FastQCFastQC Report
Tue 10 May 2022
EGAF00003633443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003633443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29977
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGTGTCACCGAATCTCGTATG5531.8447476398572238No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA4771.5912199352837175No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA3701.2342796143710177No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGTGTCACCGAATCTCGGTTG3511.1708976882276412No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3291.0975080895353104No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGTGTCACCGAATCTCGGATG3011.004103145745071No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2650.8840110751576209No Hit
ATACACATCTCCGAGCCCACGAGACTCGTGTCACCGAATCTCGTATGCCG2630.877339293458318No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGTGTCACCGAATCGCGGTTG2610.8706675117590151No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGTGTCACCGAATCTCGTTTG2580.8606598392100611No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGTGTCACCGAATCGCGTATG2240.7472395503219135No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGTGTCACCGAATCGCGGATG2170.7238883143743536No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA1930.6438269339827201Clontech Universal Primer Mix Long (95% over 21bp)
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA1880.6271474797344632No Hit
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAA1690.5637655535910865No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGTGTCACCGAATCTCGTATT1510.5037195182973613No Hit
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1450.48370417319945297No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA1450.48370417319945297No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA1420.4736965006504987Clontech Universal Primer Mix Long (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1380.4603529372518931No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGTGTCACCGAATCTCGGTTT1300.43366581045468194No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1250.41698635620642494No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGTGTCACCGAATCGCGTTTG1170.39029922940921374No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGTGTCACCGAATCTCGGATT1110.3702838843113053No Hit
ATACACATCTCCGAGCCCACGAGACTCGTGTCACCGAATCGCGTATGCCG1070.3569403209126997No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT1050.35026853921339696Clontech SMART CDS Primer II A (100% over 26bp)
ATACACATCTCCGAGCCCACGAGACTCGTGTCACCGAATCTCGGTTGGCG1050.35026853921339696No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1040.34693264836374554No Hit
ATACACATCTCCGAGCCCACGAGACTCGTGTCACCGAATCTCGGATGCCG1030.34359675751409413No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGTGTCACCGAATCTCGTTTT1010.3369249758147913No Hit
ATACACATCTCCGAGCCCACGAGACTCGTGTCACCGAATCTCGTTTGCCG980.3269173032658371No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG920.30690195816792876Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA880.29355839476932316No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA850.283550722220369No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA850.283550722220369Clontech Universal Primer Mix Long (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA810.2702071588217633No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT790.26353537712246056No Hit
ATACACATCTCCGAGCCCACGAGACTCGTGTCACCGAATCTCGGTTGCCG790.26353537712246056No Hit
ATACACATCTCCGAGCCCACGAGACTCGTGTCACCGAATCGCGGATGCCG790.26353537712246056No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA750.25019181372385496Clontech Universal Primer Mix Long (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTCGTGTCACCGAATCTCGTATGCCGTC730.24352003202455216No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA720.24018414117490078No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGTGTCACCGAATCGCGGGTG690.23017646862594654No Hit
ATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA640.21349701437768956No Hit
ATACACATCTCCGAGCCCACGAGACTCGTGTCACCGAATCTCGTATGGCG620.2068252326783868No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG620.2068252326783868No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG610.20348934182873538No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT600.20015345097908394No Hit
ATACACATCTCCGAGCCCACGAGACTCGTGTCACCGAATCTCGTTTGGCG600.20015345097908394No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT580.19348166927978117No Hit
ATACACATCTCCGAGCCCACGAGACTCGTGTCACCGAATCTCGTATTCCG580.19348166927978117No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA580.19348166927978117No Hit
ATACACATCTCCGAGCCCACGAGACTCGTGTCACCGAATCGCGGTTGGCG560.18680988758047837No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA560.18680988758047837No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGTGTCACCGAATCTCGGGTG560.18680988758047837No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.18347399673082695No Hit
ATACACATCTCCGAGCCCACGAGACTCGTGTCACCGAATCGCGGTTGCCG550.18347399673082695No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA540.18013810588117557No Hit
ATACACATCTCCGAGCCCACGAGACTCGTGTCACCGAATCTCGGATGGCG540.18013810588117557No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT530.17680221503152418Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA510.17013043333222136No Hit
ATACACATCTCCGAGCCCACGAGACTCGTGTCACCGAATCGCGTTTGCCG500.16679454248256997No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT500.16679454248256997No Hit
GGTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAA500.16679454248256997No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA490.16345865163291856No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG480.16012276078326718No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG460.15345097908396438Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG450.15011508823431297No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAG450.15011508823431297No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT440.14677919738466158Clontech SMART CDS Primer II A (100% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACTCGTGTCACCGAATCGCGGTTT410.13677152483570737No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA410.13677152483570737Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACACATCTCCGAGCCCACGAGACTCGTGTCACCGAATCTCGTTTTCCG400.13343563398605596No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA390.13009974313640457Clontech Universal Primer Mix Long (95% over 22bp)
GAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA390.13009974313640457No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA390.13009974313640457No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT380.1267638522867532Clontech SMART CDS Primer II A (100% over 26bp)
ACACATCTCCGAGCCCACGAGACTCGTGTCACCGAATCGCGTATGCCGTC370.12342796143710179No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGTGTCACCGAATCGCGGATT370.12342796143710179No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGTGTCACCGAATCGCGTGTG360.12009207058745039No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA360.12009207058745039No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.12009207058745039Clontech Universal Primer Mix Long (95% over 22bp)
TATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA350.11675617973779898No Hit
ACACATCTCCGAGCCCACGAGACTCGTGTCACCGAATCTCGGATGCCGTC350.11675617973779898No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.11675617973779898Clontech SMART CDS Primer II A (100% over 21bp)
GTATCAACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAA350.11675617973779898No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTT340.11342028888814758No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGT320.10674850718884478No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.10674850718884478No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT310.1034126163391934Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG310.1034126163391934No Hit
GTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAA300.10007672548954197No Hit
ACACATCTCCGAGCCCACGAGACTCGTGTCACCGAATCTCGTTTGCCGTC300.10007672548954197No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAA300.10007672548954197No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA300.10007672548954197No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)