FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003635313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003635313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46
Sequences flagged as poor quality0
Sequence length100
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAACTAGCGCGACCCATCTGGTTCGGAGAAGGCTCACGTCCTGATTCT36.521739130434782No Hit
GCTTACCTGTGGTGGTTTTTGCTGGGCTTCCTGGGGGCGCATCGCTTCTA36.521739130434782No Hit
TTCTGGGACTGCTCGACTGCTTTGAGTAGCCGCACGTATTGCGTTAGATC24.3478260869565215No Hit
GCTCAGCCCCATGACGGCTTGGGCCCAGGCAACTGCCAAGACCTTCACCA24.3478260869565215No Hit
GCGGTGCTCACGCGCGAAGACGCGCCCGACGACACCGCCGCAGCGCCCGT24.3478260869565215No Hit
GCCATCTACCGACAGAGCGCGTCGCGGGCATGGATTGCGAAGTCTTCCAG12.1739130434782608No Hit
GGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTA12.1739130434782608No Hit
GTTTTCCAGCATGTACGACACACCGGAAGGCACGCGCAGGTTGTCTTCCA12.1739130434782608No Hit
CACGTAAGGCTTGGACTTGTAGCCTTCCTTCTCGAACGCACGGCGCATCT12.1739130434782608No Hit
GTATCAACGCAGAGTACATGGGCAAGCAGAAGACGGCATACGAGATCTTG12.1739130434782608Illumina PCR Primer Index 1 (96% over 28bp)
TTTTATCACCGCGCCTGTGCGGCAAACGCCGCGCGGCCACGGATTTACGC12.1739130434782608No Hit
ATGACAAGGAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTC12.1739130434782608No Hit
GTCCTGGACCTTGTACTCCGCAGTCATCCTGGTTCCTTTGATGATGCGAG12.1739130434782608No Hit
CATCGGGAATCATCCGAAAAGGTGCGGAATCGTCTTCAACCTGATCGTGA12.1739130434782608No Hit
ATCCAGTGCCATCAGCGGGTGATTTGTGTGGTGCATATGATCAGGCGGCA12.1739130434782608No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG12.1739130434782608Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACAGGGCTGGGTTCGGATGCGGCGGGCAATGCCGTCGCCAAGCCCTTT12.1739130434782608No Hit
ATCATCCTGGATCTGGACCGCGACGTCTGGGGCTACATCTCGCAAGGCTA12.1739130434782608No Hit
GCGAGGCGCTCGACGACGCCGCCGGCGAAGCCTTCGACAAGACCGCCAAG12.1739130434782608No Hit
GCGAGACGCTCGACGACGCCGCCGGCGAAGCCTTCGACAAGACCGCCAAG12.1739130434782608No Hit
CTCCTACATTTGCTATGCGTCTCAAGATAAGGATATTATCTCATATGGCG12.1739130434782608No Hit
GTGCATCGTAGTGGCGCTCTACGGGCTGCAGATGCTGCCGATCAACTACG12.1739130434782608No Hit
GATCGCGCTCGTGCTTCCTGGTGTGCGCCGCAAGGTGTTCGGCAAGTTAG12.1739130434782608No Hit
GTGTGGTGCTTGGCATTGCACTGGGCGGCTTCTGGACCATGGCAACCGCC12.1739130434782608No Hit
GTACAGGGCTGGGTTCGGATGCGGCGGGCAATGCCGTCGCCAAGCCCTTG12.1739130434782608No Hit
CTCGGACGGCGATAGTAGTAGATACCTTCGGACATTTCGCGCTCGATCAG12.1739130434782608No Hit
CTGTTTGTCGCGCACGACGGTCGTGCCGTACAGCTTGGCGAGCCACTCGA12.1739130434782608No Hit
GTGGCGGCCAATGATGCCAAGCAGGATCCGCTCGACTTCGTGCTGTGGAA12.1739130434782608No Hit
GTGCCGGTTCGATTGATCTTTTCGGACAGGCGCTCCATGGCTGTTGGCGA12.1739130434782608No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTGGTTATAAA12.1739130434782608No Hit
GTGCTGCGGTGCGCCACGCTGACAATCGTCACATCAGGCATCGATTCGAG12.1739130434782608No Hit
GGTGCAAGCCGATACGTTCGACAAGCCGTTCGACATCAACGAGGGCGTCA12.1739130434782608No Hit
CAAACCAAAATAAAATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACT12.1739130434782608No Hit
GTTCGCCGTTGAGCAGCTCATCGCGCAGCTTGCCGTTGAAGGACTCGCAG12.1739130434782608No Hit
GTTGCGCCCATGCCGACGCCGATGCGCGCCTCGTTCATCATGTGGAACAT12.1739130434782608No Hit
CGCCAGCAGCGCCCCGCCGTCAAAGAACGTCTGTGCCACGTTGCCGACCA12.1739130434782608No Hit
GGGCGGCTATGCCAGCCTGGAGGGCGGCAGCGATACGGAAACCGATAGCG12.1739130434782608No Hit
GCCCGTCGGCGCGGCCGCTGCATGGCTTGAGGCCGCTGGGCACCAGGGCC12.1739130434782608No Hit
ACGCAGAGTACATGGGCAAGCAGAAGACGGCATACGAGATCTTGCTTAGT12.1739130434782608Illumina PCR Primer Index 1 (96% over 28bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)