FastQCFastQC Report
Tue 10 May 2022
EGAF00003633543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003633543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27002
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA17756.573587141693207Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG6542.422042811643582Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG6192.2924227834975186Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC3621.3406414339678543Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG3461.2813865639582251Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3301.2221316939485964Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT2160.7999407451299904Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT1570.5814384119694838Clontech Universal Primer Mix Long (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG1430.5295904007110585Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1330.4925561069550404Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG1220.45181838382342043No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG1040.38515665506258795Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG1020.37774979631138433Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC1010.3740463669357825Clontech Universal Primer Mix Long (96% over 25bp)
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG960.3555292200577735No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT940.34812236130656987No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG940.34812236130656987Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA910.33701207317976445Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.3184949263017554No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA800.29627435004814456Clontech Universal Primer Mix Long (95% over 21bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC790.2925709206725428Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA790.2925709206725428No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT760.2814606325457374Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC710.26294348566772835Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACACAGCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGA680.2518331975409229No Hit
TCTTTAATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCA650.24072290941411748No Hit
CACTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCCAGCGTG650.24072290941411748No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG630.2333160506629139Clontech Universal Primer Mix Long (96% over 26bp)
ATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCA590.21850233316050663No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC580.2147989037849048Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACC560.2073920450337012No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG550.20368861565809943No Hit
TACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGGTACCAAGAAAAAA550.20368861565809943No Hit
TATCAACGCAGAGTACATGGGATCGGAGCAGGGTGAGTCGGCCCCTAAGG550.20368861565809943No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC530.19628175690689578No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA520.19257832753129397No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC510.18887489815569217No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC500.18517146878009036Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA490.18146803940448855No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA490.18146803940448855Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGAT460.17035775127768313No Hit
GTACATGGGAGGTCCCGCCGTACGCTGAGGACCACCTGAAACGGGCATCG460.17035775127768313No Hit
GTACAGGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA460.17035775127768313Clontech Universal Primer Mix Long (96% over 28bp)
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG440.1629508925264795Clontech Universal Primer Mix Long (96% over 26bp)
GTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCGTTACT440.1629508925264795No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACA440.1629508925264795No Hit
GTACATGGGCAAGCAGAAGACGGCATACACATCTCCGAGCCCACGAGACA440.1629508925264795No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.1629508925264795No Hit
GGATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG420.1555440337752759Clontech Universal Primer Mix Long (96% over 26bp)
TTCCAGAGCTGTACGGTCGCACTGAACTCGGATAGGTCTCAGAAAAACGA420.1555440337752759No Hit
CTCCTCATCTTTAATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGC420.1555440337752759No Hit
ACACTATAGTCATCGTCCATAACACGATCTAGTGAAACCTTCAAGCATGA410.15184060439967412No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT410.15184060439967412Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTATAGTTACGG400.14813717502407228No Hit
TGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAAC390.14443374564847047No Hit
AATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGCAA380.1407303162728687No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT380.1407303162728687Clontech Universal Primer Mix Long (96% over 25bp)
CAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTA380.1407303162728687No Hit
CTTGGTTATTCTCGCTAGACACGGTTCGGGAAGTGGAGCATCGTTAGCTG380.1407303162728687No Hit
GTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAG370.1370268868972669No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA370.1370268868972669Clontech Universal Primer Mix Long (96% over 26bp)
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA370.1370268868972669No Hit
GAGTACATGGGGGGAATTCGAGCTCTTTCGACGTTTTGAAGGAGGGTTTT370.1370268868972669No Hit
TAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGA350.12962002814606324No Hit
CCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTC350.12962002814606324No Hit
GAATGCAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTAT350.12962002814606324No Hit
CACTAACAATGTAGAAACAATGTAGAAACAATCGAGCTTCCCCCACGATG350.12962002814606324No Hit
CCTCACGGTACTGGTTCACTATCGGTCGATTACGAGTATTTAGCCTTGGA350.12962002814606324No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT350.12962002814606324No Hit
GCGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC340.12591659877046146Clontech Universal Primer Mix Long (96% over 26bp)
GAAATCAACATGGTTCAACCGCCTGACGAGTGCTGACAGCTATGAAGAAG340.12591659877046146No Hit
CTACTGGGGCGAGTATTCCCAATGTATGGTCAGAATATCCCACAGGGATA340.12591659877046146No Hit
GATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACG340.12591659877046146No Hit
CTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTC340.12591659877046146No Hit
GATCCCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAG340.12591659877046146No Hit
GAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCC330.12221316939485966No Hit
GTCCGTCCGACTTCACTGCCCCCTTTCAGCCTTTTGGGTCCTGTATCCCA330.12221316939485966No Hit
GATGGACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGATAATTGCGCG330.12221316939485966No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA330.12221316939485966No Hit
CTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGAA330.12221316939485966No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA320.11850974001925782No Hit
TCAACACACAGCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAA320.11850974001925782No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG320.11850974001925782Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACACAAAA320.11850974001925782No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG320.11850974001925782No Hit
ACATGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCACA310.11480631064365603No Hit
ATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTCTTTTA310.11480631064365603No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC310.11480631064365603No Hit
GGTTAATATTCCTGCACCGTCGTTAGATGCGATGGGGGGACGGATCGCGG310.11480631064365603No Hit
CGATAGTGATGTAGAGATCTTAAATCCTGATCTTCATATCGCGACTCTTG310.11480631064365603No Hit
GAAAAAAACGAACCTTTGGGTTCCAGAGCTGTACGGTCGCACTGAACTCG310.11480631064365603No Hit
AGATATGGTGGAGCTGATTGAGGTGATGCGCGCCGATTTCGAAGAACATA310.11480631064365603No Hit
GAAGACACTTCGTGCCGACACGAAGCAGCCTCAATATCTAACACGAGGAT300.11110288126805422No Hit
GGTCGGGGGCACCACACCACGCTCGCTTGGCAGTAACGCGAGCAGGGGGC300.11110288126805422No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC300.11110288126805422Clontech Universal Primer Mix Long (96% over 25bp)
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT300.11110288126805422No Hit
ATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGATCACG300.11110288126805422No Hit
GAGATGCGTAGCTGATGGGAAGCAGGTTAATATTCCTGCACCGTCGTTAG300.11110288126805422No Hit
CACCGGCGCACGCCACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCC300.11110288126805422No Hit
AGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGCTGATCTCCTC300.11110288126805422No Hit
ATTCTCCTGCCTCAGCCTCCAGAGTAGCTGGGACTACAGGCGCCCGCCAC300.11110288126805422No Hit
CCCTTGTCCCGTCAGACGCGGCAGTGCATATTGCAGCTGAGCCAGCTCAA300.11110288126805422No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG300.11110288126805422Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCAGCCACGAGTCACGGGGTCTAACGCCTACCAGTACTGATTGCCGAG290.1073994518924524No Hit
GAGGAATAGGTACCAAGAAAAAAACGAACCTTTGGGTTCCAGAGCTGTAC290.1073994518924524No Hit
ACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGC290.1073994518924524No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG290.1073994518924524No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC290.1073994518924524No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC290.1073994518924524Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGGGATAACTAGTTAAGACTAGCTAATACCG280.1036960225168506No Hit
CCTTAGGACCGTTATAGTTACGGCCGCCGTTTACTGGGACTTCAATCAAG280.1036960225168506No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCTCTTGCTTCAACAATAACGT280.1036960225168506No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA280.1036960225168506No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT280.1036960225168506No Hit
GCCTAGACAAGAGTTTCATCACTTGGTTGATCAGTTCAGAGATGTGTCAC280.1036960225168506No Hit
CTCTTGAACCGTGTTAATACCCGCACGCTTTAAGCAGTTGTAAGAACGAA280.1036960225168506No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA280.1036960225168506No Hit
GTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACCAGTAC280.1036960225168506No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)