FastQCFastQC Report
Tue 10 May 2022
EGAF00003634730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003634730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences585599
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT18500.3159158400202186No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT14800.2527326720161749No Hit
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC14470.2470974164914899No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC14250.24334057947503324No Hit
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCC13060.22301950652238137No Hit
CACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCAC12850.21943343482485456No Hit
CTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCC12800.2185796082302053No Hit
GAATATGATAGTGAAATGGATTTTGGCGTAGGTTTGGTCTAGGGTGTAGC12430.21226129142980096No Hit
GGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTG12030.20543067867260703No Hit
ATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCC11320.19330634102858782No Hit
GTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGC10970.18732955486604314No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA10800.18442654444423573No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT10740.18340195253065664No Hit
ACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCACAGCAGT10690.18254812593600742No Hit
GTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGCCACCTACGGTGA10310.1760590438166732No Hit
GTCCTAATCACAGCAGTCCTACTTCTCCTATCTCTCCCAGTCCTAGCTGC9960.17008225765412852No Hit
TATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATATCATAG9880.1687161351026897No Hit
GTACATGGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAAT9230.15761638937224962No Hit
ATATAAAACCCCCTGCCATAACCCAATACCAAACGCCCCTCTTCGTCTGA9190.1569333280965302No Hit
CCTATTGATAGGACATAGTGGAAGTGAGCTACAACGTAGTACGTGTCGTG9130.15590873618295112No Hit
TCACTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCC9110.15556720554509143No Hit
TAGTGGAAGTGAGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAG8900.15198113384756462No Hit
CTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTTCGAAG8740.14924888874468706No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG8700.14856582746896768No Hit
GGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAATGAAGCCTCCT8670.14805353151217815No Hit
GGGTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAAT8610.14702893959859903No Hit
CTCCTACTCCTGCTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGG8570.14634587832287965No Hit
GTAAATATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATAT8330.1422475106685633No Hit
GTCCTAGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCAACAC8300.14173521471177375No Hit
GAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATC8290.1415644493928439No Hit
ACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGC8280.14139368407391406No Hit
GCCTACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCG8270.14122291875498422No Hit
GTCTACCCTCCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGA8130.13883220428996634No Hit
GTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTAT8110.13849067365210666No Hit
GTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTT8030.13712455110066787No Hit
CCTTACACCTAGCAGGTGTCTCCTCTATCTTAGGGGCCATCAATTTCATC8020.136953785781738No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC7890.13473383663564997No Hit
GACCATACCTATGTATCCAAATGGTTCTTTTTTTCCGGAGTAGTAAGTTA7880.13456307131672016No Hit
GGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTGAGCAC7800.13319694876528138No Hit
CCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCACCT7690.13131853025705303No Hit
GGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGAGCTACAACGT7590.12961087706775454No Hit
GCCATAACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCAC7350.12551250941343822No Hit
GTACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATC7300.12465868281878896No Hit
ATTCCGGATAGGCCGAGAAAGTGTTGTGGGAAGAAAGTTAGATTTACGCC7250.12380485622413974No Hit
GTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTC7210.12312179494842035No Hit
TCCTAGGGTTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGAC7170.12243873367270094No Hit
ACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAG7140.12192643771591138No Hit
GTAATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGACGGATCGGAGAATT7120.12158490707805171No Hit
GTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTGTTGA7070.12073108048340246No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA7050.12038954984554277No Hit
TCACTAGACATCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCA7000.11953572325089352No Hit
GGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCAT6940.11851113133731445No Hit
CATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCCGAGAAAG6920.11816960069945474No Hit
GTCCTACTTCTCCTATCTCTCCCAGTCCTAGCTGCTGGCATCACTATACT6810.11629118219122642No Hit
CATAGGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCG6730.11492505963978762No Hit
GAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTGAGCACACCAT6730.11492505963978762No Hit
GCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCT6690.11424199836406823No Hit
TAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTA6670.11390046772620854No Hit
CCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCAT6670.11390046772620854No Hit
GTTTATATTCTTATCCTACCAGGCTTCGGAATAATCTCCCATATTGTAAC6560.11202204921798023No Hit
GGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTTCGAAGCGAAG6530.11150975326119068No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTACTTGTCCA6530.11150975326119068No Hit
GACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTAT6500.11099745730440115No Hit
CATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACA6440.10997286539082204No Hit
TTCATGTGGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCG6340.10826521220152358No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG6340.10826521220152358No Hit
TCCGTGGAGTGTGGCGAGTCAGCTAAATACTTTGACGCCGGTGGGGATAG6310.10775291624473403No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC6280.10724062028794447No Hit
CTCCACGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCTAGG6230.10638679369329525No Hit
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT6190.10570373241757586No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6130.10467914050399675Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATTATGGTAGCGGAGGTGAAATATGCTCGTGTGTCTACGTCTATTCCTA6120.10450837518506692No Hit
CTTCTACTATTAGGACTTTTCGCTTCGAAGCGAAGGCTTCTCAAATCATG6030.10297148731469828No Hit
GTCTACGTCTATTCCTACTGTAAATATATGGTGTGCTCACACGATAAACC6020.10280072199576842No Hit
ATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTC5970.1019468954011192No Hit
GTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCCGAGGGCGTCT5910.1009223034875401No Hit
GTCCCAATGTATGGGATGGCGGATAGTAAGTTTGTAATTACTGTGGCCCC5870.1002392422118207No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)