FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003635213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003635213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA297.107843137254902Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG204.901960784313726Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT143.431372549019608Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT81.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG71.715686274509804Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG61.4705882352941175Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC61.4705882352941175Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG51.2254901960784315Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGAAATAGAGAGAGACAAGGACGGCGAGTCCAAAGAGGATTTTCTCTAC51.2254901960784315No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51.2254901960784315No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC51.2254901960784315Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT51.2254901960784315Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC51.2254901960784315Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG40.9803921568627451Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC40.9803921568627451Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA30.7352941176470588Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA30.7352941176470588Clontech Universal Primer Mix Long (96% over 26bp)
GACTCCAGCACATGGCCGCATTAGCCAACTGAGGACGTATGGAGGTCTAT30.7352941176470588No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG30.7352941176470588Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTTACTCATCTGCTCCAAGGCTTTCAAAGGCTCTCTTCTGCCTGTGGAAG30.7352941176470588No Hit
ATCCTGTACTGTGTGGTGACGTCCCCTATGTTGCTGGTGGCTCTGGCTGT30.7352941176470588No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC30.7352941176470588Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACAGGGAAAGCAGTGGTA30.7352941176470588Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGCAGTTGCATCTTTAGGCTTTTTCTGAA30.7352941176470588No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA30.7352941176470588Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT30.7352941176470588Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG30.7352941176470588Clontech Universal Primer Mix Long (96% over 26bp)
GATACATGCCAGTGAGGGAAGATGCAGCTGGAGAAGTCAACAGCAATCAT20.49019607843137253No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG20.49019607843137253Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT20.49019607843137253Clontech Universal Primer Mix Long (96% over 25bp)
ACACGGTGAAACCCCGTCTCTACTAAAATATAAAAAATTAACCGGGTGTG20.49019607843137253No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT20.49019607843137253Clontech Universal Primer Mix Long (96% over 26bp)
AGTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAGTATTAGTTA20.49019607843137253No Hit
GTCGTACAGGGTGAGTACAGATGCACGGGAGGCCATACGGTTTAGGCAAA20.49019607843137253No Hit
GGATATCAGGAGGTGCGTACACCGCTGTTGCTCAACCAGAACCTATGGAA20.49019607843137253No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT20.49019607843137253Clontech Universal Primer Mix Long (100% over 23bp)
GTGGAAGTGCAGATGACACGCAATAGGCTGTGTGCTGGTTAATTTTGAGG20.49019607843137253No Hit
CTTGGTGGCCGGGCATGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGA20.49019607843137253No Hit
GATAATGGAGTTACAAAACATTCTAAATTCTCATTTCGTTAGGTTTTGTC20.49019607843137253No Hit
TGCTACTATTATGAACAACATAGTCATTGTAGCTTAGCAAAGGACATGAA20.49019607843137253No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG20.49019607843137253Clontech Universal Primer Mix Long (96% over 26bp)
CCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTCCCGCGG20.49019607843137253No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA20.49019607843137253Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCT20.49019607843137253Clontech Universal Primer Mix Long (96% over 26bp)
CCACCTTTATTCCACAAGATCCCTCTAAATGGCTTAATCATAGGCACCCT20.49019607843137253No Hit
GGACTCTGTAGTCTCTTTGGGGTCTGGGGAAGGATGTGGTGGCAGAGCCA20.49019607843137253No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG20.49019607843137253Clontech Universal Primer Mix Long (96% over 26bp)
CTACAGGCACCCGCCATCACACCCAGCTAATTTTTTTGTATTTTTAGTAG20.49019607843137253No Hit
GAGTAACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG20.49019607843137253Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20.49019607843137253Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC20.49019607843137253Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA20.49019607843137253Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGAGGGCTGCCAATGGGACAAAACAAAATTCCTTCTATCATGTACCA20.49019607843137253No Hit
GATGAAACCCAGACACATAGCAATTCAGGAAATTTGACTTTCCATTCTCT20.49019607843137253No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT20.49019607843137253Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGCTCGGGAGTTCAAGACCAGCCTGGGCAACATAGTGAAACCGTCTCCA20.49019607843137253No Hit
GTCTTAAGACCTCAGGAGACCACTTCTTTAGCAAGCAATTTTGTTTTTTG20.49019607843137253No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG20.49019607843137253No Hit
CCGCAAGTACTATTCCACCACCCACGGTGCTCTGCTGTCCGGCCAGCGTG20.49019607843137253No Hit
GATACCACTGCTGTACTTTTTTTTTTTTTTTTTTTGAGACAGAGTCTTGC20.49019607843137253No Hit
TTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT20.49019607843137253Clontech Universal Primer Mix Long (96% over 26bp)
ATACTGCACCTTTCCATCAAGTGTCACCCGACGTGCCAAAACTCGGTATT20.49019607843137253No Hit
CCCATGTACTCAGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGAT20.49019607843137253Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGTTAGAGATGGAACAGAGCGTGAAAGTACGGACCATGAGGTGACTGTT10.24509803921568626No Hit
CTCTTACTACAATAGCTGTGTGACATTGGACACATGCCTTTACTTCCCCA10.24509803921568626No Hit
CCACACAGTTGTTGCTGCCTATTGTGGTGCCGCCTCAGGTTAGGGGCTCC10.24509803921568626No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCTGTCTCTTATACACATCTC10.24509803921568626Clontech SMARTer II A Oligonucleotide (100% over 22bp)
ATTAAAAAGAATTCTCATCTTGACTCAGGATTGTTGAACACTATTGATAT10.24509803921568626No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGG10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
CCAATAGCGTATATTAAAGTTGCTGGAGTTAAAAATCGCGTAGTTGGGTG10.24509803921568626No Hit
GTCACGCACCGCACGTTCGTGGGGAACCTGGCGCTAAACCATTCGTAGAC10.24509803921568626No Hit
GGGGTGACAGCTTGAGGGGTGACCGAGCCCTCCCGGCCGCTCCCGGATGC10.24509803921568626No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGTAAGCAGTGGTATC10.24509803921568626Clontech Universal Primer Mix Long (96% over 25bp)
CGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAAT10.24509803921568626No Hit
ACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10.24509803921568626Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTCATTGCATCATTAATTTCAAATGGCAAGGGAAAGAGAATGTATTAAA10.24509803921568626No Hit
CTATAGAGGTATTTTGTTCTTAAAGCTATTTCAAAGGCAAACCAAAACAA10.24509803921568626No Hit
TCCCATTACTCTGCGTTGATACCACTGCTTCCCATTACTCTGCGTTGATA10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
GCCCACCTTGGCCTCCCAAAGTGTTGGGATTACAGGTGTGAGCCACTGTG10.24509803921568626No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTACCATCTAGACAGC10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG10.24509803921568626Clontech SMART CDS Primer II A (96% over 26bp)
ATACCACTGCTTCCCTGTACTCTGCGTTGATACCACTGCTTCCCATGTAC10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGAGTTGATACCACTGCTTCCCATGTAC10.24509803921568626Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTTTGCTTTGGCTGTTAACAACAAGATGGCTCTTAGGCATCCAAATGGAA10.24509803921568626No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCACTGT10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
GTACAGACAAAGTGGGAGGTTTTATTTCTTGGTCTCTTCCTCCTTGGATA10.24509803921568626No Hit
CGTTTGCACATACATTTCTGTGCATGAAAATCTGTGCATGCCGAATTTGA10.24509803921568626No Hit
AGTCCCAGCTACTCAGAAGGCTGAGGTGGGAGGATCACCTGAGTCTGGGA10.24509803921568626No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTGTCTCTTATA10.24509803921568626No Hit
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
TGGCTACCTTGAGTGCTCAGCCAAGACCAAGGAGGGAGTGCGGGAGGTGT10.24509803921568626No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAATTACACTGCGTTGAAACCTGTCAC10.24509803921568626No Hit
GTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTT10.24509803921568626No Hit
CCTATTATTATACATATGTATTTTTCTTTATTTTTCCCTCTCTGTTTTTT10.24509803921568626No Hit
CTTAAACACTGTGCTGAAGACTACTGTGACTCTGTCCTTTTTAGACTCAA10.24509803921568626No Hit
TCTTTACGCCAGCACTGGCGGGGAGGAGTGCCCAGGAATCTGACAAGGGC10.24509803921568626No Hit
GTATACCAGCAGACACAAAATTACTTCCAAAGCTCCCATTGGTGTAATTT10.24509803921568626No Hit
GAGTTGCCACTGTATGCACAGGGGATGAGCAGCTGCCGGTACTCCAGGGG10.24509803921568626No Hit
TACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
GTCACGTGATCATTCTCCGATTTCGGCGCCCGAGGCCGTCGCCGTGGCCT10.24509803921568626No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGCTGTCTCTTAT10.24509803921568626Clontech SMART CDS Primer II A (96% over 26bp)
CTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGA10.24509803921568626Clontech SMART CDS Primer II A (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTGTCTCTTATACA10.24509803921568626Clontech SMART CDS Primer II A (96% over 25bp)
GCTAACGGGAATGCTAATGAGGAAAATGGGGAGCAGGAGGCTGACAATGA10.24509803921568626No Hit
GTACAGAGAAGCACCTATTGACAAAAAGGGGAATTTCAATTACATCGAGT10.24509803921568626No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTT10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACAGAAAAAAAAAA10.24509803921568626No Hit
GCCCACTACCACGCCTGGATAATTTTTTGTATTTTTTAGTAGCTGTCTCT10.24509803921568626No Hit
GTACATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAGTAA10.24509803921568626No Hit
AGTATATTGTGTACTGGTGGACATTGGGCTTCTAGCATACCCGTTAACCC10.24509803921568626No Hit
GCTGATAGTTTCTTTTGCTGTGTAGAAGCTCTTCAGTTTAATTAGATCCC10.24509803921568626No Hit
GCTACCATGCCTGGTCCAGTAGTATCTTTTCTATCTCAAAAATAATATAG10.24509803921568626No Hit
GTACATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAGTAC10.24509803921568626No Hit
GTATCAACGCAGAGTACTGGGAAGCAGTGGTATCAACGCAGAGTACTGGG10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
CAGCCGAATTCTACCAGAGGTACAAGAAGGAGCTGGTACCATATTTTCTG10.24509803921568626No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCACTCCT10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTGCGTTGATACCACTGCTCCCATT10.24509803921568626No Hit
GGCCTAAGCCTTCCCAGGATCTTCAGGACACTTGACAGACTTGTGTTTTG10.24509803921568626No Hit
GTACTATATGCAGTGTGATTTAAGCCAAGTTATTTCACGGTAAGGAGGAA10.24509803921568626No Hit
GTTTTAAGAGCGTTTGAATGGAAAAACTTCGACCAAGTTTGGACACAACC10.24509803921568626No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAATG10.24509803921568626No Hit
GTACATGGGAGAAGAAGAGACGATGAAGACGACGAAGACGAAGAAGATGA10.24509803921568626No Hit
GTACATGACTGTATCTCTATCCTATTGGTTCTGTCGCTCCGGAGGATGCT10.24509803921568626No Hit
ACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGATACCACTG10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGC10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACCTG10.24509803921568626Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATCTATAACCAAGAGGAGTCCGTGCGCTTCGACAGCGACGTGGGGGAGT10.24509803921568626No Hit
ATGTCGTCTGGTCCCTGTTCAACACCCTCTTCATGAACCCCTGCTGCCTG10.24509803921568626No Hit
TCAAAGGAGAGAATAACTCAGGAAATTAATTTGCTCTTAATCTCTGGATT10.24509803921568626No Hit
TGAAGAGCCTGCCGGACTGGACGGTGCAAAATGGGAAGAACCTGACCCTG10.24509803921568626No Hit
ATCATGAGGTCAGGAGCTGACCAGCCTGGCCAACATAGTGAAACCCCATC10.24509803921568626No Hit
GGGATGCACAGAGTTTGAGATGTGCTGTGTATCGCCATGTTGCCCTTACA10.24509803921568626No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACCTGTCTCTTATACACA10.24509803921568626Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTCCCGCGG10.24509803921568626No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACTCAGAGTACAG10.24509803921568626Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGCCTAAGCCTTCCCAGGATCTTCAGGACACTTGACAGACTTGTTGTTTT10.24509803921568626No Hit
GTCTAAAAATGAGATCCGAATTCTCATGATCGTTCTTACGAGCTGTCTCT10.24509803921568626No Hit
GGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAATAAA10.24509803921568626Clontech Universal Primer Mix Long (95% over 21bp)
GAATATCTTCATATAAAATCAAGACAGAAGCATTCTCGGAAACATCTCTG10.24509803921568626No Hit
GCCCTGGGCTGGAACCGGCTGGACTACATCGTCTCGAGCAAGTTCTTCTG10.24509803921568626No Hit
GTACATGGGTAAGCAGTGGTATCAACACAGAGTACATGGGAAGCAGTGGT10.24509803921568626Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCT10.24509803921568626No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTAACAACAT10.24509803921568626No Hit
CCGCGGTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGC10.24509803921568626No Hit
GTACATGGGAAGAAGTGGTATCAACGCAGAGTACATGGGAAGCGCAGTGG10.24509803921568626Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTT10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
AGCTGAAACAGATGAGGGTCTGTGCCACAAAGTCAGAATAATAAAGAGCA10.24509803921568626No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTGTCTCTTAT10.24509803921568626Clontech SMART CDS Primer II A (96% over 25bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTT10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
TCCCTAAATCTCCTTAAGCTGATAAGCAACTTCAGCAAAGTCTCAGGATA10.24509803921568626No Hit
CTACTAGACATTGCCATGTCAAATATCTTATGTTATCCTCACAAGAACCA10.24509803921568626No Hit
GGCGAATCCAAATTACAGAGAAAGAGAATCCAGCTGAAGGGCCAATGAAG10.24509803921568626No Hit
GAGAAGTCCTGTTTCAGAAGAGCAGCCTGCCTCCTTCTCCTCCATCTTCT10.24509803921568626No Hit
ATCTCATTGTGGATTTAATTTGCATTTCCCTGTTGTCAGTGAAGCTAGGC10.24509803921568626No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.24509803921568626Clontech Universal Primer Mix Long (95% over 21bp)
GTACATGGGAAGCTGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.24509803921568626No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.24509803921568626No Hit
GTGGTGAGATCTCGGCTCACTGCGAGCTCTGCCTCCCTGGTTCAAGCAAT10.24509803921568626No Hit
CTTTGTAGCCTTCATCAGGGTTTGCTGAAGATGGCGGTATATAGGCTGAG10.24509803921568626No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAATTACTC10.24509803921568626No Hit
GGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAGTAA10.24509803921568626Clontech Universal Primer Mix Long (95% over 21bp)
GTCCCAGGCAAGCACCTTCCTCAGATTTCAGGTCTAATAGTTCATCTATG10.24509803921568626No Hit
GCTTTAGGATCCTTTTCTCTCTCTGCAAAAAGTCCCTCGATAGCTGGAAA10.24509803921568626No Hit
CGTTGATACCCACTGCTTCCCATGTACCTGTCTCTTATACACATCTCCGA10.24509803921568626No Hit
TCGCGCGGGGGCACCCTGGGCACCCCTCAGACTGGCTCGGAGAACGACGC10.24509803921568626No Hit
AGTTAAACCACAGCAACCCTGCAATGAGAGGGACAAGCCTCACTTTTCCA10.24509803921568626No Hit
TAAGGAAGGAAAGAAGGAAGAGGAAAGGAAGGAAGAAAGGAAGGAAAGAG10.24509803921568626No Hit
GGGTGCCGGTGAGGATGACAGTGCCGGGGGCGAGGGTTTTGCTCGCGCTG10.24509803921568626No Hit
GGAAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.24509803921568626Clontech Universal Primer Mix Long (95% over 22bp)
ACATAAAGTTAAATTGCCTTTGTTGCTCTCCATCAAAGCTGCTGTTTTGT10.24509803921568626No Hit
TTGAACAGGAGTACCGTTTGTATTCTGACGAGACCAAGCGGTTATCAAGG10.24509803921568626No Hit
TTGCAGAGTGCCCATGGAAGACGGGGATAAGCGCTGTAAGCTTCTGCTGG10.24509803921568626No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATTTAAAAACAAACGAACA10.24509803921568626No Hit
ACTCTGCGTTGATACCACTGCTTCCCAAGTACTATGCGTTGATACCCTGT10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGC10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCGCAGTGG10.24509803921568626Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCGAGTAGTG10.24509803921568626Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACTCTGCGTTGATACCACTGCTTACCCATGTACTCTGCGTTGATACCACT10.24509803921568626Clontech Universal Primer Mix Long (96% over 27bp)
AGTCTCCACAGAAAGCCTGAGCAGAATGAAGGCTTTGGTGGCACAGGGAA10.24509803921568626No Hit
ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA10.24509803921568626Clontech Universal Primer Mix Long (95% over 21bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTTATACA10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA10.24509803921568626No Hit
ATACCTGTCCCAGTGATAAGGAGGTTGAAATAGCATATAGTGATGTAGCC10.24509803921568626No Hit
GTGGTATCAACGCAGAGTACTTTTTTTCATACGCTTATGGCCACACATAT10.24509803921568626Clontech SMART CDS Primer II A (100% over 21bp)
CGTAAGAAGATGGAGGTGGGCAAAGTGGATTGTAAAATGATTTTGAACAA10.24509803921568626No Hit
GGCTAGAGTGCAGTGGCGTGACCTCGGCTCACTGCAACCTCCACCTCCCG10.24509803921568626No Hit
TATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGG10.24509803921568626Clontech SMART CDS Primer II A (100% over 26bp)
CCATTGATCTTGCACAGTTCTTCATCAATCACATCCCACATGGCCGTGCC10.24509803921568626No Hit
CTGTTCGGCCTGCTCGACGATCTGCAACTGCCCGAATCGCATGGGCCGCA10.24509803921568626No Hit
ATTTTATAGATAAGGAATGTGAATTTTACAACAGTTAAGTACCAAGGCCA10.24509803921568626No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTCTCTTATA10.24509803921568626Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAGTACTC10.24509803921568626No Hit
CCAATAGCGTATATTAAAGTTGCTGCCGTTAAAAATCGCGTAGTTGGGTG10.24509803921568626No Hit
TCTTTAGAGATTTCCTTTGAATGTTTGCTTCTGTTCTGCAGCATTTTCAG10.24509803921568626No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGTTA10.24509803921568626Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
CCTCAGCTTCGTGCTGGGGCGCCCTGCCGTACGCAACGGCGTCTACGAGA10.24509803921568626No Hit
GTGGAGTGCAAAGGTTGCAGGCTATGGGCGGCTACTAGTAACCCCGTTTT10.24509803921568626No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA10.24509803921568626Clontech SMART CDS Primer II A (96% over 26bp)
GCCCTGGGCTGGAACCGGCTGGACTACATCGTCTCGAGCACGTTCTTCTG10.24509803921568626No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
TACAAGGGGTGTCCCTGCTATTTTCCTAGACCTGTTTCCTTAGTTTTCTC10.24509803921568626No Hit
AGTGGTATCAACGCCGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.24509803921568626Clontech SMART CDS Primer II A (95% over 22bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGAATCAA10.24509803921568626Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
GATGCCGACCTCGGGGTGGCCCGGCGTCTTGCTGTGCAGCTGGCGGAAGG10.24509803921568626No Hit
GTCCTAAAACTGGTCTGTATACCAGCAGACACAAAATTACTTCCAAAGCT10.24509803921568626No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGCTGTCTCTTATACA10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG10.24509803921568626No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG10.24509803921568626No Hit
GCTCAGCGACGAGCGTGCCGCGGCCTACATTGCCGAGTTGAAGGCCGACA10.24509803921568626No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA10.24509803921568626Clontech SMART CDS Primer II A (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTCTCTTATA10.24509803921568626Clontech SMART CDS Primer II A (96% over 26bp)
CAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA10.24509803921568626No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
ATGTAAAGGGTGGGGTTATGCTGTGATATTAATTTTCCTCAATAATGTTT10.24509803921568626No Hit
ACGCAGAGTACATGGGACGCAGTGGTATCAACGCAGAGTACATGGGAAGC10.24509803921568626Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTGGGGGTTGGA10.24509803921568626No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTGTTAACAC10.24509803921568626No Hit
AACCAGCAGGGAGTCGGGATACCCAGGGCATCGCTTTCCCATCTAGTGTC10.24509803921568626No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTACATGTACACTGCGTTGATA10.24509803921568626Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGGGAGTACATGGGAAGCAGTGGTATCAACG10.24509803921568626No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGTGT10.24509803921568626No Hit
GAGGAAATATTGGGAAATTGGGTGAACGTCAGGTGGATAAGAGAGATGCA10.24509803921568626No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTTTACCACTGCGT10.24509803921568626No Hit
ACATAGGGAAACCCTGTCTCTACAAAAAATACAAAAAACTAGCTGGGAAG10.24509803921568626No Hit
TATCAACGCAGAGTACTGGGAAGCAGTGGTATCAACGCAGAGTACTGGGA10.24509803921568626Clontech SMART CDS Primer II A (100% over 26bp)
GGCATGAGGGGCTGTGCACCCTGACAGAGCCACCCAGGGCCAGTGGCCTG10.24509803921568626No Hit
GTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCC10.24509803921568626No Hit
TCCCAGCTTCCTAATCCCTCAGCTGGGGTAACTCAGACGGGTCTACATTG10.24509803921568626No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAGCCGAGGAAATTGGGAGC10.24509803921568626No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)