FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003634358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003634358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4636
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA100.21570319240724764No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA90.19413287316652286No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.19413287316652286No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.1725625539257981No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC70.15099223468507333No Hit
CTCTTGGAGCCTCGGCTGAAGTGGCTGGGCATGACGCCGTTTCTCTGACG70.15099223468507333No Hit
CCTCCGCAACCATGTCTGACAAACCCGATATGGCTGAGATCGAGAAATTC70.15099223468507333No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.15099223468507333No Hit
GCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGATT60.12942191544434856No Hit
GTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCC60.12942191544434856No Hit
GCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCC60.12942191544434856No Hit
GTGTAGATCAGAGCATCGGACAGGTACACAGATACGCGCTCAGCATAGCA60.12942191544434856No Hit
GTGCGGGACAGCAAGCCCCCGAATAGCCCCGGCTGCCACCTCGCAGGACC60.12942191544434856No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG60.12942191544434856No Hit
GTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCG60.12942191544434856No Hit
CTGCAGGCCTACGTGCAGCGGCTGAAGGAGTACCGCTCCAAACTCATCCT50.10785159620362382No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT50.10785159620362382No Hit
CACCATGCCTGCTTGTCGCCTAGGCCCGCTAGCCGCCGCCCTCCTCCTCA50.10785159620362382No Hit
TGCCTGGAGTTACTGTAAAAGACGTGAACCAGCAGGAGTTCGTCAGAGCT50.10785159620362382No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT50.10785159620362382No Hit
GTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCT50.10785159620362382No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC50.10785159620362382Clontech Universal Primer Mix Long (96% over 26bp)
TGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAA50.10785159620362382No Hit
GCTTGGTTTTACTCTAGATTTCACTGTCGTCCCACCCCACTTCTTCCACC50.10785159620362382No Hit
GGCCTTCCCGTGTAACCAGTTCGGGAAGCAGGAGCCAGGGAGTAACGAAG50.10785159620362382No Hit
GGCCAAGCACAAAGAGCTTGCTCCCTACGATGAGAACTGGTTCTACACGC50.10785159620362382No Hit
GTACATGGGAGTCTCGCGCCTGCAGCCCGTGCCGCCCCAGCCGCTGCCGC50.10785159620362382No Hit
GGCAAGATTCTTGCCAAGAGAATTAATGTGCGTATTGAGCACATTAAGCA50.10785159620362382No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA50.10785159620362382No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG50.10785159620362382No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)