FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003634969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003634969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA88.791208791208792Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC55.4945054945054945Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG33.296703296703297Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT33.296703296703297Clontech Universal Primer Mix Long (96% over 26bp)
TGTTTATTGGAAGCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGC22.197802197802198No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG22.197802197802198No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT22.197802197802198Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG22.197802197802198Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG22.197802197802198Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC22.197802197802198Clontech Universal Primer Mix Long (96% over 26bp)
TCTGTACCCAGTGAAGAGGCGCTTGTCTTGCTGTAGCAACTCGGTTCCTC22.197802197802198No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA22.197802197802198Clontech Universal Primer Mix Long (96% over 26bp)
ATATCAGCCTCTTCCCCCTGGACGATGTGCAGCCTTCGAAGTTGATGCGA22.197802197802198No Hit
GGCCTACCCTGCTCTGTCCGTGGATGAGGCCACCACAGCTATGCAGGGCA22.197802197802198No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC22.197802197802198Clontech Universal Primer Mix Long (96% over 26bp)
CCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGA11.098901098901099No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT11.098901098901099Clontech Universal Primer Mix Long (100% over 23bp)
TGCTTGAACTTGGTGGCACTGGCAATGTTGGGGAAGGTGAAGAAGGCCAG11.098901098901099No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG11.098901098901099Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACAATGCGTTGATACC11.098901098901099No Hit
ACGCAGAGTACATGGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGAA11.098901098901099Clontech Universal Primer Mix Long (96% over 27bp)
GTACTCTGCGTTGATACACTGCTTCCCATGTACTCTGCGTTGATACCACT11.098901098901099Clontech SMART CDS Primer II A (95% over 22bp)
ACGCAGAGTACATGGGCAAGCAGTGGTATCAACGCTGTCTCTTATACACA11.098901098901099Clontech Universal Primer Mix Long (95% over 24bp)
GGTGTTCAGCGAAGCCGAGCTGGCCGCGATGAAGAATCTGTCCGCGATCA11.098901098901099No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT11.098901098901099No Hit
TGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA11.098901098901099Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCAGTACTCTGCGTTGATA11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
CCATTACTCTGCGTTGATACCACTGCTTCCCATTACTCTGCGTTGATACC11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
CGAGCCTGATGGGCGTCTCGCCGCCGCTTCCGATCAGCGCCTGGCCGTCG11.098901098901099No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
GGATGCAGGCAGATTTTAAGTGCTGTGGGGCTACTAACTACACAGATTGG11.098901098901099No Hit
ATGCCAAACGGTAGAGAGTTGTGGAATTTTTTAGCTCCACATAGACCGAT11.098901098901099No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCAATTACTCAGCCTTTTTA11.098901098901099Clontech SMART CDS Primer II A (96% over 26bp)
GCAAAATAGCGTGCATTGTTTTGAAATCCGCGTTGATTTTGCGGGCAGGA11.098901098901099No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA11.098901098901099No Hit
ACCTGACGCTGTACATCGACCGCAACGAGCTGCGCGTGATCATCGGCCCC11.098901098901099No Hit
GGACGCAGCTACGCGGTGCTGTTGGGCATCATCTTTGGGGGCCTCGCGGT11.098901098901099No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
CATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGA11.098901098901099No Hit
GTACATGGGAAGCAGTGGTTCAACGCAGAGTACATGGGAAGCAGTGGTAT11.098901098901099No Hit
AGCCTCCTCCATATGGCAGGAATCAAGCTTTCACAGGGGAAACGCAGGAT11.098901098901099No Hit
ATATAAGTCCGTGAGAGTAGTTAATATATCCTGCGTATTGCTTAGGGTTA11.098901098901099No Hit
TCCTGGATACGGGCACGTCCCTCATCACAGGACCCACTGAGGAGATCCGG11.098901098901099No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA11.098901098901099No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGATGATACCAATC11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
GGCCTGCAGAGAGCATTCCATCTGGAAGTTGTGGACTTTTCTAGTGAGAG11.098901098901099No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCCATGTACTCTGCGTTGA11.098901098901099Clontech Universal Primer Mix Long (96% over 27bp)
GGTCGGCAAAACCATCGTCTTCGCCCAGCGTCAGGACCACGCCGAGCACC11.098901098901099No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
CACCCGGGCCAGCTGGGTCGCGTTGTTGACGGGGCCGAACAGCGCGTCCT11.098901098901099No Hit
GTCCAGGATGTGCAGAAGTTTGCCCGGCTCTCGGCCCAGCTGACACACGC11.098901098901099No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA11.098901098901099Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTGTGGTATCAACGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA11.098901098901099No Hit
CTCCGGAGGGGACCCGGCCTTCCTGGGCATGGCCGTGAGCACCCTGTGTG11.098901098901099No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA11.098901098901099Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGTAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11.098901098901099Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGACTC11.098901098901099Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAATCAGTGGTA11.098901098901099Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCATGTCCCGCATA11.098901098901099No Hit
GAGACAGACAAGGCCGACACAGGGCTGGGGGCCCGTGGTCCACCAGTGGA11.098901098901099No Hit
CATTTTTGACATTGATGCCAATGGTATACTCCATGTCTCTGCTGTGGACA11.098901098901099No Hit
AACTCTCCCAGCACTGCACCCCAGGCAGCCACTCTTAGCCTTGGCCTTCG11.098901098901099No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)