FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003635355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003635355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41
Sequences flagged as poor quality0
Sequence length100
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGATGAAGGCCCTCGGGTTGTAAACTGCTTTTGTACGGAACGAAAAGC49.75609756097561No Hit
CTCAATACATCGACCGAAGCCTACTCGCCGTCCTCGGTCCTGCGGACCGG37.317073170731707No Hit
GCCGTCAGGATTTCCCTGATAGGCAGATCGTCCCGAATCACCGCTTCACC37.317073170731707No Hit
CCGCCATCCATAAAAATGGTTGTACCAGTTATGTAATCTGCCTGGTCTGA24.878048780487805No Hit
TCGTGGAACTGGCCCGGGCCGGGAGCCTGCGGGCGGCCGCCGCCGGGCTG24.878048780487805No Hit
TCGTAGAAGTCACCTCCATAGCCTGGAAGCTCAACTTGATCATCCGGAAA24.878048780487805No Hit
GAGTGCGCCGACCTCCACCTCCGGGTGGGCCGTGAGCAGGCGCAGAACCT24.878048780487805No Hit
CAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCTGC24.878048780487805No Hit
GCAGACAACAGTGTTGCTGGTGGGCTCGATGGCGATGCCATCACCGACGA24.878048780487805No Hit
GACCACCTCGACGGCGGCCTTGTCGACCGCGGCGATCCCGCGATCGAGCA12.4390243902439024No Hit
GCTGCGGACTCCTTGCGGACTATCCGCGGACTCGCTGGGGCCAGGCAACC12.4390243902439024No Hit
GAACGAGGTCGATCATTCATCTGCTTTCTTAAATCTTTACGATCAATACC12.4390243902439024No Hit
ACCCGATCCCACCATGGAGCAAGCCTTTATTCAGTTGATCCACGACTGGG12.4390243902439024No Hit
GGATCAGCCGGGCAATCAGGTCGCGCTGACTGACCTTGTCCTTGGCCCGC12.4390243902439024No Hit
CAAGGAAATTAAATAAGGCAAAATTCTATTTAAAAATGAAAGATGAGACA12.4390243902439024No Hit
GTGCGACACGACCCGTGCGGGCGTCGCCGTTAGTGTGGACCGATGGTGGA12.4390243902439024No Hit
TCGTGGGCGCGTTCTACATCAAGAAGGAGAACTACTCCCCCTTCATCCCG12.4390243902439024No Hit
CAAATCAGTGTGCTCATCCAGTTCAATGGTCGCGATAACGCCATCTGAAC12.4390243902439024No Hit
GCGCGACATCTACGCCAATCCCCAATTGGCGACCAACCGGCTGTTTACCC12.4390243902439024No Hit
GAATGAGAGAGTTCTTATTAAGGTCAGTGCTCTAATATTCATGTCTTGAT12.4390243902439024No Hit
CACCTGAGCTGCGCCTTTTTCCTTTGCCAGCAGACTTTTCGCCCTCCATC12.4390243902439024No Hit
TCATCTTTCCTATACCCTTCAAAGACTAGAGACTTGTCAGATTCCATTAG12.4390243902439024No Hit
CCCCTGTGCATGCGGAAATCGTTCCAGCCCACAACACAAACAAAAACCCA12.4390243902439024No Hit
GACCAGAACATTCCCATCGTCGGGGTCATCACCCACGCCTTCGAGGGCGA12.4390243902439024No Hit
CACACGTAGTCAACGTGGCAAGGCGTGTAATTGCTGCATTTGCAATTAAG12.4390243902439024No Hit
TATGCGTAACAACCCACTTCGTCGGGTCAGGAAGGTTGAGGATAAGCAAC12.4390243902439024No Hit
ATCCCGGACTCCACGGTGCTCCGCGACGGGGACGTGGTCAACGTCGACAT12.4390243902439024No Hit
TGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAG12.4390243902439024No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)