FastQCFastQC Report
Tue 10 May 2022
EGAF00003633756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003633756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230388
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA187758.14929597027623Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT131035.6873621890029Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG71223.0913068388978595Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG69443.014045870444641Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG49082.1303192874628887Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT44801.9445457228675105Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC41901.8186711113426048Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC39581.7179714221226798Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC34221.4853204159938884Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT31741.3776759206208657Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31021.3464242929319237No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG21530.9345104779762834Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC19580.8498706529853985Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA18730.8129763702970642Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC18400.7986527076062989Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT17000.7378856537666892Clontech Universal Primer Mix Long (100% over 23bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG16440.7135788322308454Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG16440.7135788322308454Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC15080.6545479799295102Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA14530.630675208778235Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC13760.5972533291664497Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC12860.558188794555272Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG12330.535184124173134Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT12260.5321457714811535Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG11670.5065367987916037Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC11390.4943833880236818Clontech Universal Primer Mix Long (96% over 25bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG10720.4653020122575829Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA10090.43795683802975843Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT9140.3967220514957376Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA8800.38196433842040384Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC8620.3741514314981683Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA7950.34507005573206934No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA7570.328576141118461Clontech SMART CDS Primer II A (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA7220.3133843776585586Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7030.30513742035175445No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT6890.29906071496779346Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC6600.28647325381530286Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG5980.2595621299720472Clontech Universal Primer Mix Long (96% over 26bp)
GCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG5680.24654061843498795Clontech Universal Primer Mix Long (96% over 26bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT5530.24002986266645834Clontech SMART CDS Primer II A (96% over 26bp)
GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA5340.23178290535965418Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5200.22570619997569316No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA5000.21702519228432035Clontech SMART CDS Primer II A (96% over 26bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC4830.20964633574665348Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT4590.1992291265170061Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA4540.19705887459416288Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT4340.18837786690279007Clontech Universal Primer Mix Long (96% over 25bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC4300.1866416653645155Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC4280.18577356459537822Clontech Universal Primer Mix Long (96% over 25bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG4210.18273521190339775Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG4110.17839470805771135Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA3850.16710939805892666No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT3720.16146674305953435Clontech SMART CDS Primer II A (100% over 26bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA3700.16059864229039708Clontech Universal Primer Mix Long (96% over 26bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC3670.15929649113669114Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACTGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG3650.15842839036755385Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3640.15799433998298523No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA3510.1523516849835929Clontech Universal Primer Mix Long (96% over 26bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA3430.14887928190704378Clontech Universal Primer Mix Long (96% over 26bp)
GTATAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG3310.14367067729222008Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC2970.1289129642168863Clontech SMART CDS Primer II A (96% over 26bp)
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA2850.12370435960206261Clontech Universal Primer Mix Long (96% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2800.1215341076792194Clontech Universal Primer Mix Long (96% over 26bp)
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG2770.1202319565255135Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG2720.11806170460267028Clontech SMART CDS Primer II A (96% over 26bp)
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG2700.11719360383353299Clontech Universal Primer Mix Long (96% over 26bp)
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA2690.11675955344896435Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2630.1141552511415525Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT2420.10504019306561106Clontech SMART CDS Primer II A (100% over 26bp)
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA2390.10373804191190514No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG2340.10156778998906192Clontech Universal Primer Mix Long (96% over 25bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA2320.10069968921992466Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)