCGCTGGCACCGGTGGAGCCGCCCCCAACCGAGAAGG180.11588977594643318No Hit
TCTCTATCATGCCATGTGCCACACGAAGCACATATCCATTCTCTTATTCC180.11588977594643318No Hit
GTCCTGTACTGTCACCCCTGTTTTTGAACAATTTGCCACCTCATACGATG180.11588977594643318No Hit
CCCCAGGAGGACCACCAGGTGGTGTGATTCCACTGTCAGTTCGAAGTTGT170.10945145506052023No Hit
GGTTCACCGTGCAGGCGGCAGACCGGACGGCGCGGAGCACGCGGGCAGCA170.10945145506052023No Hit
GTCTACATCCATCGAATAATCTAAGTTAGCAGCCAACCAACGCCCCCAAG170.10945145506052023No Hit
GCGCCATAAGTCTGTCTGACTTAGCTTGATCTTGTGGGTTTGGTTCATCA170.10945145506052023No Hit
AACATACCCTATGAAACCGACCCAAAATCATCAGCCTCAAATATTTGACC170.10945145506052023No Hit
GTTTTCTTCCCATTTAGACCCCTTCACAGCCTGGTCAGCATAATCCTTGA170.10945145506052023No Hit
TTCGGCGGGTGCAATGCGCATGAGGCCGCGGTACTTGGCGACCGTGCGGC170.10945145506052023No Hit
TCGCCGAATCGGGAGCCGATCGCCCCATCGGCGCCCAGCAGGCCTCCGCC170.10945145506052023No Hit
CAATACAGGGGGTGACTTAACCGCATTTAATCCATCCAATATTAAGTTGT170.10945145506052023No Hit
GATACATAATCTTGATGATTTTATCGAAAAATCACAGCAACACTTGTGCT170.10945145506052023No Hit
AAGCGTGGCCCAGCGCAACAACCTGTCGGTGCCGCAGCTCAAGAGCAAGC170.10945145506052023No Hit
TCTTGTTACAAATATAGGGGCCAATGCTCTTTTTGGAGCAATCTCTGGAT170.10945145506052023No Hit
CCCCACCACCGTAAAACGCGGCTGCACGCCAACGAGCAGTTTAATCAATT170.10945145506052023No Hit
GTACGAAGTCGCGCCCGAACTGCGCGCCAGGATGGGGGCACGATGATCGA170.10945145506052023No Hit
GCCCGGACGGGGTCGAACCCGTTGAAGCTGGTCTCGTTCTTCTCTCCGTC170.10945145506052023No Hit
CCGTTTTATTTTCGATAAAGCCATCAAAATATTGCCAAAATTCACGGAAA170.10945145506052023No Hit
CTGTTCATCCTAGACTGGTTGCAAAGCGTCGAGCTGCGCCGCCGTGTGCA160.10301313417460728No Hit
CCAGCGGACGCTGCGCTTCCGAGCCGATGCTGTGCGAGAGCGCCGCCGGC160.10301313417460728No Hit
ATCGAGAATGATCACCTGCGCCGCTATTTAGGCGAACGGTTTTGCCATGT160.10301313417460728No Hit
TTGACAGGCTGGGCAAACTGCTTATTCGCGAGTAGCAAACTACCCCGCTG160.10301313417460728No Hit
ACATGATGGTGGTAATTGATCGGCCGTGACAAACGGGGAGGCATCATGGC160.10301313417460728No Hit
GACTGGCCTAACGCACAGTTGGCGCAGGCCGGCGGATTCGACCACTGCTT160.10301313417460728No Hit
ACATTACAATCGCTTGTCCCACAACAAAAAATCAGTGAAATCGCAGGTCG160.10301313417460728No Hit
ACCCTTGAAAATACTAGGCTTGTGCAATATGCCTTGCAATCTCGTGCTAG160.10301313417460728No Hit
GCGCTGCGCACCGTGATCGAGGAGCACCTGCGCGAGCACCGGATCCGGGC160.10301313417460728No Hit
GCCATGACGATGGCGACCAGCACGGCAACCCGCGCAGGTTCCAGGTTGAA160.10301313417460728No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)