FastQCFastQC Report
Tue 10 May 2022
EGAF00003635321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003635321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106054
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGTTCACCCGCATCGCGTATG19541.8424576159315067No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCACCCGCATCTCGTATG17241.6255869651309711No Hit
ATACACATCTCCGAGCCCACGAGACCGGTTCACCCGCATCGCGTATGCCG11811.1135836460670978No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA10831.0211778905086089No Hit
ATACACATCTCCGAGCCCACGAGACCGGTTCACCCGCATCTCGTATGCCG9330.8797405095517378No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7870.74207479208705No Hit
ACACATCTCCGAGCCCACGAGACCGGTTCACCCGCATCGCGTATGCCGTC7510.7081298206574009No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA6910.6515548682746526No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6790.6402398777981029No Hit
ACACATCTCCGAGCCCACGAGACCGGTTCACCCGCATCTCGTATGCCGTC6160.580836177796217No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA6020.5676353555735757Clontech Universal Primer Mix Long (95% over 21bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA5300.49974541271427764Clontech Universal Primer Mix Long (95% over 21bp)
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA4540.42808380636279636No Hit
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAA3760.3545363682652234No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3420.32247722858166594No Hit
CATCTCCGAGCCCACGAGACCGGTTCACCCGCATCGCGTATGCCGTCTTC3420.32247722858166594TruSeq Adapter, Index 18 (95% over 22bp)
TCTCCGAGCCCACGAGACCGGTTCACCCGCATCGCGTATGCCGTCTTCTG3330.31399098572425366TruSeq Adapter, Index 18 (95% over 24bp)
TCTCCGAGCCCACGAGACCGGTTCACCCGCATCTCGTATGCCGTCTTCTG3180.29984724762856657TruSeq Adapter, Index 18 (100% over 24bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA3060.2885322571520169Clontech Universal Primer Mix Long (95% over 21bp)
CATCTCCGAGCCCACGAGACCGGTTCACCCGCATCTCGTATGCCGTCTTC3030.2857035095328795RNA PCR Primer, Index 18 (95% over 23bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA2950.27816018254851305Clontech Universal Primer Mix Long (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2520.23761480000754334No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA2370.22347106191185623No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2360.22252814603881044No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT2250.21215607143530657Clontech SMART CDS Primer II A (100% over 26bp)
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA2030.1914119222282988No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2030.1914119222282988No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA2010.18952609048220717No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1790.1687819412751994No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT1630.1536952873064665Clontech SMART CDS Primer II A (100% over 26bp)
CACATCTCCGAGCCCACGAGACCGGTTCACCCGCATCGCGTATGCCGTCT1570.14803779206819168No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1560.14709487619514586No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCACCCGCATCGCGGATG1490.1404944650838252No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA1430.13483696984555038No Hit
GTACATGGGCAAGCAGAAGACGGCATACGAGATCTTGCTTCGTGACTGGA1430.13483696984555038Illumina PCR Primer Index 1 (96% over 28bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA1400.13200822222641295Clontech Universal Primer Mix Long (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT1400.13200822222641295No Hit
CACATCTCCGAGCCCACGAGACCGGTTCACCCGCATCTCGTATGCCGTCT1380.13012239048032134TruSeq Adapter, Index 18 (95% over 21bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA1340.12635072698813812No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT1340.12635072698813812Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1320.12446489524204651No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA1300.12257906349595489No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA1300.12257906349595489No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1280.12069323174986328No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT1280.12069323174986328No Hit
ATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA1210.11409282063854263No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1210.11409282063854263Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1180.11126407301940522No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1130.10654949365417618No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT1110.10466366190808457Clontech SMART CDS Primer II A (100% over 26bp)
GTACATGGGAATGATACGGCGACCACCGAGATCTACACCGGGCTCGGAAA1110.10466366190808457Illumina Single End PCR Primer 1 (96% over 29bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA1100.10372074603503877Clontech Universal Primer Mix Long (95% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1080.10183491428894713No Hit
GTATCAACGCAGAGTACATGGGCAAGCAGAAGACGGCATACGAGATCTTG1070.10089199841590132Illumina PCR Primer Index 1 (96% over 28bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)