FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003635261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003635261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCAGAGGCACGTCATGTACCTCGCTGCTCGATGCGCGCCGTCCATACA13.8461538461538463No Hit
TCTGGGAGCTGGCCAGACTTGAAGGGTAGTCCTCACCATCCCTCCAGTCA13.8461538461538463No Hit
CCTAACTGCTCTATGAAAAGAAAAGTTAAACTCTGTGAGTTGAACGCACA13.8461538461538463No Hit
CATGAGCTTCCAGTGTAAAAGAGGCCACATCTGTAAGGCAGACCAACAGG13.8461538461538463No Hit
ATCAACGCAGAGTACATGGGAGTATATGAATCATATCTTGGGGTTAGATA13.8461538461538463No Hit
CGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGA13.8461538461538463No Hit
TCCCTACAGAAGATGCTGTATTTTTCAGCACTATCTGTTCTCTACATTGA13.8461538461538463No Hit
CTCTTTTTGTGGAATCTGAAAGTGGATATTTGGATAGCTTTGCGGATTTC13.8461538461538463No Hit
CACTAAAGGGAAGCATCACCCCTGACCTGTGATCTTGACATTGCAGGCTC13.8461538461538463No Hit
CTATTACCCTCTGGATGTGGTGGTGACGTGGACCCGAGAGGAGCTGGGTG13.8461538461538463No Hit
ATCAACGCAGAGTGGAGTATTTTTTCATGTTTCATGTTTGTTGACCACTT13.8461538461538463No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG13.8461538461538463Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAAAGATTGCCATGACAAGTGAGCTGGGCCAGCTTGCTAGATAAATTCA13.8461538461538463No Hit
AAATTAAAGGGAAAGTCTTTTATTTAACTTATTCTATTTTTATATCTTTC13.8461538461538463No Hit
GATTTTCATGCTGTGGTCACGAGGCGAAGACGGCGGAGTGGCGGCCGGGG13.8461538461538463No Hit
AGAGAAATACGGCAGAGAGCCCAAGACCATGAAGCCAAGGCTATTAGCCA13.8461538461538463No Hit
AGTCTTTTTTGTAGAATCTGCAAATGTATATTCGGATCGCTTTGAGGCCT13.8461538461538463No Hit
TCGTCGCCCGCATCGACAAGCGCCTGCACCGCCAGCATCAGCGCGCTCAC13.8461538461538463No Hit
GTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCA13.8461538461538463No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG13.8461538461538463No Hit
TTATAGTCCTAAGAATCCAAATGCTGGTAAAAAACTTTCTGATGGATTTG13.8461538461538463No Hit
AGTTCAGTATTTCTCCCTTCCTGTCTCCAGTGTCTGCAACAGCAAATCCT13.8461538461538463No Hit
CTAAATCTTAAACACATCTAACAACCCACAATACATAAGAAAAACTATAA13.8461538461538463No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATACACA13.8461538461538463No Hit
ACCCTGTCTCTACTGAAAATACAAAAATCAGCCAGGCTTGGTGGCATGCG13.8461538461538463No Hit
CATGGCTATACAATACTTCGTAGACACTTGGACCATATCTTGACCGTGAA13.8461538461538463No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)