FastQCFastQC Report
Tue 10 May 2022
EGAF00003634478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003634478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5647
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG140.24791924915884542No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG130.23021073136178502No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG120.21250221356472462No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGG100.17708517797060386No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC100.17708517797060386No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT90.1593766601735435No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG90.1593766601735435No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG90.1593766601735435No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA80.1416681423764831No Hit
GCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACAC80.1416681423764831No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT80.1416681423764831No Hit
CCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAG80.1416681423764831No Hit
GGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGA80.1416681423764831No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG80.1416681423764831No Hit
CCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCG70.12395962457942271No Hit
CCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTC70.12395962457942271No Hit
CTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTC70.12395962457942271No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA70.12395962457942271No Hit
GTCTTGATCAGGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGAGGGTG70.12395962457942271No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT70.12395962457942271No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT70.12395962457942271No Hit
CGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCA60.10625110678236231No Hit
ACCAGAGACAGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTC60.10625110678236231No Hit
TCGCGGCGGACCGCCCGCCCGCTCCCAAGATCCAACTACGAGCTTTTTAA60.10625110678236231No Hit
GTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGA60.10625110678236231No Hit
GTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCAT60.10625110678236231No Hit
GATCAAGACATGAGGGAGGCAGGGGCTCAGCTGAAGAAGCTGGTGGACAC60.10625110678236231No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT60.10625110678236231No Hit
GGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGA60.10625110678236231No Hit
GCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTC60.10625110678236231No Hit
CCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAG60.10625110678236231No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)