FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003634357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003634357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4636
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.45297670405522No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG160.3451251078515962No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA110.2372735116479724No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAA80.1725625539257981No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG80.1725625539257981No Hit
TCCTTGTTCCGGTCCAAGTCTTCCATCAGCCTTGCAATTTCAGCATCCTG70.15099223468507333No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA70.15099223468507333Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCCCAGAGGGTGTCTCCATCTTCCCTATTTATTTTCAGCCCTTGAGGGC60.12942191544434856No Hit
GTTTAAATGACTGTGCTGCCCCTTTCACATCAAAGAACTACTGACAACGA60.12942191544434856No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT60.12942191544434856No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG60.12942191544434856No Hit
GTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTTTTAGCTGTTTAA60.12942191544434856No Hit
CCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC60.12942191544434856No Hit
GGGATGCAAACAATACAAAAATCAAAAGCTTATCTGGTATTTAACTTTTC60.12942191544434856No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA60.12942191544434856No Hit
GACACTGGCTGTCCACCTGGCACTGGTCCCGACAGAGGCCGAGCTGGGGA50.10785159620362382No Hit
AGGTAACGGGGCAGAGATGTGGTTCGAGATTCTCCCCGGACTCTCCGTCA50.10785159620362382No Hit
GAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG50.10785159620362382No Hit
CCTCTCCAGAAGAGGAGAAGAGGAAACACAAGAAGAAACGCCTGGTGCAG50.10785159620362382No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCT50.10785159620362382No Hit
CTCTTGGAGCCTCGGCTGAAGTGGCTGGGCATGACGCCGTTTCTCTGACG50.10785159620362382No Hit
GACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAACCATG50.10785159620362382No Hit
GTACATGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCA50.10785159620362382No Hit
GATAGACAGATGGGAAAGGCAGGCGCGGCCTTCGTTGTCAGTAGTTCTTT50.10785159620362382No Hit
TCCTCAAAAAGTCCGGGAAGCTGAAAGTCCCCGAATGGGTGGATACCGTC50.10785159620362382No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGC50.10785159620362382No Hit
GCCACACACTTGTGGAGCTAGAAATAGTGGGGCAGGTCCTTCTCTATCAC50.10785159620362382No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCC50.10785159620362382No Hit
TGTCTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAA50.10785159620362382No Hit
TTCCACAAGCATTGCCTTCTTATTTTACTTCTTTTAGCTGTTTAACTTTG50.10785159620362382No Hit
CGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCC50.10785159620362382No Hit
CCTCCGCAACCATGTCTGACAAACCCGATATGGCTGAGATCGAGAAATTC50.10785159620362382No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC50.10785159620362382No Hit
GGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTT50.10785159620362382No Hit
CCTCCCTACCGCTCCAAGCCCAGCCCTCAGCCATGGCATGCCCCCTGGAT50.10785159620362382No Hit
GCTATAACAGCGAGTGCTACGCCTAAACTGGCTGTCTAGCACTGTAGCTG50.10785159620362382No Hit
CTTTGTCCCTGAGGTGTCAGTTTGCGGCCGCCATCTTGGTCCTTTTCCAC50.10785159620362382No Hit
GTTTTTAAACTTTTTATTTGCATATTAAAAAAATTGTGCATTCCAATAAT50.10785159620362382No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)