FastQCFastQC Report
Tue 10 May 2022
EGAF00003634860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003634860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30107
Sequences flagged as poor quality0
Sequence length100
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19276.400504865978012No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT890.29561231607267413No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT880.2922908293752283No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT550.18268176835951772No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA400.13285946789783107No Hit
GTGGTAGCCAACGCGAATCCGGCAGAAGACGGCGTGTTGGTCGAGCTCGG390.1295379812003853No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT380.1262164945029395Clontech SMART CDS Primer II A (100% over 26bp)
GATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGA380.1262164945029395No Hit
GATTTCCACTCAGCGGCGCCTGGCCCCGCCATCACATTGAGGTAGTACAA350.1162520344106022No Hit
CGCGTAGTCCTCGAGCACGGCAGACATCTTGCCGATGTCGGAAAGCGTCT350.1162520344106022No Hit
GTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACCAGTACTGA350.1162520344106022No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG330.10960906101571063Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.10960906101571063No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA330.10960906101571063No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT310.10296608762081907Clontech SMART CDS Primer II A (100% over 26bp)
GCGCAGGGGGTTCTGCGGGCGCTGCCCGGCTGCCCGTCTTGCTCCCGCGA310.10296608762081907No Hit
TATCAACGCAGAGTACATGGGGGGAATTCCTGGAGATTGTCTCGTACGGT310.10296608762081907No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)