FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003635262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003635262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCCTGTCTCTTATACACA13.8461538461538463No Hit
ATATTTTGGGCATCATCAGGAAAGGGAGAGTAGTTAAGCCTTTCATATAA13.8461538461538463No Hit
CATCCCAAAGAGATATCTCAGAATGCTTCTGTCTTTTTTTTTATGTGAAG13.8461538461538463No Hit
GAATTGCTTGACTTAGCAAAGTTTCATTTCGGTGACTCTTTATGCACACA13.8461538461538463No Hit
GCATAGGTCTGATTGTCAGTGCCACAAACTTGATCAAGGGGTTTTGTTGG13.8461538461538463No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAGTGTAGTCGTGGCCGTCCATGTAC13.8461538461538463Illumina Single End PCR Primer 1 (96% over 26bp)
ACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCA13.8461538461538463No Hit
GGTTCGGCGAGAGCGACGAAGTCACCCCCGAGGTGGTCCGCCAGGCGGCC13.8461538461538463No Hit
GGGCTAGACCGTGGCGCCGGCAGCGGCCCCTGGGCTGGAGGAGGATGATG13.8461538461538463No Hit
GAAAGAGGGAATGCATGCTTCTGTGGAATTTCATGTATAGATATTTGTGT13.8461538461538463No Hit
GACTACTGCTGCAGACTGCAAGCAAGCTGTCAATAGGCTCTATGCAAGGC13.8461538461538463No Hit
ATATTTTGTGCTTTGGTGTGTCTTTCCATTTCTATCATTCCCCACACCTC13.8461538461538463No Hit
TTAGTAGTCCATCCTGATGTTGTTGAGGCTGCATAGACACGACCGTATTG13.8461538461538463No Hit
GTCTTTGGGTTCCGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAA13.8461538461538463No Hit
ACCATCCTGGCTGATACGGTGAAATCCCGTTTCTACTAAAAATAAAACAA13.8461538461538463No Hit
ACATAATTAAATGGAACTTTAAGCAATTATTACAGTTCATCTCCATGAGG13.8461538461538463No Hit
CTTCAGCGGCATGCGCGAAAAATCGCGCCGCACAATAGACGATACGGCGC13.8461538461538463No Hit
ATTACATTTAAAGAAAGGGAAGAAATCTGTTTACTAAATGTTAGACTTGA13.8461538461538463No Hit
ACTCTGTGAGTTGAATACACACAACACAAGGAAGTTACTGAGAATTCTTC13.8461538461538463No Hit
CCTAATGAGTGTCCAAGCTGGGATTCAAACCCAGGCCTGTCTGATCACCC13.8461538461538463No Hit
GTTCTGTGACTTGAATGCAAACATCACAAAGAAGTTCCTGAGAATGCTTC13.8461538461538463No Hit
CTGCGCCACTGCACTCCAGCCTGGGTGACAGTGAGACTTTGTGTCAAAAA13.8461538461538463No Hit
CACGGAGTCTTGCTCTTGTCACCCAGGCTGGAGTGCAATGGTGCCATCTC13.8461538461538463No Hit
TGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA13.8461538461538463Clontech Universal Primer Mix Long (95% over 21bp)
GACAGATACCAAGGGGAACCCGCACCGTGCAGACCACCGACCACCCGAGT13.8461538461538463No Hit
AAGGAAGAGACTGCTGGCAAAGATGACTCCCAAGGCTGTTCTCCGCTCTG13.8461538461538463No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)