FastQCFastQC Report
Tue 10 May 2022
EGAF00003633502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003633502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31207
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA17105.479539846829237Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12574.027942448809562No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG6682.140545390457269Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG6091.9514852436953247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG3691.1824270195789406Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC2860.9164610504053579Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT2250.7209920851091101Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2010.6440862626974717Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG1480.4742525715384369Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG1440.46143493446983047Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG1410.45182170666837573Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1380.4422084788669209Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1310.4197776139968597No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA1270.40695997692825325Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT1250.40055115839395006Clontech Universal Primer Mix Long (100% over 23bp)
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC1230.39414233985964686No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC1100.3524850193866761Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA980.31403210818085686No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT920.2948056525779473No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT910.2916012433107957No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG880.28198801550934083No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC870.27878360624218923Clontech Universal Primer Mix Long (96% over 26bp)
AAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAG800.25635274137212805No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA780.24994392283782485Clontech Universal Primer Mix Long (95% over 21bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC780.24994392283782485Clontech Universal Primer Mix Long (96% over 25bp)
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG760.24353510430352165No Hit
GTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA760.24353510430352165No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA740.23712628576921846No Hit
CGCTTACACACCGGACGCTTGGATCAGTGGGAAGTGCTCACGCGCGGAGC720.23071746723491524No Hit
GAACTGAGTGTCATGTGTCCAAAGTTAAGCTCTGCACCTGTTACACTATA690.22110423943346044No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG680.21789983016630882No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA670.21469542089915722No Hit
TCATGCGACTGTTCCCCGGGGTCAGGGCCGCTGGTATTTGCTGTAAAGAG660.21149101163200562No Hit
CTTACACACCGGACGCTTGGATCAGTGGGAAGTGCTCACGCGCGGAGCCC660.21149101163200562No Hit
GTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGCCTT650.20828660236485402No Hit
CGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAA640.20508219309770243No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC630.20187778383055086Clontech SMART CDS Primer II A (96% over 26bp)
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA630.20187778383055086No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG620.19867337456339926Clontech Universal Primer Mix Long (96% over 26bp)
GCCTATGGCTGAGTATGTAACGCTATGGCGTGCTAGTCGTCTCATATACG620.19867337456339926No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC590.18906014676194444No Hit
GGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATCTTTT580.18585573749479284No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA580.18585573749479284Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GACTAAGAGCATGTGGTGGATGCCTTGGCGATGATAGGCGACGAAAGACG570.18265132822764124No Hit
CGTTATAACGGCCACTCAGTGGTTCGTCACGCGCAGCCCCGGGTTCGTCC560.17944691896048964No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.17944691896048964No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC530.16983369115903482No Hit
TATCAACGCAGAGTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTCT520.16662928189188322No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC510.16342487262473163Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATCGGCGCGCTGGTGCTGCATTCGGGGCTTCTGTTCGCGAGCGGGG500.16022046335758003No Hit
GTTCTGCATCGCCCACTTCCAAATACTTCTGGCCATGGTCGTCATCGTTG500.16022046335758003No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT480.15381164482327683No Hit
TAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAA480.15381164482327683No Hit
CTTATGTATTTGGGGTTTACCGTGGGCTCAATGCCGGCAATCCACAGATA480.15381164482327683No Hit
AGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCC480.15381164482327683No Hit
GTATAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG470.15060723555612524Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.15060723555612524No Hit
CCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCC470.15060723555612524No Hit
CTATAAGGGCCTAGTACCTTTCGAGCCCCGCGCGTACTAGGCAGATAAGA470.15060723555612524No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG470.15060723555612524No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.15060723555612524No Hit
ATACTGAGCGATGCAGCGTCATTACGCGAGTGCATGTGCCATAATCCGAG460.14740282628897364No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC460.14740282628897364Clontech Universal Primer Mix Long (96% over 25bp)
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG460.14740282628897364No Hit
ATTATGGAGTGAGGCTGTACCACGATCCGGCAGGTACGCTGCAACAGACC450.14419841702182204No Hit
CCCCGGGACCTCTTGCCCCAATTCCTCCGCCTTGCCGGGAAAGGTTGATC450.14419841702182204No Hit
CATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCC450.14419841702182204No Hit
GTGCGATACGGTATGCGGCGGGAGGGCTCGTTCATCCTTCGATCGTGCCA450.14419841702182204No Hit
ATATCATATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCT440.14099400775467041No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC440.14099400775467041No Hit
GCTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATAA440.14099400775467041No Hit
CGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGG440.14099400775467041No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG440.14099400775467041No Hit
TAAAAACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGGTGTGACG440.14099400775467041No Hit
ATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGAC430.13778959848751882No Hit
CTCTCACAGCCAATAACTTCAACAACTTCCCTATCTTTAATCCTCTCACT430.13778959848751882No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT420.13458518922036722Clontech Universal Primer Mix Long (96% over 25bp)
TTTATGAACTAGCCTATCGCAGACGGTATCAACAGGAACATCCTCGTGTT420.13458518922036722No Hit
TAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTGCAG420.13458518922036722No Hit
TAAGCGCGGTGGCCTCCCCTATCCCCTACGAGACTACCCGGATCGATGAC410.13138077995321562No Hit
GTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATCTTTTCCCTAT410.13138077995321562No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT410.13138077995321562Clontech Universal Primer Mix Long (96% over 26bp)
CTCGTGGAAGAGCTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTC410.13138077995321562No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.13138077995321562No Hit
TTTCTGCTCCGCTGAGAAGTACACCTTTTTGCTAGCATCTCGCACGGCAA400.12817637068606402No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG400.12817637068606402Clontech Universal Primer Mix Long (96% over 26bp)
ACATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTA400.12817637068606402No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC400.12817637068606402No Hit
GGTTATCAGACATGCCGCCCGCATTGGCAATCTTCCACGCCAGGTACAGC390.12497196141891243No Hit
GAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGG390.12497196141891243No Hit
GTACAGAACTGAGTGTCATGTGTCCAAAGTTAAGCTCTGCACCTGTTACA390.12497196141891243No Hit
GAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCA390.12497196141891243No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT390.12497196141891243No Hit
GTGCCGACACGAAGCAGCTCAATATCTAACACGAGGATGTTCCTGTTGAT390.12497196141891243No Hit
TTTCTGGAAACTGCCTCGTCATGCGACTGTTCCCCGGGGTCAGGGCCGCT380.12176755215176083No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA380.12176755215176083No Hit
GTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCT370.11856314288460923No Hit
GCACCTGTTACACTATAGTCATCGTCCATAACACGATCTAGTGAAACCTT370.11856314288460923No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC370.11856314288460923No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA370.11856314288460923No Hit
GAAGCACACCGTTTTTCTTTCTTCTTTCACGGTATCGGCTTCTATGGCGA370.11856314288460923No Hit
GATTTAATTCTGCATTTAATTGATTCTTCAAATGAGGATTATGCGGGACA370.11856314288460923No Hit
ATACATAGGTGTATGGAGCGAACCAGGGGAACTGAAACATCTAAGTACCC370.11856314288460923No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAA370.11856314288460923No Hit
GACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACACAAAAACGGA360.11535873361745762No Hit
ATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTCTTTTA360.11535873361745762No Hit
CAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGCA360.11535873361745762No Hit
CTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTGATCCCTTCCG360.11535873361745762No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT360.11535873361745762Clontech SMART CDS Primer II A (100% over 26bp)
TTTTAAGGGTAAAACGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAG360.11535873361745762No Hit
CCTAAAGTATGCTGCAAACATATGCAGATCCCCTGAGACAGAGTCGCTAT350.11215432435030602No Hit
TACTAGGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGA350.11215432435030602No Hit
ATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGATCACG350.11215432435030602No Hit
CCTTTAGGCTAGGCCTTATTAACCTCTCTCTCCTACATTTGCTATGCGTC350.11215432435030602No Hit
GTGTATAATGTCTCGGACCCGTCGCAACGGTTGTTAATATGACAGGCCGC350.11215432435030602No Hit
TCGTTGTTCGTCGACTTCTAGTGTGGAGACGAATTGCCAGAATTATTAAC340.10894991508315441No Hit
TCTTTAATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCA340.10894991508315441No Hit
GCTGCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCC340.10894991508315441No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT340.10894991508315441No Hit
CTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTC340.10894991508315441No Hit
AAGTTGTAGGTCGGGGGCACCACACCACGCTCGCTTGGCAGTAACGCGAG340.10894991508315441No Hit
GGACCGATGACAGTTCACAGAGCATCCTTATACAGTACGCCGCGACCGTA340.10894991508315441No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT340.10894991508315441No Hit
GTACATGGTAGTTCAGCCAATAGATGCCTAGTACGCTGACGGCATTCAGA340.10894991508315441No Hit
CGTATAGGGACGCCCCCTTCTCGCGTTACTGCCAAGCGAGCGTGGTGTGG330.10574550581600281No Hit
GTACATGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATGCA330.10574550581600281No Hit
GTATCATCGCCAGCATCTCCTGTGAGGATGTTGGCGTTTGCATCACCGGT330.10574550581600281No Hit
GTACACCTGCTCGCTGATCTAAGTCTTAACTCTGTTATGTAGTGAATGGG330.10574550581600281No Hit
CTCTTGAAGCGAGAGAACCAAGGTGCTGGCTATTGTCCGCCCCGTCTACG330.10574550581600281No Hit
AATAATGATTGCCCATATGTCTTATGGAGCTTTGTCTTTAAACGCTCACC330.10574550581600281No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC330.10574550581600281No Hit
TCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATTT330.10574550581600281No Hit
CCTGTTTGCCACGCTGGACCCGATGACCAAAAAAATGGTCCTGCCAAGCG330.10574550581600281No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG330.10574550581600281Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGTGAATGGCGACTATGCGCCTAGTCAACCAGATGTGTGCTAGCCCGTC320.10254109654885121No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA320.10254109654885121Clontech Universal Primer Mix Long (95% over 22bp)
AATCAACGCCGCAACTTTACGTCGGATTAGCTCTTCTTTGATTAGCATGA320.10254109654885121No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)