FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003635214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003635214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT307.352941176470589Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC143.431372549019608Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA133.1862745098039214Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG102.450980392156863Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG81.9607843137254901Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG81.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC71.715686274509804Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC51.2254901960784315Clontech Universal Primer Mix Long (96% over 26bp)
CTTTGAAATCCGATTCTTATGACACCGCGGAGGTCACCTGCCTCCGCTTT51.2254901960784315No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT51.2254901960784315Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC51.2254901960784315Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT40.9803921568627451Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA40.9803921568627451Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA40.9803921568627451Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG40.9803921568627451Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC40.9803921568627451Clontech Universal Primer Mix Long (96% over 26bp)
GGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACC30.7352941176470588No Hit
GCGCTGTGGGCCCGAGCAGCAGAGCCGTGCAGGACAGGCATGGGCAGGGG30.7352941176470588No Hit
CACTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT30.7352941176470588Clontech Universal Primer Mix Long (96% over 26bp)
GTATGTATGAGGTACCTGGGCAAAGAAGAATTTTCAAATAATCTAAATAA30.7352941176470588No Hit
TTCAAAGCCTTCCTTAATATTTGGAGAGCATTAGTGGCACACCAGCAGTT30.7352941176470588No Hit
CCCCACCGCGACTGGCTTGGGTTCTTTGTGCTTGATCCACCGTGAGTTTG30.7352941176470588No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG30.7352941176470588Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGGTACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC30.7352941176470588Clontech SMART CDS Primer II A (96% over 26bp)
CTCACGGGTTGAAGAGAGTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGAC20.49019607843137253No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG20.49019607843137253Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA20.49019607843137253Clontech Universal Primer Mix Long (96% over 26bp)
GGAGGGCGATGAGGACTAGGATGATGGCGGGCAGGATAGTTCAGACGGTT20.49019607843137253No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG20.49019607843137253Clontech Universal Primer Mix Long (96% over 26bp)
CCGCATCGTTGCGGGCCTTGTCCTGCAGCTGCTCGCGCATCCAGGCCATG20.49019607843137253No Hit
TAATGATTTGGCCCAGAGCAGTGGCTCACGCCTGTAATCCCAGCACTTTG20.49019607843137253No Hit
CTCTCATAGTTTTTCCAGCCCTCCTAGTCACAATCCCCAGAGGCAGCTAC20.49019607843137253No Hit
GAAGGCTAACACTTCAGAGGGTCAGCTGAGAAACTCTATGGTCACATTTA20.49019607843137253No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT20.49019607843137253Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.49019607843137253Clontech Universal Primer Mix Long (95% over 22bp)
AGCCTGACCAACATGGTGAAACACCATCTCTACTAAAAATGCAAAAATTA20.49019607843137253No Hit
CAAGAAACTGAATTCCCTCACTCAAACCATAAACAAAATTCAGTGCCAGG20.49019607843137253No Hit
CACAGAGACAGAGAGGGAGACAGAAAGAGAGATAGAAGCATACCCAGTGA20.49019607843137253No Hit
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT20.49019607843137253Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA20.49019607843137253Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT20.49019607843137253No Hit
CTCTTCCTTGGGGCCGGGCTGTTCATCTACTTCAGGAATCAGAAAGGACA20.49019607843137253No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.49019607843137253No Hit
GTGGCTCACGCCTGTAATCCCAGCACTTCAGGAGGCTGAGGTGGGCGGAT20.49019607843137253No Hit
GTCTGGGTGAATGACCCTTCATGACAAATGCCATATATGGTACATGATAG20.49019607843137253No Hit
CCTAGGGGGAACCCTAGCTCCGCCTGTCACAGCTGTGTGGCCTCAGGCAA20.49019607843137253No Hit
CTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCA20.49019607843137253Clontech SMART CDS Primer II A (100% over 26bp)
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20.49019607843137253Clontech Universal Primer Mix Long (96% over 26bp)
GATCCAGCCATGTGTGATTAATTTCTAAATGTGGAAATCAAAGCCAGCTT20.49019607843137253No Hit
GGCTGGAGTGGTGGTGAAATCACGGCTCACTGCAGCCTCAACCTCCTGGT20.49019607843137253No Hit
ATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAA20.49019607843137253No Hit
TGACCAAACTCTGCCAGACGTAGAGGAAGCTCGCGATAAGAACGAATCTC20.49019607843137253No Hit
CATCTGAAATGGTGGATTTTCGCTTTTGCAGAGAAATGCCCCCGAAGAGA20.49019607843137253No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACCT20.49019607843137253Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT20.49019607843137253Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTC20.49019607843137253No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT20.49019607843137253Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCTGAAGGCAGTCAGGGCGGCTAGCTCTACAGAAGATAAAAATCCTTTC20.49019607843137253No Hit
TTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGG20.49019607843137253No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG20.49019607843137253Clontech Universal Primer Mix Long (96% over 26bp)
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.49019607843137253No Hit
GTACATGGGAAGATGATGAAGGCAAAGAAGATTATCAGTTTGATGAACTC20.49019607843137253No Hit
ACTGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT10.24509803921568626Clontech SMART CDS Primer II A (100% over 26bp)
ACGCAGAGTACTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
CTTAATCATTGATTATAGATTAAAACTGAAACAACATGAAGGGATCAGAC10.24509803921568626No Hit
CTGTTAATACTGGCCTCACAGGCCATAATCTCTTATGTGTCTTTTTGTAT10.24509803921568626No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGAT10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
AAACCACTCACAGAGCAGACCAGGAGGTTGTGGTGCTGCAGGGGCTGGGT10.24509803921568626No Hit
GTATCTACTATTTGTCAAGCACTCAAAAAGCTCACATGGTTTTCAACAGC10.24509803921568626No Hit
TAAAAACCCTAGAAGAAAACCTAGGCAATACCATTCAGACATAGGCGTGG10.24509803921568626No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACGCTGCGTTGATAACAC10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGA10.24509803921568626No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTT10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTAT10.24509803921568626Clontech SMARTer II A Oligonucleotide (100% over 22bp)
CTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATT10.24509803921568626No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTTAAA10.24509803921568626No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG10.24509803921568626Clontech SMART CDS Primer II A (96% over 26bp)
GTACAATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACTCAGAGTACATGGGAAGCAGTGGTATCAA10.24509803921568626Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGGGTTTTAAAC10.24509803921568626No Hit
GAGTACATGGGGAACCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA10.24509803921568626No Hit
AGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAA10.24509803921568626No Hit
CCTTTTTCACCATAGGCCTCAAAGCGCTCGAAATGTCCGCTTCCAGGTAG10.24509803921568626No Hit
GTGCGTGGCATTAATATTAATATTGTGACTCGCTCAGGAATTGAGTGATG10.24509803921568626No Hit
CTCCACATGTATCACAGCCTGTACCTGAAGGTGAAGGGGAATGTGTTCAA10.24509803921568626No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGT10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTGGGTGGATATTCAAC10.24509803921568626No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCAGTTAACTGCCTTTAAA10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTAACACAATA10.24509803921568626No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACCTGGGAAGCAGTGGTATC10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
AGGTAGTGGAGCGCCGCTGCTTACCTGGGTGCAGGAGACAGCCGGAGTCG10.24509803921568626No Hit
TGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAATTACTAGGG10.24509803921568626No Hit
GTACATGGGAAAGCAGTGGTATCAACGCAGAGTACATGGGAAAGCAGTGG10.24509803921568626Clontech Universal Primer Mix Long (96% over 27bp)
GTACATGGGAAGCAGTGGGTATCAACGCTGTCTCTTATACACATCTGACG10.24509803921568626No Hit
ATGGAAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA10.24509803921568626Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACTTTGGGAGGCTGAGGTGGGTGGATCATGAGGTCAGGAATGTGAGACC10.24509803921568626No Hit
CCATTAAGAAAGAGGAGGGCACCGGGCAGGCGATGCCACGGCAGAGCTGT10.24509803921568626No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTATACA10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
GGCCTGTGTGACCCAAAATTATTACACATGTATACACTCCTGTCTTTTAT10.24509803921568626No Hit
ACGCTGACCGCCGCATGCGCGGCCGCGGTGGCTCTTCAAGTGCGGCCTGC10.24509803921568626No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTGCGTTGATACCCCCCCA10.24509803921568626No Hit
CAGTGGTATCAACGCAGAGTAATGGGAAGCAGTGGTATCAACGCAGAGTA10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGA10.24509803921568626Clontech SMART CDS Primer II A (96% over 26bp)
GGGCTGTTCAGATGTTCTGTTTTTTTCTTCTTCTTCGTCTTCTTCGTCTT10.24509803921568626No Hit
TTCTTCTTCCATTCCTTCTTCCTTCCCTCCATCCTTTCTTCCTTCCCTCC10.24509803921568626No Hit
ATGCTGATGGGGGTAGGAAAGGTCCCCAGAAGCATGGGGGCTGAGTCAGT10.24509803921568626No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT10.24509803921568626No Hit
GTTTTCAAAAGTCCCTGAATTATTATTTGTCACTTCTCTAGAAATGAAGG10.24509803921568626No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTAATCTGAGT10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
CCCGAACTGGCAGATGTAGGGCAGCTGATCGCGGCAGCGCTTGTCGAACC10.24509803921568626No Hit
GGATCATACTGTGGGCCAATGCCATTTACTGCCTTGTGACGTTGGTCTTC10.24509803921568626No Hit
GATAAGTGGCTAATATATAAAATATGTAAAGAACAACTCAATAGCAAAAC10.24509803921568626No Hit
CAGCGGCCCTTCGCTCCACGCAGAAAACCACACTTCTCAAACCTTCACTC10.24509803921568626No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGTTC10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
GTTGGGTGGAGGTGACACTTCTCTGTAGTCCCCTATAATACCCCAAGAAC10.24509803921568626No Hit
AGTTAAGAGTGTTCAGATGCTCTCCTTGATTTTTTTTTGAGACAGGTTCT10.24509803921568626No Hit
GCTGTGTGTGAATGGGCGCGAGCGGACTCCCCTGCGGGGCGGACGCCTGA10.24509803921568626No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAACCGATTT10.24509803921568626No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTATTTTAATTAAGA10.24509803921568626No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.24509803921568626No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10.24509803921568626Clontech Universal Primer Mix Long (100% over 23bp)
ATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAA10.24509803921568626No Hit
ATATAGGGATTAAAGAAAGACATAGCAGCCATAAAAAAGAATGAAATCGT10.24509803921568626No Hit
GTGGGAAGCAGTAGTATCAACGAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.24509803921568626No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT10.24509803921568626No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTA10.24509803921568626Clontech SMART CDS Primer II A (100% over 26bp)
ACGCAGAGTACATGGGCAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10.24509803921568626Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGTACATGGGAAGCAGTGGTATAAACGCAGAGTACAAGGGAAGCAGTGG10.24509803921568626Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGATACCACTT10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
GTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTT10.24509803921568626No Hit
CCCGTGCTCTCCCCGCTAACCGTGTTGCCCCATGACCCGCTCAGAACTGA10.24509803921568626No Hit
CTCGTAAGAACGATCATGAGAATTCGGATCTCATTTTTAGACCTGTCTCT10.24509803921568626No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAGGTGGGTTTA10.24509803921568626No Hit
ATACAGGACTCGGCTCCGGGGGCAGGGCGAGGAATGGAAGTTGGAGTACG10.24509803921568626No Hit
TGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
GTACATTTATATGTCCCTGAGTCATAGATCCGGACTTCAGGAATAAAATA10.24509803921568626No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC10.24509803921568626Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGCTGGTGTGTGACCATCCTTTGGGGAAGGTCAAAGGGGGCGAGATCCC10.24509803921568626No Hit
GCATATACAAGACAAATGTGAAACATGACAATGAAAAAAAATCAATTTAA10.24509803921568626No Hit
CGCTTGAGGGCGTCCATGCGGAACGTGCAGCTCACCTCAGCTTGCTTGCA10.24509803921568626No Hit
GTCTTATATCAAACTTGTACAAAAGTTGAATCCATGGGGAAAAATCCAGA10.24509803921568626No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA10.24509803921568626Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTAAAACACAGCATCCCA10.24509803921568626No Hit
ATGGAAAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAA10.24509803921568626Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTACTAAAAAATACAAAAAATTATCCAGGCGTGGTAGTGGGCCTGTCTCT10.24509803921568626No Hit
ATGTAACAGGGTCCACAACAAATCCTGGCTTACATAAACCGAGTGTAAAA10.24509803921568626No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.24509803921568626Clontech Universal Primer Mix Long (95% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.24509803921568626No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGG10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
GTATTTCCATTTGGATGCCTAAGAGCCATCTTGTTGTTAACAGCCAAAGC10.24509803921568626No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATA10.24509803921568626No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.24509803921568626No Hit
CCCATGTACTCTGCGTTGATACCCGTGCTTCCCATGTACTCTGAGTTGAT10.24509803921568626No Hit
GAGGTGGAGGTTGTAGTGAGCAGAGATCATGCCACTGCACTCCAACCTGG10.24509803921568626No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTCTTAT10.24509803921568626Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGAAGATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCG10.24509803921568626No Hit
GATCAATTTGCAATTGTTTTCTTGACAGGTCGTTCCAGGGGTAGAGGAGG10.24509803921568626No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTTGTATA10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTG10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
TTGTGATGATCTGGAGGTATGTGCTGCTCATGCTGGCCGCAAGACTGTGA10.24509803921568626No Hit
GTATCTGAGCATGCTTACACAGAGGGAAACAAGCTAGAAAAAAATAAAGA10.24509803921568626No Hit
TACCAGACCACTCTGCTTCATTTTTGGGAGAAAACTTCTCACACTATGGC10.24509803921568626No Hit
CCTCTCTTATTTCCTTGAGCAGTGGTTTATAGTTCTCCTTGAAGAGGTCC10.24509803921568626No Hit
ATATAAAAATGTAAAAATCCAGCAAAACCAGAAATACGAATATATTTTTC10.24509803921568626No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTATA10.24509803921568626Clontech Universal Primer Mix Long (96% over 25bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCTG10.24509803921568626Clontech SMART CDS Primer II A (96% over 25bp)
CAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGT10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
GCATCGTCGACAGGCGCTCATTGAGCGACTGCAGGTAGTTCTCGAACTCG10.24509803921568626No Hit
GGTAGGGGCCGGCCAGGTCCCAGGTCTGGAAGAAGGGGCCCCAGTCGATG10.24509803921568626No Hit
GTACTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT10.24509803921568626Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTTACTTAAAGCATCTGTCCCCGGGAAGCTTTTGTATTTTGTACCCCT10.24509803921568626No Hit
GGTTCAGGTAAGGATGACTGATAGGAAATGTTGGTAGTTACGAGTCACAT10.24509803921568626No Hit
TACCACTGCTTCCCCCGTGTACTCTGCGTTGATACCACTGCTTCCCATGT10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTGGGATCTCAGCTTGCTGCAGCCTTGACTTTCCAGGCTCCAGTGGT10.24509803921568626No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATACACA10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACCTGTCTCTTATACA10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
ACATTGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC10.24509803921568626Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAG10.24509803921568626Clontech Universal Primer Mix Long (95% over 24bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTATA10.24509803921568626Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTCGTTTATAACACCCCA10.24509803921568626No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTTAAAAAGGGATAC10.24509803921568626No Hit
ATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAAT10.24509803921568626No Hit
ACTTGACAGACTTGTGTTTTATGGGCGGAGGTGGCGCCACCCGGGCCACC10.24509803921568626No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA10.24509803921568626Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTGCTTCCCATGTACTCTGCGTT10.24509803921568626Clontech SMART CDS Primer II A (96% over 25bp)
ACTTGACAGACTTGTGTTTTGTGGTATGTGCTGCCGGCCCCCGGGCCAAC10.24509803921568626No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC10.24509803921568626Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATTTATGTAATTCTTATCATCTAGGGTACTTTGTTTATTAGAGGAGAAA10.24509803921568626No Hit
ATTTCCAGCAAAGTGTTGAAGTATTTTGCCTGTTTTGTTTTTTTTTTTTT10.24509803921568626No Hit
CCTACTGGAGTTACTTATTAACATCAAGGCTGGAACCTATTTGCCTCAGT10.24509803921568626No Hit
GTACTTGCAAGGCTCCTAATTTCTTTAAGATTGCTGTATAATACCATTCA10.24509803921568626No Hit
GAATGTCCCTCGGTTTCAGTTTGTCTGATTTTGCATGATTAGAATGGGAT10.24509803921568626No Hit
GTGTATCAACGCAGAGTACATGGGAAGCAGTGTATCAACGCAGAGTACAT10.24509803921568626No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
GGACAGCCGGCTTGGCCCGGTCGGCGGTCAGGGGCATCAGACGCTTGCCC10.24509803921568626No Hit
CAAAGAGAGACCCCTGTCTCTACAAAAAATTTAAAAAAACTAGCTGGACG10.24509803921568626No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.24509803921568626No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATACA10.24509803921568626Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT10.24509803921568626Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGATACCACTGCTTCCCAAGTACTCTGCGTTGATACCACTGCTTCCATGT10.24509803921568626Clontech SMART CDS Primer II A (100% over 26bp)
TAGACCTACACAATACAATCTAAACTTAGCATGGCAAACAAAGATATTTA10.24509803921568626No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA10.24509803921568626Clontech Universal Primer Mix Long (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTATTTTTTTTATTTTTTTTTTTTT10.24509803921568626No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
ACTCATAACTTCCAAAATCTATTTTCTTCCTCACACCTGACACATATACT10.24509803921568626No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACTCTGCGTTTATACCACTG10.24509803921568626Clontech SMART CDS Primer II A (95% over 23bp)
TCAATATCTAACACGAGGATGTTCCTGTTGATACCGTCTGCGATAGGCTA10.24509803921568626No Hit
GCCTTTCAGTGACGCGGTAATATTTTACGATAGGTGCCGATTCAAATCAG10.24509803921568626No Hit
GGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTCTGT10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
CATTTCTTTGTGTCCCCATGGCATCTGGCACAGCACTTTTCACTCCAAAG10.24509803921568626No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT10.24509803921568626No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10.24509803921568626No Hit
CAGATATGTTAAATCAACAAAACTGCTCGCCAGAACACTACGAGCCACAG10.24509803921568626No Hit
ACATGGGAAGCAGTGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA10.24509803921568626No Hit
GTTTTGTGTATGTGTGCTTTTTTAAGTTCATCGTAGGTGGCTTAACTGCA10.24509803921568626No Hit
TATTTAAATTTCCCAGAATTTTTTTTTCTTTTTTTGGGACGGAGTCTCGC10.24509803921568626No Hit
TCCCAGGACCCATCAGCAGGGATGTGGGATGGAGCAGGGAAGAGAAGGGA10.24509803921568626No Hit
GGCGGGTTCAGGCTTCGGGGGCCAGCCGCCGCCATGATCCTGCTGGAGGT10.24509803921568626No Hit
GCTGCTGGATTCGGTTTGCCAGTATTTTATTGAGGATTTTTGCATCAATG10.24509803921568626No Hit
CAAATAAGCCCATCTCTAGGTGCTAGTGTCCAGGACAACTGCTGTATTCC10.24509803921568626No Hit
GAGTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAATTAA10.24509803921568626Clontech Universal Primer Mix Long (95% over 21bp)
CCCTCAGGAATGTGGAGAAAGTGTGGAAGGCCGAGCAGAAGCATGAGGCT10.24509803921568626No Hit
CCCTCGAAGTGGACTACGAGTGCGAACTCGCCGTCGTCATCGGCCGCGCC10.24509803921568626No Hit
GCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT10.24509803921568626Clontech Universal Primer Mix Long (96% over 25bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAAAACAGTGTAAC10.24509803921568626No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA10.24509803921568626Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATAACACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTTAACA10.24509803921568626No Hit
ACGGTCTCATGGAATCCAGTGGAATCCATGCTTGGACTTTGGGAATGACA10.24509803921568626No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.24509803921568626No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATA10.24509803921568626No Hit
ATCAAGCAGAGAGGCGGCGGCAAGATTATCGGCGCCGCATCCGTTGTGGC10.24509803921568626No Hit
GCGCCGATGGGCATGGCCGAGATCGGTGTGGCGCTGTGGGCCCGGCATCT10.24509803921568626No Hit
CTTATGCCCCAACCCCTCCCAATCAAAACAAAACACAACCCTGAAAACAA10.24509803921568626No Hit
ATTTATAACTTAGTACATATTAGTGTTAGCCAAATCCCATAGAAATCCTT10.24509803921568626No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAC10.24509803921568626Clontech Universal Primer Mix Long (96% over 26bp)
GTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGAGGGTCGAGT10.24509803921568626No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)