FastQCFastQC Report
Tue 10 May 2022
EGAF00003633544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003633544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27002
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA13224.895933634545589Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12354.573735278868233No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG5762.133175320346641Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG5341.977631286571365Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4771.7665358121620622Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG4141.5332197614991483Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC2700.9999259314124879Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2030.7517961632471669No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1880.6962447226131397No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1770.6555069994815199Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1580.5851418413450856Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT1470.5444041182134657Clontech Universal Primer Mix Long (100% over 23bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG1320.4888526775794386Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG1320.4888526775794386Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1260.4666321013258278Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA1230.4555218131990223Clontech Universal Primer Mix Long (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1140.422190948818606No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC1060.39256351381379156Clontech SMART CDS Primer II A (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA1060.39256351381379156Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1000.3703429375601807Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG920.34071550255536626Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT910.33701207317976445No Hit
ATATTAAGGTGTTCAGTTAAAATCTTTGAACCAAGCGCAATTGCTTCTTT910.33701207317976445No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC890.32960521442856083No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG830.30738463817495Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.30368120879934823No Hit
GGCTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATA820.30368120879934823No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC800.29627435004814456Clontech Universal Primer Mix Long (96% over 26bp)
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC790.2925709206725428No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC780.28886749129694095Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA740.2740537737945338No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC680.2518331975409229Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA660.2444263387897193Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG630.2333160506629139Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC630.2333160506629139Clontech Universal Primer Mix Long (96% over 25bp)
GGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGC620.22961262128731205No Hit
GAGCTGTACGGTCGCACTGAACTCGGATAGGTCTCAGAAAAACGAAATAT610.22590919191171022No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA600.22220576253610844Clontech Universal Primer Mix Long (95% over 22bp)
GGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.22220576253610844No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT590.21850233316050663No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT570.211095474409303No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAA570.211095474409303No Hit
TCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACACAAAA570.211095474409303No Hit
GATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAG560.2073920450337012No Hit
GAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTATAGTTACGG550.20368861565809943No Hit
GCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTG540.1999851862824976No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT530.19628175690689578No Hit
GCTATAACCCGCAGACCCGAGCGAAAGCGGCGGTCCTTACAAGTCCGCTC520.19257832753129397No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA520.19257832753129397Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC490.18146803940448855Clontech Universal Primer Mix Long (96% over 26bp)
AAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAG480.17776461002888674No Hit
GCGGTCGGTTTCCAGTTTCGTTTCCCCGGGACCTCTTGCCCCAATTCCTC470.17406118065328494No Hit
GTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTATTA470.17406118065328494No Hit
GAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA470.17406118065328494No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT460.17035775127768313Clontech SMART CDS Primer II A (100% over 26bp)
GTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACC450.16665432190208135No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC450.16665432190208135Clontech Universal Primer Mix Long (96% over 26bp)
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC440.1629508925264795No Hit
AGGCGAGGCAGAGATGCGTAGCTGATGGGAAGCAGGTTAATATTCCTGCA430.1592474631508777No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT420.1555440337752759Clontech Universal Primer Mix Long (96% over 25bp)
AGGAATAGGTACCAAGAAAAAAACGAACCTTTGGGTTCCAGAGCTGTACG420.1555440337752759No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG420.1555440337752759Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC410.15184060439967412No Hit
CGATAGTGATGTAGAGATCTTAAATCCTGATCTTCATATCGCGACTCTTG410.15184060439967412No Hit
GGGTAAAACGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGA400.14813717502407228No Hit
GCGCTGAATCGCTTCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCAC390.14443374564847047No Hit
ACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCT390.14443374564847047No Hit
GTAGGCGAGCGTTGGTTGGTTCCTCTTGAAGCGAGAGAACCAAGGTGCTG380.1407303162728687No Hit
GAGTACATGGGGGCAAACAAGCTTTGATCCGCAGATTTCCGAATGGGGAG380.1407303162728687No Hit
ATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGTATCGGCTTCTATG380.1407303162728687No Hit
GACCTGACTGGAGTAGGTTCAAGAGAGAAAGTGGAGACAGTGATTATAGA380.1407303162728687No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC380.1407303162728687Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCC380.1407303162728687No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG380.1407303162728687No Hit
GAGTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAA370.1370268868972669No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.1370268868972669Clontech Universal Primer Mix Long (95% over 22bp)
ATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCAAGCA370.1370268868972669No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC360.13332345752166505Clontech Universal Primer Mix Long (96% over 26bp)
GACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCTACATATATAG360.13332345752166505No Hit
ACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA360.13332345752166505No Hit
AGCTGAAGGCACTCGTGGAAGAGCTTGAAGCTGATCTCCTCATCTTTAAT360.13332345752166505No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.13332345752166505No Hit
GTTTCGCCTTGACCGCGGGAAGGAGACATAACGATAGCGACTCTGTCTCA360.13332345752166505No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA350.12962002814606324Clontech SMART CDS Primer II A (96% over 26bp)
GTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAAGTATGCTGCAAACA350.12962002814606324No Hit
GGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCC350.12962002814606324No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA350.12962002814606324No Hit
CATATGCAGATCCCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCG340.12591659877046146No Hit
AAACGGAACAGGGCTGACGCCGCTACATATATAGGAAAAGGGAAGGTAGA340.12591659877046146No Hit
GTGCTGTGTTTTTATTAAACAGTCGCAGCCACCATTTTATTGCAACCCTT340.12591659877046146No Hit
TCCCAATTCTCAGAGGTCCCGCCGTACGCTGAGGACCACCTGAAACGGGC340.12591659877046146No Hit
GTGCCAGGCGTATTGCTATAAGAGGCATCTGCTCGTTTTGAGGCATAAGG340.12591659877046146No Hit
CCCAAAAGGCTGAAAGGGGGCAGTGAAGTCGGACGGACTCAACGCCCCTC340.12591659877046146No Hit
AGATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGGCG340.12591659877046146No Hit
ATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCATGTC340.12591659877046146No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG330.12221316939485966No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA330.12221316939485966No Hit
GAAGTGGTTCCAAGAAAAGCCTCTAAGCTTCAGTCTAACGAGACCGTACC330.12221316939485966No Hit
ACATGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCACA330.12221316939485966No Hit
ATTTAGGACGCACGCAGCCCTATTACGAGGGTTGTAGTCGTGTGGCACCA330.12221316939485966No Hit
ATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCATGA330.12221316939485966No Hit
GGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.11850974001925782Clontech Universal Primer Mix Long (95% over 21bp)
GTACATGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGG320.11850974001925782No Hit
GGCTGAAAGGGGGCAGTGAAGTCGGACGGACTCAACGCCCCTCTTTACAG320.11850974001925782No Hit
GACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAGGCTACCCGGATCGA320.11850974001925782No Hit
CCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATC320.11850974001925782No Hit
TAATAAGTCATAATAATCAATAATGGTTTGTGCATTTCATGTAAATCTAT320.11850974001925782No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT320.11850974001925782Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGCAGAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATG310.11480631064365603No Hit
GCGTAATTCAAGGGTGTGAGACGAGCGAACTTGTTCGCGAAGCAATCGGA310.11480631064365603No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC310.11480631064365603Clontech Universal Primer Mix Long (96% over 26bp)
CAGTAAATCAGGACGGGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGC310.11480631064365603No Hit
ATTTTATTGCATTGTATTTCATCTTACCCAACCCCTTATGAAGATGTCAA310.11480631064365603No Hit
CTTCTAGTCTCGCTTTCACTTCTTCAAGTGATTTGCGTCCTAGATTTCGA310.11480631064365603No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA300.11110288126805422Clontech SMART CDS Primer II A (96% over 26bp)
TTTCTTCCCTGACAAAAGAGCTTTACAACCCGAAGGCCTTCTTCACTCAC300.11110288126805422No Hit
AAATATAGGCTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGC300.11110288126805422No Hit
GAGTACATGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTCC300.11110288126805422No Hit
GGGTCACGCCCTGGAACTGCACGACATAACGGGTGCTACCGGCGCTGGGA300.11110288126805422No Hit
GTCTTCCGATGCAGTGTCCAGTCATGACCTCGATCCATCGCGTATAGGGA300.11110288126805422No Hit
CAGGAGGTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATG300.11110288126805422No Hit
GTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAA300.11110288126805422No Hit
TATTTAGCCTTGGAGGGTGGTCCCCCCAAATTCCCCCAAACTTGCACGTG300.11110288126805422No Hit
GTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCC300.11110288126805422No Hit
GATCGAGGTCATGACTGGACACTGCATCGGAAGACACTTCGTGCCGACAC300.11110288126805422No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG300.11110288126805422No Hit
CCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGA300.11110288126805422No Hit
GCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCAAGTTGCGAGT290.1073994518924524No Hit
TGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGATCCCGG290.1073994518924524No Hit
GGGTGAGTCGGCCCCTAAGGCGAGGCAGAGATGCGTAGCTGATGGGAAGC290.1073994518924524No Hit
CCATTTCCTTAAATTCTTCTGGGTCTGCTGACAAAGCATGATCAGGACCT290.1073994518924524No Hit
CTATCGGTCGATTACGAGTATTTAGCCTTGGAGGATGGTCCCCCCATGTT290.1073994518924524No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT290.1073994518924524Clontech Universal Primer Mix Long (96% over 26bp)
CTTTCAGCCTTTTGGGTCCTGTATCCCAATTCTCAGAGGTCCCGCCGTAC290.1073994518924524No Hit
AGGCTCTCCTTACAGGGGGTATTGTGAGATATCTCTGCACTGATCACCCA290.1073994518924524No Hit
CCGTTATTCTTTCTTCTTTCACGGTATCGGCTTCTATGGCGAATGACAGT280.1036960225168506No Hit
ATCAACGCAGAGTACATGGGGGGAATTCAGATAAAACGAATAGCTCGTAA280.1036960225168506No Hit
GAGTTGCCCCTTTGCCCGATCCCGGAAGATACGCTCTAAGCTCGGCAATC280.1036960225168506No Hit
GTCAAAACCGCGCAATTATCCCCGTCCTGATTTACTGGACTCGCAACTTG280.1036960225168506No Hit
GTACATGGGGGGAATTCGAGCTCTTTCGACGTTTTGAAGGAGGGTTTTAA280.1036960225168506No Hit
CATGGGTACCTTGCAGTTAAGATATTTAGGGCAAATAGTGGATGCCTTGG280.1036960225168506No Hit
GCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGATGC280.1036960225168506No Hit
GTACATGGGAGAGATAAAGAAAGTTTATACCTTAGACGGAAGAGAATTTA280.1036960225168506No Hit
GTATAATGTCTCGGACCCGTCGCAACGGTTGTTAATATGACAGGCCGCTA280.1036960225168506No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)