FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003635356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003635356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41
Sequences flagged as poor quality0
Sequence length100
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATGGCAGTTCCCAGGTTGAGCCCGGGGATTTCACCACTGTCTTACAAA49.75609756097561No Hit
CCTAAGCGGGCTCCCGGCACAGTTCACTCGGAACCTCGGCGAGCGTCGTC37.317073170731707No Hit
TCGTTGCCGGGGTGGCTTGAAAGCGCATGCGCCTTCGTTGGTCGGCCAGG37.317073170731707No Hit
ATCCATAACCGAGACCGCAACAGTCGAGGGGGCCGAGGCCCCGACTGCAG24.878048780487805No Hit
CCCCGCTGCTTGTGGTACAGCCCCACCCCGAGCTGCGCCTCCAGGGCGAC24.878048780487805No Hit
CCACATGACCTTCGCGGGCCGCTTCGACCCCTCCAAGCTCAGTCTGCGGG24.878048780487805No Hit
GATGATATTGGCAAACTGGCCGCCTGGCTGGCATCAGACCAGGCAGATTA24.878048780487805No Hit
CATCAGGTACGCGACAGCTACCATCAAAACCTGTTATGGATAGGCCATCA24.878048780487805No Hit
GCACCGGCGGTGCAAGCTGGATATTCGGATATGCGCGATCTCTATCCTGT24.878048780487805No Hit
GTATAGTGGATATCAAGGGCCGTGAAGGCGCCTTCGTAAAGCGCACCGAG12.4390243902439024No Hit
CTGCGGGCCAAGGACAAGGTCAGTCAGCGCGACCTGATTGCCCGGCTGAT12.4390243902439024No Hit
GCCTAAGAGTCCCTCGACTCGAGAATACAAACATGTTGCCTTCAATACAC12.4390243902439024No Hit
AACTCAGCGGGGTCGGCCCGCTGTCACCCGAGCACGCGCACCGCGAGCTC12.4390243902439024No Hit
GTACAAGCGTGACGGCAACGTAGAGAACCGTGACGATGGCGAGCGAGCCG12.4390243902439024No Hit
CATGAAGAGCCATCCACCCGCATTCATCTGCGCGCACCACAGACCCATCG12.4390243902439024No Hit
CTTAATGTCTGTCTCAATGCTTTGTGACTTAAACATGATTGTGCTATTCG12.4390243902439024No Hit
ACTGCAAGCTCCGCCTCCTGGGTTAACGCCATTCTCCTGCCTGAGCCTCC12.4390243902439024No Hit
ATCCTGTGTGAACGCGCTCACAATGCAGGGCAAGTATTAAAAAAACCATA12.4390243902439024No Hit
GGCCAGGGTGCCGCCGTGCACCGCCACGCCCATCCGCTCCACCATCGGTC12.4390243902439024No Hit
CCTTTGGCGATCAGTACACCCATCATGAAAATTGTGATTGATCCACGTGA12.4390243902439024No Hit
GTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCATGGTGTGGG12.4390243902439024No Hit
GCAGTTTCTCTTCAGAAGGTGAAAGTTGGCGGAAAAAGAGCCGTTCCCGC12.4390243902439024No Hit
TGCCTGGTCGATGAAGGTAACTCCGTCGGCACCACTCTTCTCGACACTCC12.4390243902439024No Hit
AGTCAATGTAACACATGCATGCAAAATTGCAAAGCTGCAATTACACGCCT12.4390243902439024No Hit
CGGTGATGGTGTTTATACCCGATCAGAAACGACAAATCGTGGCGCAGTTC12.4390243902439024No Hit
CCGCTCGATGACCCGCCCGATCACGTTGACCGCGCGCCCGGGCATCACGG12.4390243902439024No Hit
AACCACAATATCTCCCCGCGCTTTATGACGGAATGGGATATTTTTGCTAA12.4390243902439024No Hit
GGGCGGACCGAACGGATGGAGGGCGAAAAGTCTGCTGGCAAAGGAAAAAG12.4390243902439024No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)