FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003634970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003634970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA55.4945054945054945Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA44.395604395604396Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC44.395604395604396Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT44.395604395604396Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG33.296703296703297Clontech Universal Primer Mix Long (96% over 26bp)
GGAGGAAGGTGGCAGAGGGAACTCCACACCATTGATGATGAAGGTCAAGC22.197802197802198No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA22.197802197802198No Hit
GGCATGAACTGCAGGACGCTGCCACGGCCACCCTGCTCCAGGCAGTCCAC22.197802197802198No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA22.197802197802198Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG22.197802197802198Clontech Universal Primer Mix Long (96% over 26bp)
GATAGGAACTACAAGGTTGGAGAGGGTCGTTTGCCATGGCCGAGCACCAT22.197802197802198No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT22.197802197802198Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT22.197802197802198Clontech Universal Primer Mix Long (96% over 25bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC22.197802197802198Clontech Universal Primer Mix Long (96% over 26bp)
GCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTG22.197802197802198No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA11.098901098901099Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
AGGGTGGGGTTACCAGTTCGTGGCGAGGGCTTCCAGAGTGTTGATCCGAT11.098901098901099No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT11.098901098901099Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTGGTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCATGGGTCT11.098901098901099No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG11.098901098901099No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTGCTTCCCAAGTACTCTGC11.098901098901099Clontech SMART CDS Primer II A (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC11.098901098901099Clontech SMART CDS Primer II A (96% over 26bp)
ATTTCTCCGTCTTTTACTTCTAAAGCTATTCCTCCTTTAGCAGCAAATGC11.098901098901099No Hit
GTACTTTTTTTTTTTTTTTTTTGAGTTGATACCACTGCCCCATGTACTCG11.098901098901099No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
AATCAACATGGTTCAACCGCCTGACGAGTGCTGACAGCTATGAAGAAGAC11.098901098901099No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT11.098901098901099No Hit
GAGCGCTGCTAGCACGGGGCTTGGGTGTTGGAACACTGGGCCTCCTGCCT11.098901098901099No Hit
AATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA11.098901098901099Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC11.098901098901099Clontech SMART CDS Primer II A (96% over 26bp)
GCTGAGCCCTGCTCTCCTGGAGCTCACAGCCAAGTCCACTTCTTCCATCT11.098901098901099No Hit
GTACTTTTTTTTTTTTTTTTGCGTTGATACCACTGCCCCATGTACTCTGC11.098901098901099No Hit
GGCTCCGGTCGTGGTCTTCGACGACGCCGACATCGAGGCCGCGGTCGAGG11.098901098901099No Hit
AGATACAAGAAACCTGAGTGCCAGAGTGACTGGCAGTGTCCAGGGAAGAA11.098901098901099No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTAAATGGCAAGCAGAGGTA11.098901098901099Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG11.098901098901099No Hit
CCCTGGAGCAGGACCTGGCCTTCTGGTACGGGCCCCGCTGGCAGGAGGTC11.098901098901099No Hit
GCCACATACGTCCCCAAGAAGACGTCACCGAGGATCCAGAAGGGCCCTGC11.098901098901099No Hit
GCCTGGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGC11.098901098901099No Hit
CCTGGAACGACGACCTCGACGTGCTCACCCCGGCGATGGGGGCGCTGGCC11.098901098901099No Hit
TCGTTATCCGGTTTTTTGAATTCCTTGATCAGGGTGTCCACGTGCGGGAT11.098901098901099No Hit
TCGTTAATCTTGCCTTGAAGTTTCTCATCTTCAACAGTTGCTTTCATGTT11.098901098901099No Hit
GTACATGGGAAGCAGTTGTATCAACGCAGAGTCATGGAAGCAGTGGTATC11.098901098901099Clontech Universal Primer Mix Long (95% over 23bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
GTCCTATGTAAAAACTGACAATCATCAACAGGTCATTGAATTACGTTTTG11.098901098901099No Hit
CCCCAGATGAGGAGGCTGAGGAGACCAGACCCCTACATCACCTCGTAGCC11.098901098901099No Hit
GAGTAATGGGAAGCAGTGGTATCAACGCAGAGTAATGGGAAGCAGTGGTA11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT11.098901098901099Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCTGTGTCCCCACCAAGTGAGATGGAATAAATGAGAGTCCTTTGGAGGC11.098901098901099No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
CGTTGATACCACTGCTTGCCCATGTACTCTGCGTGTGTATCTGATACACA11.098901098901099Clontech Universal Primer Mix Long (95% over 21bp)
TTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGA11.098901098901099No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC11.098901098901099Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGAT11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
CGCCGAACACCGAACGCCCTTTGGGAAACGACACCTCCAGGTTCTGGAAG11.098901098901099No Hit
TCCCTGAAATCTGCCTCTCTCTGTCATTATGCTTGGCCGGTACTGCTCAT11.098901098901099No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTTACCCATTCTTA11.098901098901099No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.098901098901099No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
GTCTGTTAAGATAAAGTTTACAGCAGGAAAGTATTTAGAAAGCGAATCAG11.098901098901099No Hit
TGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.098901098901099Clontech Universal Primer Mix Long (95% over 21bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)