FastQCFastQC Report
Tue 10 May 2022
EGAF00003634137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003634137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177177
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG7020.3962139555359894No Hit
GTACATGGGAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGC6570.3708156250529132No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4800.2709155251528133No Hit
GGAACAGGTTGAACAGTCTACCCTCCCTTAGCAGGGAACTACTCCCACCC4330.24438837998160035No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4120.23253582575616474Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT4110.23197141841209637No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT4000.2257629376273444No Hit
GTACATGGGCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAAC3920.22124767887479752No Hit
GAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGT3830.2161680127781823No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT3140.17722390603746535No Hit
CATGGGAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTT2980.1681933885323716No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC2770.15634083430693602No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2700.15238998289845748No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT2640.1490035388340473No Hit
CTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCC2520.14223065070522697No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT2520.14223065070522697No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC2430.13715098460861172No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG2240.12642724507131287No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA2220.12529843038317615No Hit
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC2210.1247340230391078No Hit
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT2210.1247340230391078No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2190.12360520835097107Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG2170.12247639366283435No Hit
TATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATATCATAG2170.12247639366283435No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT2160.12191198631876599No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2130.1202187642865609No Hit
GTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGCCACCTACGGTGA2070.11683232022215073No Hit
GCCATAACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCAC1980.11175265412553548No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC1970.11118824678146712No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT1960.11062383943739876No Hit
GTATCAACGCAGAGTACATGGGAGCCCCTCTCCGGCCCCGGCCGGGGGGC1920.1083662100611253No Hit
ATATAAAACCCCCTGCCATAACCCAATACCAAACGCCCCTCTTCGTCTGA1910.10780180271705697No Hit
CTCCTACTCCTGCTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGG1860.10497976599671516No Hit
CACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCAC1820.1027221366204417No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT1820.1027221366204417No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTTACCTATGATCTCGTATG1810.10215772927637334No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC1800.10159332193230498No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA1780.10046450724416826No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)