FastQCFastQC Report
Tue 10 May 2022
EGAF00003634729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003634729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences585599
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT20610.3519473223144165No Hit
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCC16420.28039665368281025No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT15030.2566602743515614No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC14260.2435113447939631No Hit
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC13690.23377772161496177No Hit
GGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTG12680.21653042440304715No Hit
GAATATGATAGTGAAATGGATTTTGGCGTAGGTTTGGTCTAGGGTGTAGC12260.2093582810079935No Hit
GTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGC12220.20867521973227413No Hit
CACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCAC12120.20696756654297566No Hit
CTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCC12110.2067968012240458No Hit
ATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCC11960.20423532144009807No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA11590.19791700463969372No Hit
TATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATATCATAG10780.18408501380637604No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT10680.18237736061707754No Hit
GTACATGGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAAT10650.181865064660288No Hit
GTCCTAATCACAGCAGTCCTACTTCTCCTATCTCTCCCAGTCCTAGCTGC10630.18152353402242832No Hit
GTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGCCACCTACGGTGA10480.1789620542384806No Hit
CCTATTGATAGGACATAGTGGAAGTGAGCTACAACGTAGTACGTGTCGTG10250.17503445190309408No Hit
GGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAATGAAGCCTCCT10220.17452215594630455No Hit
CTCCTACTCCTGCTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGG10070.17196067616235683No Hit
GTCTACCCTCCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGA10020.1711068495677076No Hit
ATATAAAACCCCCTGCCATAACCCAATACCAAACGCCCCTCTTCGTCTGA9970.17025302297305836No Hit
ACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCACAGCAGT9920.1693991963784091No Hit
CTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTTCGAAG9680.16530082872409277No Hit
TAGTGGAAGTGAGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAG9660.16495929808623305No Hit
GTCCTAGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCAACAC9430.16103169575084655No Hit
ACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGC9250.1579579200101093No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG8970.15317649108007358No Hit
TCACTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCC8860.15129807257184524No Hit
GTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTC8860.15129807257184524No Hit
CCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCACCT8750.1494196540636169No Hit
GAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATC8690.1483950621500378No Hit
GCCTACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCG8660.14788276619324828No Hit
GGGTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAAT8660.14788276619324828No Hit
CCTTACACCTAGCAGGTGTCTCCTCTATCTTAGGGGCCATCAATTTCATC8590.14668740896073934No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC8500.14515052109037072No Hit
GTAAATATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATAT8320.14207674534963347No Hit
GTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTT8240.14071062279819466No Hit
GTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTGTTGA8240.14071062279819466No Hit
GTAATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGACGGATCGGAGAATT7870.13439230599779028No Hit
GTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTAT7830.1337092447220709No Hit
GGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGAGCTACAACGT7750.13234312217063213No Hit
ATTCCGGATAGGCCGAGAAAGTGTTGTGGGAAGAAAGTTAGATTTACGCC7740.13217235685170226No Hit
GACCATACCTATGTATCCAAATGGTTCTTTTTTTCCGGAGTAGTAAGTTA7710.13166006089491272No Hit
CATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCCGAGAAAG7610.12995240770561425No Hit
TAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTA7550.12892781579203516No Hit
GGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCAT7500.12807398919738594No Hit
GCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCT7490.12790322387845607No Hit
ACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAG7490.12790322387845607No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7380.12602480537022775Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATC7360.12568327473236807No Hit
TCACTAGACATCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCA7270.12414638686199943No Hit
GGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTGAGCAC7200.12295102962949049No Hit
CATAGGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCG7180.12260949899163079No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA7090.12107261112126216No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTACTTGTCCA7070.12073108048340246No Hit
GACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTAT7060.12056031516447263No Hit
GTTTATATTCTTATCCTACCAGGCTTCGGAATAATCTCCCATATTGTAAC7020.11987725388875323No Hit
CCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCAT7000.11953572325089352No Hit
TTCATGTGGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCG6980.11919419261303384No Hit
GCCATAACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCAC6950.1186818966562443No Hit
CTCCACGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCTAGG6920.11816960069945474No Hit
GGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTTCGAAGCGAAG6890.1176573047426652No Hit
TCCGTGGAGTGTGGCGAGTCAGCTAAATACTTTGACGCCGGTGGGGATAG6860.11714500878587567No Hit
GTCCTACTTCTCCTATCTCTCCCAGTCCTAGCTGCTGGCATCACTATACT6800.11612041687229659No Hit
TCCCCACCCCATCCAACATCTCCGCATGATGAAACTTCGGCTCACTCCTT6780.11577888623443687No Hit
GAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTGAGCACACCAT6740.11509582495871748No Hit
GTCTACGTCTATTCCTACTGTAAATATATGGTGTGCTCACACGATAAACC6660.1137297024072787No Hit
GATTATGGTAGCGGAGGTGAAATATGCTCGTGTGTCTACGTCTATTCCTA6530.11150975326119068No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG6530.11150975326119068No Hit
GGACTATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTC6530.11150975326119068No Hit
TGATAGGACATAGTGGAAGTGAGCTACAACGTAGTACGTGTCGTGTAGTA6520.11133898794226083No Hit
GTCCCAATGTATGGGATGGCGGATAGTAAGTTTGTAATTACTGTGGCCCC6460.11031439602868175No Hit
TCCTAGGGTTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGAC6440.10997286539082204No Hit
GGTGGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTAC6380.10894827347724297No Hit
GTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCCGAGGGCGTCT6320.10792368156366386No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCCGTAAGTAGGAGAGTGATAT6280.10724062028794447No Hit
CTCCTATGATGGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGA6260.10689908965008478No Hit
CTTCTACTATTAGGACTTTTCGCTTCGAAGCGAAGGCTTCTCAAATCATG6150.10502067114185647No Hit
GTTTAAGGGGTTGGCTAGGGTATAATTGTCTGGGTCGCCTAGGAGGTCTG6130.10467914050399675No Hit
CATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACA6120.10450837518506692No Hit
CATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTTTT6110.10433760986613706No Hit
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT6100.10416684454720722No Hit
CCTCTATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAA6090.10399607922827736No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC6040.10314225263362814No Hit
GGTGTAGCCTGAGAATAGGGGAAATCAGTGAATGAAGCCTCCTATGATGG6040.10314225263362814No Hit
CCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTC6030.10297148731469828No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT5980.10211766072004903No Hit
CATCTACAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCA5950.1016053647632595No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)