FastQCFastQC Report
Tue 10 May 2022
EGAF00003633527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003633527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15562
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGCAAGCAGAAGACGGCATACACATCTCCGAGCCCACGAGACT1170.7518313841408559No Hit
GTACATGGGCAAGCAGAAGACGGCATACGAGATCTTGCTTCGTGACTGGA840.5397763783575377Illumina PCR Primer Index 1 (96% over 28bp)
GTATCAACGCAGAGTACATGGGCAAGCAGAAGACGGCATACACATCTCCG480.30844364477573577No Hit
GTATCAACGCAGAGTACATGGGCAAGCAGAAGACGGCATACGAGATCTTG350.22490682431564069Illumina PCR Primer Index 1 (96% over 28bp)
TATCAACGCAGAGTACATGGGCAAGCAGAAGACGGCATACACATCTCCGA350.22490682431564069No Hit
GGAATGAAATGTACTGCAAAGGACTCGAATTTCATGAAACGGAATGGAAT320.20562909651715716No Hit
ATCTAGAGACATTTGCGGTTGTCAGGAATCAAGTAAGGATGCTACTGGTA300.19277727798483485No Hit
GTACATGGGCAAGCAGAAGACGGTATACACATCTCCGAGCCCACGAGACT280.1799254594525125No Hit
ACATTAGACAGATCAACGAGACAGAAAGTTAACAAGGGTATCCAGGAATT270.17349955018635135No Hit
AGTAGAAACAATGTAAATGTCCACCACCTGATGAATGTCTAAACAATGAA270.17349955018635135No Hit
GAGCTTCACCTTGTTGCCCAGGCTGGTCTTGAACTTCTGACCTCAGGTGA270.17349955018635135No Hit
GGAGTATGTGTTTTACTCCATAAGCCACATCAAACTGTTTTTCGAAGCAG260.1670736409201902No Hit
ATCCCATATTAGTCCTTGTAACACAGATTTGGTTCTGTACACAGCCATTT260.1670736409201902No Hit
GCAGAAGACGGCATACGAGATCTTGCTTCGTGACTGGAGTTCAGACGTGT260.1670736409201902Illumina PCR Primer Index 1 (96% over 25bp)
ACTCAGGACATGTGGACAGAATTTGACAGGAGAGTGGGATGAAAAAAACA250.16064773165402904No Hit
GTCAGGTACTGTTCTTATTACTATTCATGTACTGGTTATTTTAATCCCAC250.16064773165402904No Hit
CCAATAACCTTCTCTAACAGACCAATCAAAATGTCCTTTTCCATAATATG250.16064773165402904No Hit
CGATTGGAATGAAATCGCATAGAATGGAGTGGAATCAAATGGAATTGAAT240.15422182238786789No Hit
CCAGAGAAACTCTGAGATAATCAACAGCCACTAAACGTATGGTAAGTTGT240.15422182238786789No Hit
GATGTCAGCTGTCCTAATTTTTCACCTTCCAGAGCATTATGAGTTCACTG240.15422182238786789No Hit
AACCCTGTCTCTACTAAAAATACAAACAGTTAGCTGGGCATGGTGGCAGG240.15422182238786789No Hit
AATCCACACATTGCAAAGCATTTTTAGAGATTGCTTTTTCATTGTTTTCA240.15422182238786789No Hit
GTATATACATAAATTTAGTTGGTAAAACAGTCTTGGGGTCTGAGAGAATT240.15422182238786789No Hit
ATAGAAAGCCATCCTGCAATCTGCGCCCCTGCTGGGGCAAGTCTAGGAAG230.14779591312170673No Hit
GTCCTAACCTGGGGTAGGAGATGTCCAGGACAAGTAAAGTGGATGTTGGC230.14779591312170673No Hit
CTCTGACACTAAATCTCAGATGTTTTGAATAAACTGGGAAGTTTATCTCA230.14779591312170673No Hit
GAGAAATTCTAATTCCAATTAAGGAGTTGATTGTACCGGTGTAAAGAGGG230.14779591312170673No Hit
GGAGGAGCATGGTACAAGAGGATATCAGAAACATAGCCAGGAAAGAGAGC230.14779591312170673No Hit
GATTCTGGAAGACAACCTAGGCAATACCATTCAGGACATAGGCATGACAA220.14137000385554557No Hit
TGTATTAAGGTACTCCCTTCCAGAGTATTAAAGAACAGAGCTCGCACAAT220.14137000385554557No Hit
CCTATACTTGCTGTACTGGATGTCATCTCCATACTGAAACAGACCCATCC220.14137000385554557No Hit
ATATATTACAAGGCAATAGTAACCAAAATAGTATGGCATTGCTATAAAAA220.14137000385554557No Hit
GCATTGAATCAGTAGATTGCTCTGCATGGTATGGTTATTTTAACAATATT220.14137000385554557No Hit
GGGATTAACTCATTAAGAATAAAGGAAACATCTGTGTTGCTTAAATGACA220.14137000385554557No Hit
GATCCTGCACTGCTATGGTCACTGATGATTATAATTTGAAAAATTATCTT220.14137000385554557No Hit
GTCTAGAACCCAGCCCCCTTTACTGCTGTCTTCCTAGTTTATAGCTGGGG220.14137000385554557No Hit
GACACTTTTGCAGTTGAAGGAGTCGGGATGGGACATAGGTCTTCTGGCCA220.14137000385554557No Hit
GTATAAGATAGTGTAAAGTTGTACTGGGTAGCAGAGAGCAGCTTAGGATA220.14137000385554557No Hit
GGGTGACAGAGCGAGACTCCATCTCAAAAAAAATAAAATGAAATGAAAAA220.14137000385554557No Hit
GACCTGTAATTTTCACTGGTTTCTAACATTTCTTTTCTGAGATAATACTT220.14137000385554557No Hit
CATACAAGTTATTTTACCTGTGTGCCTGAGTTTCCTCACTTGTGGAATGG220.14137000385554557No Hit
ATATTACTCACTTTGAGAACAAATCCATTAGGATTTCTCTCTCCTTCAAT210.13494409458938442No Hit
ATATTATCACATTAGTAAGCTATTTCTCCCACTTAGAAACCCTATCCTGT210.13494409458938442No Hit
GAACAATACTGTGCTGAGAAGTATACCCTCAGCCCATTATTTTTCCAGTG210.13494409458938442No Hit
ATCAAATGGCATGGAATAAAATAGAATGAAAGAGAATCAAATGGAATTGA210.13494409458938442No Hit
CTCCTGACCTCAGGTGATCCACCTGCCTCAGCCTCCTAAAGTGCTGGGAT210.13494409458938442No Hit
GTACTGGGATTACAGGCGTGAGCCACCACGCCCAGCCATGACAGCAAACT210.13494409458938442No Hit
CTGAATACTTGGCAGCTAGGTGGGTGACAACAGAGGATTGAGGATTTTTA210.13494409458938442No Hit
CACCATACCCTTATTTTTGGAATAGTTTCCACCTGTGGTTTGTTGAATTT210.13494409458938442No Hit
ATGTTTCACTTTAGTTCTCTTTTTCAGCCTCCTTTCTGAGCCCTTAGCAA200.12851818532322323No Hit
CTGTGAAGTGTATGCAGCCATCACAAATAAGTGCCTCAGACAGTTTCTTT200.12851818532322323No Hit
TATCAACGCAGAGTACATGGGCAAGCAGAAGACGGCATACGAGATCTTGC200.12851818532322323Illumina PCR Primer Index 1 (96% over 28bp)
CTCCTAAAGTGCTGGGATTATAGGCGGGAGCCACTGTGCCTGACCTTGTC200.12851818532322323No Hit
GTCTTACTCTTCACCCATGCTGGAGTGACATGGTGCAATCTGGGCTCACT200.12851818532322323No Hit
AGCTGGGACTACAGGCGCCTGCCACCGCGCCCAGCTAATTTTTTTGTATT200.12851818532322323No Hit
AATATCACTGATGAATATTGATGCAAAAATTTCCAAAAAAATACTAGCAA200.12851818532322323No Hit
AGCTAAAACAATTAAAAGTCTTAGAATAAAACATAAGAGGTAAATCTTCA200.12851818532322323No Hit
ACTCAGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCAGAGGTT200.12851818532322323No Hit
GAAAATGAGGGACTCCTTTCTAACTCATTTTATGAAACCATCATCATCCT200.12851818532322323No Hit
ATTTAATATTATCCGAGACATTAAGTAATCTGAGAACAAAAACGTTGATT200.12851818532322323No Hit
GATCCCCGACTGCTCGGGGAGACTGATTTGAGTAATAATAAAACTCCCAT200.12851818532322323No Hit
CTTTAAAAATAAAAACCACCAGAAAAATAGAATTCAATTACAGGAGTCAG190.12209227605706208No Hit
GTCAAGAGATCGAGAACATTTTGGCCAACATGGTGAAACCCTGTCTCTAC190.12209227605706208No Hit
CTATCTTGCTTGCCCAGAGGTCCCATTCATGGCCCTTCCAGAGTTTGTGG190.12209227605706208No Hit
ACTTTGTGGTTTACATTTAGGTCTGTGATCCATTTTGAGTAAATTTTTGT190.12209227605706208No Hit
AGGCTGGAGTGCAATGGCGCGATCTCGGCTCGCTGCAACCTCCGCCTCCT190.12209227605706208No Hit
GAGTGTCAAGGACATGGTAGGTCCCAGTTGTCTCCTAGGAGTGTAGACAT190.12209227605706208No Hit
ATTCTAAATTCTAGGCTTAGAATTGTAAACTTAGAACTCTAAACTTAGAA190.12209227605706208No Hit
GAGTCACCTACTCTATATTCTAAGACACACCAGGAGGTAAGGGTACAGAG190.12209227605706208No Hit
GTATCCGGCGGCTCCGCGGATCCGCTGGTCGCCGCCTGAGCAGAAGGCGT190.12209227605706208No Hit
GAGTTGAACATTCCCTTTGACAGAGCAGTTTGGAAACTCTCTTTGTGTAG190.12209227605706208No Hit
GGTCTTGGAACTCCTGGGCTCAAGAGGTCTTCCTGCCTCAGCCTCCTAAA190.12209227605706208No Hit
CAACCTATTCTCTGTGCCAAAGGTTGATGCTGTTGTTTGTCCAAATGTGT190.12209227605706208No Hit
TATATATACACATACCACATTTTCTTTATCCACTTGCTGATTGATGGGCA190.12209227605706208No Hit
CTCTTGGACACCCCCAGAACACTCTATTCTGATGCTTTGTTTGCTAGCCT180.11566636679090092No Hit
CTTATATTGTCTTTCCCCAGGATATGAAAAATTATTTTTGAGCATCATGC180.11566636679090092No Hit
CTTCAAATAAAAACTAGACAGAAGCATTCTCATAAACTTGTTTGTGATGT180.11566636679090092No Hit
CCACTGCATTTCCACTCACATTGAGAAATACATATTCTTAGGTTTCTTCA180.11566636679090092No Hit
GAATGAAAGCTTCAAACAATAACTGAGCAGCGTGAGAGACCTCTGAGGGT180.11566636679090092No Hit
GGTGTGGAAGGTATAGCTGAAATCCCATACTCAAGTCTCTCTGAGCCTGA180.11566636679090092No Hit
CTGCCATGCTGGCCAGGCTCACAGTCACAGCTATTCTGTGTTCATGAGAC180.11566636679090092No Hit
CTGTGAGATGAAGGCACACATCACCAAGAAGTTTCTCAGAAACCTTCTGT180.11566636679090092No Hit
ACATTATATACTGTACATAAAATATGAAATTACATCAAATATATTTTATA180.11566636679090092No Hit
GGAGTTTGAGACCAGCCTGGCCAATATGGTGAAACCTCGGCTCTACTAAA180.11566636679090092No Hit
GGCATAAGAAATTATAAGAGTATTGATTGGGGAAGTGATAAATGTCCATG180.11566636679090092No Hit
GTGCAATACAACAGACTAGAATGTCCACTGCAAACATACACCATGCTGTG180.11566636679090092No Hit
ATTCTTTATAAATTACCCAGTCTCAGGTAGTTTGTTATAGCAATGTGAGA180.11566636679090092No Hit
GAGCAGACTTGAAACACACTTTTTGTGGAATTTGCAAGTGGAGATTTCAG180.11566636679090092No Hit
GTACATGGGCAAGCAGAAGACGGCACATCTCCGAGCCCACGAGACTCTGT180.11566636679090092No Hit
GCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATC180.11566636679090092No Hit
GTATAGGATTAAAGCCTTGAATTACATACTTCTGAATATGGGTAAATTAT180.11566636679090092No Hit
ATAACTCTGTGAGATACATAAACAAATACCTAACATTATATAATTTATTA170.10924045752473974No Hit
CTCCAGCACCTACTGCGTGCGGGCACTGGAGAAATCGGTATGAACAAGAC170.10924045752473974No Hit
CATCTGGCATGGCAAGGTCGACCCGATGCATCCTCTGGCGAAGGTCTTCA170.10924045752473974No Hit
AGGATAGACGGATTTCTCAAAGTCAAGTCAACTAATTCACCCTCACATGT170.10924045752473974No Hit
AGCTACTATTAAAAAATCAAAAAACAACAGATCTTGGGAGGCTGTGGAGA170.10924045752473974No Hit
TACCAATTGTAAATGAGATTATTTTTTCAGACTGTTCATTGTTGGCATAC170.10924045752473974No Hit
GTGTCTAAGTGTATTGATGTTTATGAAGCCATGTAAGATGTGGTTCCTGC170.10924045752473974No Hit
AGCCATATGGAACTGTAAGTCCAATGAAACCTCTTTTTGTTCCCAGTTTT170.10924045752473974No Hit
ATATTACCCAAGTGGTTTGGAAATGTTCATATAGCTTCCAAAAGTGCTGG170.10924045752473974No Hit
GAGCCAGACTGACCTAGATTCAAAACTCAGCTCTTCTGCTTACTAACTCC170.10924045752473974No Hit
GAATGGAATGGAATGCAATGGAATCTAATGAAACGGAAAGGAAAGGAATG170.10924045752473974No Hit
CCTATGTCCTGAATGGTATTGCCCAGGTTTTCTTCTAGGGTTTTTATGGT170.10924045752473974No Hit
CTTTTCCTTTCACTTCAACACTTAGAGGCCATTGTGGGGTTATTAACTGA170.10924045752473974No Hit
GGTAAGTGCAAAGTGAAAGCTGTTAGAGCTGGAATATAGGGTCTGAGACC170.10924045752473974No Hit
CAAAGAACACAGGTCAGCCAGGCCTGGGAGTGAGTTACCCATAAAGCCTC170.10924045752473974No Hit
TACCTATACTTTGTGAAGTGTGTTTAATCTCTGAGTTATGTACATCAAAA170.10924045752473974No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.10924045752473974No Hit
TATCATGCCAGTGCACTCCAGCTTGGGTGACAGAGTGAGATCCTGTTCCA160.10281454825857858No Hit
GTATTGTCTCCATTGTGCCACCTGCAGTGAGCTTAACATTTGGATATAGG160.10281454825857858No Hit
CCCCCATGGCTGCTGGGCATGAATCCATTTCCCACTCCGTGCTGTTGCTT160.10281454825857858No Hit
CTATAAATCATTATGACTTCTTTTTATCATAGACAAGCGTCAAGAGCAAT160.10281454825857858No Hit
ATTAAAAGGGAGTTAAAACTGTAATCCTTAAATATGCGAAAGTTACATTG160.10281454825857858No Hit
ATCTGGGCTCACTGCAACCTCCACCTCCCAGATTCAAGCAATTCTGTCTC160.10281454825857858No Hit
ATCTAGTGTCCAAGACTGGAGCTCAGGAGTATGCCTTTTGTTTCTAAAAA160.10281454825857858No Hit
ACAGTGATGACATCATGCTAATTGATAGGACATTGTGATAGAGAACTAGC160.10281454825857858No Hit
GAATTGAAAGAGGAGCATGATAGAAAGCTGGAACCGGAATTTAAGAAATG160.10281454825857858No Hit
AGCAGAAACAGTTGGCACTCAGTGAGTAGGCTCCAAAGCTCTGGCTCAAG160.10281454825857858No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)