FastQCFastQC Report
Tue 10 May 2022
EGAF00003634859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003634859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30107
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGAGCGGTATCTATCTCGTATG1990.6609758527917096No Hit
CTTATACACATCTCCGAGCCCACGAGACAGAGCGGTATCTATCGCGTATG1520.504865978011758No Hit
ATACACATCTCCGAGCCCACGAGACAGAGCGGTATCTATCTCGTATGCCG1250.4151858371807221No Hit
ATACACATCTCCGAGCCCACGAGACAGAGCGGTATCTATCGCGTATGCCG1100.36536353671903543No Hit
ACACATCTCCGAGCCCACGAGACAGAGCGGTATCTATCTCGTATGCCGTC800.26571893579566214No Hit
ACACATCTCCGAGCCCACGAGACAGAGCGGTATCTATCGCGTATGCCGTC720.23914704221609592No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT700.2325040688212044No Hit
CACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCT530.17603879496462616No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC430.1428239279901684No Hit
GCTGCATACTGCAGCCTGTCTGCAAACCAATGAGGAGTGGGACAGCATGG430.1428239279901684No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.13950244129272263No Hit
CATCTCCGAGCCCACGAGACAGAGCGGTATCTATCTCGTATGCCGTCTTC390.1295379812003853RNA PCR Primer, Index 38 (95% over 23bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA390.1295379812003853No Hit
ATACCAAGACGTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCAC380.1262164945029395No Hit
ACTTGAACCGTGGCGACTGCGGTGGACTCTGGTTGGTTCGCCTGAATCGG380.1262164945029395No Hit
GTGCATACCTCCAGGTTGCTGGCCTGCGAAGTTCTCATTCCGGGGGGTAA360.11957352110804796No Hit
CTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGA350.1162520344106022No Hit
GTCAAAACCGCGCAATTATCCCCGTCCTGATTTACTGGACTCGCAACGTG350.1162520344106022No Hit
CTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGAAT350.1162520344106022No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT340.1129305477131564No Hit
GCTCAAGGAACTGCTCGACAAGGGCCTCATCACGCAAGCGGACTTTGATG330.10960906101571063No Hit
GTACATGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTCCGC330.10960906101571063No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT330.10960906101571063Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT320.10628757431826484Clontech SMART CDS Primer II A (100% over 26bp)
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA310.10296608762081907No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA310.10296608762081907No Hit
GTACATGGGCAAGCAGAAGACGGCATACGAGATCTTGCTTCGTGACTGGA310.10296608762081907Illumina PCR Primer Index 1 (96% over 28bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)