FastQCFastQC Report
Fri 10 May 2024
EGAF00003635314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003635314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46
Total Bases4.6 kbp
Sequences flagged as poor quality0
Sequence length100
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGTATTGATCGCGCGAAGTCGTGCACCCTGCCAACGCGCCAGCTGCG36.521739130434782No Hit
GGGTCGGGATGGCCTTGGGCGAGGTGAACGAGGTGACTTCGATCTTGCTC36.521739130434782No Hit
GGTCAGCACCACCACGGCGGCAAACGGGCCGACACCAGGGCCGGGTTTGA24.3478260869565215No Hit
GCTCGCGCGCGGCGCGCAGCGTCTTCTTGACCAGCACTTCGACGATAGCG24.3478260869565215No Hit
ACCACCGATGCCGCTTCGGCACCGAAGGCTGAAGCCAAGCACGAGAAGAA24.3478260869565215No Hit
GTCCGACAGGTGGAACGCACCTTCTTCAATCGGCACCATGCGTGCGTCGA24.3478260869565215No Hit
CAGGTTCTCTTCGGAGACGGGGCTTTTCTCATTGGCCCGATTTTCTATGT24.3478260869565215No Hit
GTCCACGGCAAGGAAGCTTTTCCCCCTCGCGGAGAAGAGCATAGACGAGG12.1739130434782608No Hit
TAAGACCACGGGGCCAAACGATTGTGCATTATTTGCGCGATCCGACTGGG12.1739130434782608No Hit
GGATGAAGAAACGCACGACTTCCGCGTCGTACAGCTTCAGCACATCGCGG12.1739130434782608No Hit
GCCGATCGTCCAGCCTTGCTCGGCCAGATTGGTCGGCTGCGTCACGATCT12.1739130434782608No Hit
TGCGTGTCGTCCAGGTTGTCCGTCACTTCCTTGCGGTACCGCGCGATGAA12.1739130434782608No Hit
TTCTGTGCCTTAGGAGCGGATGTTATTGAGAAGCACTTTACCTTAGATAA12.1739130434782608No Hit
AGCCAGCCGATTCCCGTGAAGTGACGCGCAAATCTGGCCGCTAAAGCGAT12.1739130434782608No Hit
CGTCGTCCCGCTTGGCGCCATCGATGGCAGCGTGGCGACTTGCGATACGA12.1739130434782608No Hit
AGCGAAGGCATCCCCGAGACCTTCACGTTTGCGGACTTCACGCTGCCGAA12.1739130434782608No Hit
GCGCCACGCTCGTCGCCATCGATCCTTATCGCTCGCTGACGGCCGAGAAG12.1739130434782608No Hit
GGTGGCAAACCATTCGCCGGCGTTACCGGAGTATTTGCGCTCCATCGATG12.1739130434782608No Hit
CACCACCGGCCACGCACAGGTAGTAAAGGATGTTGCCCGGCAGCGATAGC12.1739130434782608No Hit
CGTCTGAACTCCAGTCACGAAGCAAGATCTCGTATGCCGTCTTCTGCTTG12.1739130434782608Illumina PCR Primer Index 1 (96% over 28bp)
GTCCACTACTTCGCCTGTCATGCCGATGAGCGTATCGCCGCCGGTCACGA12.1739130434782608No Hit
CGCCATGGGCGAGCGCGTTGCCAACGACGAAGCCAAGATCGACGAGTACA12.1739130434782608No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCATCTCCTCTAACTTTGGAGA12.1739130434782608No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAGGACCTGTTGGTA12.1739130434782608No Hit
GTTCGGCCGCGAGCGCCCGTTCCACAACGCGCCGCGCACGCTCGACCTGG12.1739130434782608No Hit
CGCCACTTCTTTGAATACCTGCTGCCGGAAGCGGCGCGCAGCACGCCCAA12.1739130434782608No Hit
GTCAGGCGATTTTCCAGGCCGAGGTCCAGCCGGCGCAGTTGCTCGCGGAA12.1739130434782608No Hit
CGTCTGGAGCTACGACTTCGTGCACCACACTACCGAAGACGGCCGCAGCC12.1739130434782608No Hit
GGGTAGCGTGTTCTGACATGGATACAGAGTGGTCGATTTTGCTCGGATCG12.1739130434782608No Hit
GCGCAGGCCCGTGCGGCCGAGCGCTTTCTCCTGATATTCCTGGTAGGCGC12.1739130434782608No Hit
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA12.1739130434782608No Hit
ATCGTCCACCTCGTGCTGGCAAAGATTCCCGGGCCTGACGGCAAGCTGAT12.1739130434782608No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT12.1739130434782608No Hit
CTTTTGCACGTGCGTTGCTGCAGCGCCCGGATTACCTCTTCCTCGACGAG12.1739130434782608No Hit
GGGCCAACTGCCATGTTGTGTCCAGTCGCTATCGGAGTAGCCGCGCTGGT12.1739130434782608No Hit
GAGTACATGGGTGTAGCCCACCGAGTCTACACCTTTCCTACACAGCAACT12.1739130434782608No Hit
CTTCCGCCGCGTCGGTATCACCTTTGCTGTGTACGGCGACAAGGACGAAT12.1739130434782608No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)