FastQCFastQC Report
Tue 10 May 2022
EGAF00003633928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003633928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362277
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA274807.585355956905904Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT126713.4975999028367797Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG104292.878736436483685Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG99602.7492774865641483Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG91712.531488336273073No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG71551.9750080739323777Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC54271.4980249919260675Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT45401.253184717771208Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT45121.2454558252386985Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC43371.197150246910513Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC36941.0196617505389522Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT26540.7325885993314508Clontech Universal Primer Mix Long (100% over 23bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG24550.6776582559754001Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA24210.6682731721859241No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA24190.6677211084336019Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG23570.6506071321116162Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG22410.6185874344769334Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC21550.5948486931270823Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG21180.5846355137091231Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG20070.5539959754552456Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC17780.4907846758143631Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC15830.4369584599629565Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA15630.4314378224397354Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC14990.41377178236542755Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC14820.40907924047068955Clontech Universal Primer Mix Long (96% over 25bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC13980.3858925628731606Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG13910.3839603397400332Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA13420.37043477780814127Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC13350.36850255467501386Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12200.3367588889164921Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT11530.3182647532137011Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11420.3152284025759295No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA10530.2906615655975952No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC10240.2826566411889245Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA9460.2611261548483619Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA8660.23904360475547715No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7400.20426358835918373No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT7290.2012272377214121Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT7120.19653469582667404Clontech SMART CDS Primer II A (100% over 26bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC7000.19322231331274134Clontech Universal Primer Mix Long (96% over 26bp)
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT6870.1896338989226476No Hit
GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA6800.18770167578952016Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA6370.17583230511459463No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA6170.17031166759137345Clontech Universal Primer Mix Long (96% over 25bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC6100.16837944445824604Clontech Universal Primer Mix Long (96% over 25bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT6090.16810341258208497Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA6080.16782738070592393Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5770.15927039254493108No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA5760.15899436066877Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT5730.15816626504028686Clontech SMART CDS Primer II A (100% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG5630.15540594627867627Clontech Universal Primer Mix Long (96% over 26bp)
GCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG5570.15374975502170993Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC5560.15347372314554886Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC5510.15209356376474353No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA5090.14050022496597908Clontech SMART CDS Primer II A (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT5080.140224193089818Clontech Universal Primer Mix Long (96% over 26bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA4810.13277133243346942Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG4810.13277133243346942Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC4740.13083910930034198No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA4500.1242143442724766No Hit
CCACTGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG4420.12200608926318812Clontech Universal Primer Mix Long (96% over 26bp)
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA4360.12034989800622177Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT4250.11731354736845012Clontech Universal Primer Mix Long (96% over 25bp)
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG4110.11344910110219528Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA4090.11289703734987316No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA4060.11206894172139Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC4000.11041275046442363No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA4000.11041275046442363No Hit
GAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA3770.10406401731271928No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAA3760.10378798543655822No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3680.10157973042726974No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA3680.10157973042726974Clontech Universal Primer Mix Long (95% over 21bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)