FastQCFastQC Report
Tue 10 May 2022
EGAF00003633501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003633501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31207
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA12674.059986541481078Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG4481.4355753516839171Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG4331.387509212676643Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG2970.9517095523440254Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC2490.7978979075207485Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2420.7754670426506873Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1690.5415451661486205No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1290.4133687954625565Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC1230.39414233985964686No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1210.38773352132534367No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG1120.3588938379209793Clontech Universal Primer Mix Long (96% over 26bp)
GTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCTACATA1080.34607620085237284No Hit
GTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCT1040.33325856378376645No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC1020.32684974524946325No Hit
GTACATGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTCCCA980.31403210818085686No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG940.30121447111225047No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT940.30121447111225047No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT920.2948056525779473No Hit
GTACATGGGCAAGCAGAAGACGGCATACACATCTCCGAGCCCACGAGACC900.2883968340436441No Hit
GTTTTGCTCCTTGGATTCTGAGCCCGGGCATCGCCCCTCGTTTATGAACT890.2851924247764924No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT860.27557919697503763No Hit
GTATATGATGTTGCTCTGCAAGAACCTTTAGGCTAGGCCTTATTAACCTC820.26276155990643124No Hit
GAGTGTCATGTGTCCAAAGTTAAGCTCTGCACCTGTTACACTATAGTCAT820.26276155990643124No Hit
GAAAAACGGTGTGCTTCAAATTGCGCTTGTCGGCTATACAAACGCAGGGA800.25635274137212805No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA800.25635274137212805Clontech Universal Primer Mix Long (96% over 26bp)
AAATGAACCTGCCGGCTGATCGTTTTTAGGATATTGTGAGTAATATAGAT800.25635274137212805No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG740.23712628576921846Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC740.23712628576921846Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT730.23392187650206683Clontech Universal Primer Mix Long (100% over 23bp)
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT720.23071746723491524No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC710.22751305796776364No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.22430864870061204No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC690.22110423943346044No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.21789983016630882No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA670.21469542089915722No Hit
GAAAAGTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCT670.21469542089915722No Hit
CTATGGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTG670.21469542089915722No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG660.21149101163200562Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA660.21149101163200562No Hit
GTATCAACGCAGAGTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTC650.20828660236485402No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA650.20828660236485402Clontech Universal Primer Mix Long (95% over 21bp)
GAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGG640.20508219309770243No Hit
CAGTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACG640.20508219309770243No Hit
CTATAGTCATCGTCCATAACACGATCTAGTGAAACCTTCAAGCATGAATT640.20508219309770243No Hit
GTACATGGGAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCTTGTCGG640.20508219309770243No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG630.20187778383055086Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTAT630.20187778383055086No Hit
GGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGG600.19226455602909603No Hit
ACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGA590.18906014676194444No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC590.18906014676194444Clontech SMART CDS Primer II A (96% over 26bp)
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC590.18906014676194444No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC590.18906014676194444No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT580.18585573749479284No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC570.18265132822764124No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT570.18265132822764124Clontech Universal Primer Mix Long (96% over 26bp)
GCTGTACCCAGGCTCTGCCAGGTGTGGCGCCAGCCGTCGAAGAGGAAGAA560.17944691896048964No Hit
CCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTCC550.17624250969333805No Hit
TTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAGGTGA550.17624250969333805No Hit
GTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAG550.17624250969333805No Hit
GCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC550.17624250969333805No Hit
GTACATGGGTACACATGCAAGTCGAACGGCAGCATAGGAGCTTGCTCCTG540.17303810042618642No Hit
GAACGGAGAAGACCCAGCTCAGCAGGTAGTAGACGGTATCGAGGTCACGG530.16983369115903482No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT530.16983369115903482No Hit
ATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCA530.16983369115903482No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC520.16662928189188322Clontech Universal Primer Mix Long (96% over 26bp)
GCCTGGGGCGTGGCCTTTTCGTCGTCGGCGGCCTTGGCGGCGGCGGAGAG520.16662928189188322No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC500.16022046335758003Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGCA490.15701605409042843No Hit
CCTAAATACACTCGCTGTTGAGATACTAAAATTATCTGTGGATTGCCGGC490.15701605409042843No Hit
GCTTCAAATTGCGCTTGTCGGCTATACAAACGCAGGGAAATCAACATGGT490.15701605409042843No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA490.15701605409042843No Hit
CATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACG480.15381164482327683No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT480.15381164482327683No Hit
GTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA480.15381164482327683No Hit
GTATCAGCCTGTTATCCCCAGAGTACCTTTTATCCGTTGAGCGATGGCCC480.15381164482327683No Hit
GCTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTCGCCAAGTCAGC480.15381164482327683No Hit
CTTATGTATTTGGGGTTTACCGTGGGCTCAATGCCGGCAATCCACAGATA480.15381164482327683No Hit
ACGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGG470.15060723555612524No Hit
CCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCC470.15060723555612524No Hit
AGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACC460.14740282628897364No Hit
GAGTACATGGGCTGCCTTTATCGCTTGATCCATCAGCTTTCGCATGGTGT460.14740282628897364No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA460.14740282628897364No Hit
GTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACC450.14419841702182204No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG440.14099400775467041No Hit
GCCCCGGGTTCGTCCCCTATAAGGGCCTAGTACCTTTCGAGCCCCGCGCG440.14099400775467041No Hit
GTTTTTCTTTCTTCTTTCACGGTATCGGCTTCTATGGCGAATGACAGTGG440.14099400775467041No Hit
GGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAA430.13778959848751882No Hit
AGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTCCCAG430.13778959848751882No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC430.13778959848751882No Hit
GCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCT420.13458518922036722No Hit
TGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTCCCCGGGGTC420.13458518922036722No Hit
GTGGTTCGCATAACTGCCGCTGGGTCCAATATAACACGCAGTCGTCAATC420.13458518922036722No Hit
GTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCC420.13458518922036722No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC420.13458518922036722Clontech Universal Primer Mix Long (96% over 26bp)
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG410.13138077995321562No Hit
GTTCTACAGGGTATATGATGTTGCTCTGCAAGAACCTTTAGGCTAGGCCT410.13138077995321562No Hit
CTATCGGAGTAGCCGCGCTGGTGCCACACGACTACAACCCTCGTAATAGG410.13138077995321562No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA410.13138077995321562Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGGT400.12817637068606402No Hit
CGCTTGGATGCCGCGCCATGTTCATGTCTACCAGCCTGTTATCAGCCTTC400.12817637068606402No Hit
GTCTAAGACACGACACACTGGCTTACGACACGACTAAGTGCTTGCCGACG400.12817637068606402No Hit
ACTGCCAAGCGAGCGTGGTGTGGTGCCCCCGACCTACAACTTGCGCCAAT400.12817637068606402No Hit
TGGTCAGATATTAGACTCCAGGTCGATCATTGGATAACCAACCAGTCTGT400.12817637068606402No Hit
AAACGGAACAGGGCTGACGCCGCTACATATATAGGAAAAGGGAAGGTAGA400.12817637068606402No Hit
GCAGAGTACATGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACA400.12817637068606402No Hit
GTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAGGCTA400.12817637068606402No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG400.12817637068606402No Hit
TACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGGTACCAAGAAAAAA390.12497196141891243No Hit
GATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTT390.12497196141891243No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG390.12497196141891243Clontech SMARTer II A Oligonucleotide (100% over 23bp)
ATCTAACACGAGGATGTTCCTGTTGATACCGTCTGCGATAGGCTAGTTCA390.12497196141891243No Hit
GTGGCAAACAGGTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAA390.12497196141891243No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT390.12497196141891243Clontech SMART CDS Primer II A (100% over 26bp)
GATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGA390.12497196141891243No Hit
TCCTAGTCACCGCGTTTAAAATGAACCTGCCGGCTGATCGTTTTTAGGAT380.12176755215176083No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG380.12176755215176083Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATGCAC380.12176755215176083No Hit
GGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGC380.12176755215176083No Hit
GTTCTATTCCCCAATGACCTTAACTCCGCGATCGACGCTACAAAAAACTC370.11856314288460923No Hit
GTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTGCAGCGTCG370.11856314288460923No Hit
CTGCCATGTTGTGTCCAGTCGCTATCGGAGTAGCCGCGCTGGTGCCACAC370.11856314288460923No Hit
TTTGACACCAGTGCCGCTTTCATGCCGTGGTGTTCGCGCAATAAAGTAAG370.11856314288460923No Hit
GCGTTGAGCGGAATACAGCAATCAATGTCGTCGGAAGATCCTGAATAAAT370.11856314288460923No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAA370.11856314288460923No Hit
GTGAATGGCGACTATGCGCCTAGTCAACCAGATGTGTGCTAGCCCGTCGA370.11856314288460923No Hit
ATCGGTAGCAGCGCCTGTATATCGCGTTGCAGGGCTCTTAAGCTGGATTC360.11535873361745762No Hit
GAGTACATGGGCAAGCAGAAGACGGCATACACATCTCCGAGCCCACGAGA360.11535873361745762No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA360.11535873361745762No Hit
CTTCCTAGTTGGTACAGAACTGAGTGTCATGTGTCCAAAGTTAAGCTCTG360.11535873361745762No Hit
GGCTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATA360.11535873361745762No Hit
CTATAAAAACGGTGCGAGAGTGGCGACAACATACACATCTTGGCAGCTAA350.11215432435030602No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA350.11215432435030602No Hit
GGATGCGACTGCGTATCATATAGGCTGCACACCTTCTGAATGAGTCACAC350.11215432435030602No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG350.11215432435030602No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC350.11215432435030602Clontech Universal Primer Mix Long (96% over 26bp)
TTCCAGAGCTGTACGGTCGCACTGAACTCGGATAGGTCTCAGAAAAACGA350.11215432435030602No Hit
GTATCAACGCAGAGTACATGGGCAAGCAGAAGACGGCATACACATCTCCG350.11215432435030602No Hit
CAATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGTATCGGCTTCTA340.10894991508315441No Hit
GTACATGGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGAC340.10894991508315441No Hit
GAATTGGGGCAAGAGGTCCCGGGGAAACGAAACTGGAAACCGACCGCCGC340.10894991508315441No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA340.10894991508315441Clontech Universal Primer Mix Long (96% over 25bp)
GAAGATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGG340.10894991508315441No Hit
AGTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTATT340.10894991508315441No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT340.10894991508315441Clontech Universal Primer Mix Long (96% over 26bp)
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA340.10894991508315441No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG340.10894991508315441No Hit
ATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGAC330.10574550581600281No Hit
TAACAAGACGGCGCAGGTTGATGCTTCCAATAAACATGATCTTTCTGCTC330.10574550581600281No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA330.10574550581600281No Hit
CTGCTGTGGTGTGTCATACTCGGCTACCTCCTGGTTTGGCGTCAAACAAC330.10574550581600281No Hit
GTCTACGAGAGCGCGGTAGTGGCTTGGGACCAAGCGCCATTTGATGGACC330.10574550581600281No Hit
ATTATGGACAGCTGCAATTTTAACAAACACTGGCTTTTTCCATCTTGTTG330.10574550581600281No Hit
CGCTTGGATCAGTGGGAAGTGCTCACGCGCGGAGCCCACTGGGCGAACAG320.10254109654885121No Hit
CCACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGTG320.10254109654885121No Hit
ATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGATCACG320.10254109654885121No Hit
GAGTACATGGGATGGACAAGACGCACACCACGAATCTCCCGGACGCACTG320.10254109654885121No Hit
GGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAATACTTCAG320.10254109654885121No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGTTC320.10254109654885121No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)