FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003634274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003634274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62
Sequences flagged as poor quality0
Sequence length100
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCCAGGTCGCGATCGACAAGGCCGCGAGCCGTGCCGGCAAGCGGGCGT34.838709677419355No Hit
GACCGTGGTCGGTGACGGCGATGGCCGCGTCGGCATGGGCAAGGGCAAGT34.838709677419355No Hit
GTGTAGGATCAATTGTAGAAAAAAATTGTATCCATAAACACTTCAATAAG34.838709677419355No Hit
GTTTGAGGACGGTCACTTCGACCTCCTCACCTTGGTCAGATCGAAACTCA34.838709677419355No Hit
TCTGCGTCCCGATCGTACCGCCGGCGCTCCGGGTCGCGAGGCATGGTGCG23.225806451612903No Hit
GTCATAGAAAGTGTCTGACGGGTTCTATGACCATGAGGACACCACCACTG23.225806451612903No Hit
GAGCGGCGGCAGGCGTCGTAGCCCAGGTTCACCGTCTGTCTGCTGGGGCT23.225806451612903No Hit
AGATTGGAGACTATTATTCTAACTGAAGTAACTCAGGAATGGAAAACCAA23.225806451612903No Hit
AGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGT23.225806451612903No Hit
TCCGCCGGAGTCCGGTCGCGGTCAGGCGCGACCGGAAGGCGCCGACCACG23.225806451612903No Hit
GAGTGGGTAAAGAGAGAATGTGTTTTGTAAAATGAAATTCAAGTAAATTT23.225806451612903No Hit
CTGTAAACGAATGAAAATAGCGGGAACGCCGGTAAAGTAACCCTCGCCTT23.225806451612903No Hit
GCTGTTGCATGTAACCTTGAAGAAGAATAAAAAAACAAACAAAAAACATT11.6129032258064515No Hit
GTGTACCTCATTGTTACTCACTATGTTTTTCTTGAGAACCTGCTCCCTTC11.6129032258064515No Hit
GAATGAGAGGTCTCCTGCGGTAGACTCGAGGTCGCTGGTCAGTGCTTTGA11.6129032258064515No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA11.6129032258064515No Hit
GCCCGGGATGAGACCCTGCAGCTTGCCCGAGGCGATGAGCGATCCGGTGA11.6129032258064515No Hit
AGTCCAATGCGACGACAGCGCCCTTCTTTCCCTTGAATCCGTCCCACGCG11.6129032258064515No Hit
TTCCCACTCTAAGTGTCCTACTTCCATCTTCTTCCCATCTCTTTGTCTCT11.6129032258064515No Hit
TTGCCAGCATGTGCAACAGCACCGGAACCGCAACATTATACGGCAAGTTC11.6129032258064515No Hit
AGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGC11.6129032258064515No Hit
GTTGTGGATCGTCGGCCCAACCGTTGGTGAGATAGTTATACATCTGGGTG11.6129032258064515No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT11.6129032258064515Clontech Universal Primer Mix Long (96% over 26bp)
TTAATAGAAAGTGTCTGACGGGTTCTATGACCATGAGGACACCACCACTG11.6129032258064515No Hit
GGGCGGAGGCGACATGCCGGCGACGATCATCATGGGCGGCCAGTGGGGTG11.6129032258064515No Hit
GGATCACGCGGTTGGTCGCCCCCCCCATCAAAACCGCCGTTGGGGGGAGG11.6129032258064515No Hit
GGCCAGCACAGTGGCTCATACCTGTAATCCCAGCACTTTGGGAGGCTGAG11.6129032258064515No Hit
CAAGTATGGCCCGTTGCGCTTGCCCCGGCGCGGGGCGGGAGTTAGGAAGG11.6129032258064515No Hit
CCACCAGGGTCGGCTCGCGACCCGGTCCGACCCCGACGCCCCAGATCATC11.6129032258064515No Hit
GTCGCGAACGTGTTCGCCGGGTTGACGACGACGGTCAGCCCGTCGATCGC11.6129032258064515No Hit
CATCACACCTGGCTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.6129032258064515No Hit
GTCGGGGTGCTCGCGCACCTCGACGGACCGGGCGAGGGCGACCCCGCCCT11.6129032258064515No Hit
CCACGGTTTTTTAATGAGAAACATGGTTGCAAGTCCAAATAATCCAGCGA11.6129032258064515No Hit
CTCTTCGCACACCGTCGGGCCCATGCGCGCGGCTAAGCAATTCCTCGATT11.6129032258064515No Hit
GAAGAGCTGGTTGCCGTGCTTTTCGGTGGCCAGGGTGGAGTTCGGGCCGG11.6129032258064515No Hit
GCCTAAGGAAATGCAGGGAAAAGTAAGAGCAGAAGGATGACTCCTCACGC11.6129032258064515No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.6129032258064515No Hit
CACCCATGTCTCGGTGGCTGCGGGCCGGGGGCCGACGTCGCAGTGGAGTA11.6129032258064515No Hit
GCGTCGCGGCGTCCCGCTCGCCAGGCGGATCGCCGCATCGATCGCCGACA11.6129032258064515No Hit
GGATTTTATAGAGGGGACTGATTATGGCCAGGATTCCTAAAGGGGCAGAG11.6129032258064515No Hit
CCCTCTGCCTGTGTCTCTGCCTCTCTCTCTCTCTCTCTCTCTCTCTCTGT11.6129032258064515No Hit
CTCGTGAGCCCCACAGCCGCTCTAGTCCGGGAACATCTGGCAATTCGTCA11.6129032258064515No Hit
GCCGACTCGAGATCGACGTTGTCGACGCCGACGCCCGCCCGGCCGATGAC11.6129032258064515No Hit
GGGTTGGTGCAGTAGCGGGCGGCCTCGCCCTCTCGCCGCTCGGTCGGCCA11.6129032258064515No Hit
GTCCAACACCTTTGGCGCAAACTCCGGAGCCTCGTCGAGAAACAACACCC11.6129032258064515No Hit
CAGCATGAAGTCCACCCAGAAGTTGCCGAGGTCGTGCCGCCCGCTGCGGG11.6129032258064515No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)