FastQCFastQC Report
Tue 10 May 2022
EGAF00003635010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003635010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334168
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACAT9110.2726173661152474No Hit
TCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGTTTTCCAC8710.26064733906298626No Hit
CAATTAATCTTTCCATTTTCGTCTGCGTTTAGTAAATGCGTTAACTAGGC8430.2522683201264035No Hit
CTTCTATCTTCTCCAAATTGTTTCATCCTACCACTCCCAATTAATCTTTC7080.21186947882502216No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT6750.20199420650690672No Hit
GTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTT6410.19181968351248474No Hit
AATTAATACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACT5680.16997438414210816No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA5410.1618946158818319No Hit
ATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGTTTTCCACTTCAA5390.16129611452921885No Hit
GTTTAAGTCACCTTCATTTTAATCTAAAAGCATTGCCCTTCTATTGGTAT5290.15830360776615354No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG4590.13735606042469656No Hit
TTCCCGTACTTCTGTCTTCCAGTTTTCCACTTCAAACTTCTATCTTCTCC4580.13705680974839002No Hit
CCCGTACTTCTGTCTTCCAGTTTTCCACTTCAAACTTCTATCTTCTCCAA4570.1367575590720835No Hit
CTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAG4420.13226879892748558No Hit
GACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACA4410.13196954825117907No Hit
GTACATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG4280.12807928945919417No Hit
TCCTACCACTCCCAATTAATCTTTCCATTTTCGTCTGCGTTTAGTAAATG4250.12718153743027458No Hit
GTATTAATTCGGGGCTCTGTAGTCCTTTCTCTCAATTTTCTTTTAAATAC4040.12089727322783748No Hit
GCTTTAAATGACGCAATTCTCCCTGCGTCATGGATTTCAAGGTCTTTTAA3900.11670776375954608No Hit
GATAAAAGGTTTCTAAAACATGACGGAGGTTGAGATGAAGCTTCTTCATG3780.11311675564386775No Hit
GTTTTTATCTTCTAATTTCCCTATCTTCTCTATTCTTTTCTTCGCCTTCC3710.11102200090972204No Hit
GCTTAAGATTTTAAGAGAAAATATGAAGACTTAGAAGAGTAGCATGAGGA3670.10982499820449594No Hit
GCGTTAACTAGGCTTTAAATGACGCAATTCTCCCTGCGTCATGGATTTCA3650.10922649685188289No Hit
TCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATTGAGAGAAAGGACTA3620.1083287448229633No Hit
GAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACATGACGGAGG3600.10773024347035023No Hit
GAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACATGACGG3480.1041392353546719No Hit
GCGTTTAGTAAATGCGTTAACTAGGCTTTAAATGACGCAATTCTCCCTGC3440.10294223264944578No Hit
GGTTGAGATGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAAT3380.10114672859160663No Hit
CTTTTAATCACCTTCGGTTTAATCTCTTTTTAAAAGATCGCCTTCAAATT3350.10024897656268704No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)