FastQCFastQC Report
Wed 11 May 2022
EGAF00003634435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003634435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4690754
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAGGACTGTTGCCTCAAGTACAGCCAAAGGAAGATTCCCGCCAAGGT93020.19830500597558517No Hit
AGCTTAGGCTGCTCCATCCCAGCTATCCTGTTCTTGCCCCGCAAGCGCTC75990.16199954207788342No Hit
GGGTAGAGCTGGGAATGCAGATGGGGTGGTTAAAGCAGGAGAAAGAGTGT73630.1569683679851896No Hit
GTCCTGCTGCCTCCTCTCATGCTCCCTGGGAGACTCTCCCTCCTCGGTCT72320.1541756399930587No Hit
CTCTGGACCTGGCCTGCTGTGGGCAGCTCAGCCATGCAGGGTAGAGCTGG70230.1497200663262239No Hit
TCCTTATCCTGGTTCTGGCCTTTGGCATCCCCAGGACCCAAGGCAGTGAT66360.14146979355557765No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG64120.1366944418743767No Hit
GTACTGGGGAGCCGTATCAGGTCCAGGGTCCTGATGATTCTCCTTCAAGG63140.1346052255138513No Hit
GCTATGTGCAGACCCAAAGGAGCTCTGGGTGCAGCAGCTGATGCAGCATC60500.12897713246100734No Hit
CTTTAGGGGTCTGTGACCGCTCAGTCCTCTTGCAGCCTTTGGAGCCCTTT50150.10691244946974411No Hit
CCCCAGGACCCAAGGCAGTGATGGAGGGGCTCAGGACTGTTGCCTCAAGT49290.1050790555207116No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)