FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003635366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003635366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141
Sequences flagged as poor quality0
Sequence length100
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGAATCCCCCTTACCAGGTGCGCGCCGGGCTGGCCCGGTAGCTGCCCT64.25531914893617No Hit
GTGTGGACCTGCCGGGCTTCGCCTCGCTGGGGATCGGTCGGGACGTCGTC53.546099290780142No Hit
TCCTGGTGCTGTGCTGGTCTTGGAGCCAATGGACTTGGGGAGCATATCAC42.8368794326241136No Hit
GTTCTCTTCACTTTAAAAATAACAAGTGGAGGCTCAGGGGAGTCATTTTC32.127659574468085No Hit
GTACATCATCGCGCCACCCATCACGCGAACTGCCGGCACACCTCCAGCGT21.4184397163120568No Hit
GCGCTTCATGGTGCGGCTGGTGAAGGGCGCCTACTGGGACGGCGAGATCA21.4184397163120568No Hit
ATGGTGCGCAGCGATTGCACCATCTTCTGCATGGACTGCATCAGCTGGCC21.4184397163120568No Hit
GGCGTACTATCCACTTCGGTGTTCGCGAGCACGCCATGGGTGGCATCCTC21.4184397163120568No Hit
TCATCGCCACGCGCGACGCGACCATCGGCATGGGCGGCCCCGCCATGATC21.4184397163120568No Hit
GTCAAAACCTTTACCGATATGCAGCAGGTTGACCACGACGCGCTGGTGCA21.4184397163120568No Hit
CTACTTCCCCTTCCAGATTGGGGCTGTTTCAGTGCCAGGCTTGGGAACGC21.4184397163120568No Hit
CTTTGAGGTCCTTAAAAGGTTTCTGTGAAAATGAACGACTGAATCCTCAG21.4184397163120568No Hit
GCCCTCAGCCACGGCACGCTCCTTGGCGATCAGGTCGGCAGGCACGTCGG21.4184397163120568No Hit
GAATGTCTGCCGCGTCGGCCATCAATGCCGGCGAACGTGTTTTGTGGCCG21.4184397163120568No Hit
GATGGGGTTTCGCCATGTTGGCCAGGCTAGTCTTGAACTCCTGACCTCAG21.4184397163120568No Hit
GCCCAATTGCCTCTGCAATGCAATGTTCTCGCGCTCCAGTTCTCGCGCCA21.4184397163120568No Hit
GCATTGGGACAAGGCGACGCACAAGTCGGTCGAACTGGATGGCCGCACGC21.4184397163120568No Hit
TCGAAGCCCATCGACGAGAAGATAGCCTCGATGCGCTCCATCGCGCGGGT21.4184397163120568No Hit
GGACGATGTTGGTCGCGGCGGCGCGGGCGCGACGCAGGTCCTTGTGCGGG21.4184397163120568No Hit
GATCACGCCCGAATCTTCATTGTTGAGCAGCCACTGGTAGAGGCCCTGGA21.4184397163120568No Hit
GTTGAATACGCGTGGAACGATGCAGCGGCGATTGCGGAGCAGCGGGGCCG21.4184397163120568No Hit
CCGCCGTTCTATTGAGGAGACCGAGAGGGCAGGGGCCTTGCCGCCCGGCG21.4184397163120568No Hit
CTGTGACGCTGTACGGCCAGGTGGATGCCTGGGCGGGCGCCACCAAGAAC21.4184397163120568No Hit
ATGGTGATGATGGCAGCGATGGTGATGATGATAGCACCAATGGTGATGAT21.4184397163120568No Hit
ATGGTACATGCTATGGTACACAGACTTACTTATTTGCACTTTACTTGGTT21.4184397163120568No Hit
GTCGATGTGAAGGCCAACCCACGCCTAGGGTCCTGGGACGCTAACAACCT10.7092198581560284No Hit
CAAAAAGACTGCCATATGGTAAGTACTCAACAAATGGTTTACAAATAAGT10.7092198581560284No Hit
GTTAAAATATCTTGGCCTAATAAGGGGACTGGGCAGGTGGGGATAACCAG10.7092198581560284No Hit
GCGGCAACGTGATCTTCGGCATGTTCCGCGTGAACGCCGGCTACTTCCAC10.7092198581560284No Hit
CGTGCCGGGATTCGAAATGATAAGGGTTGCAGTCGACACCTTGGCAGGAA10.7092198581560284No Hit
GTTAGAGGACAGCCTGGGAAACATAGCAAGATCCCTATCTCTACAAAATA10.7092198581560284No Hit
GCATGAATGAGCCGATGAAGGGGAGCAGAAGATAGCGGCTGACGGGATTG10.7092198581560284No Hit
GTTTCCTTTACAGCCTGCAGTCATTAGGGGACACAGTCAAAAAGGGAAGC10.7092198581560284No Hit
GCGTGGCGGCGGCGGGCGCGGTCGTCACGGTGGTGCCAGGGCTGGCGCAC10.7092198581560284No Hit
CTTTTGATTCTTTTTCAATGATTTAAAAATGTGAAAGCTATTCTTAGTTC10.7092198581560284No Hit
GCGTACTACCGAGCGGCTGCGCATTCATGCCCGTTCTCTGGGCATTACTT10.7092198581560284No Hit
GTGAACAGCTTGCGTCGCAGCTCGACGATGGCCAACGCGATATTGGGCCG10.7092198581560284No Hit
GCCATCGCGCTCGCGGCGATCTTCGGCTTCGCGTACCTGACGTTCCGGCG10.7092198581560284No Hit
TGTCAAAGGGATATTCTCTACTTGGGTAGCAGTTGCCTAGAAGACTAAAT10.7092198581560284No Hit
GTACTGGGCGATGGGCATCGGTGGGGTGGTGGCGGCTGTTGCGGCTGGGG10.7092198581560284No Hit
GCTGGCTGCTGACCCTAGGATTTAGGAGTGAGCAACTGTAGGCTATCACA10.7092198581560284No Hit
GAGCACGTGCACACCGGACAGCGCATCCGGCGGCGGCAGCGCGGTGCGCT10.7092198581560284No Hit
GCTGTGTTCACTGGGGTAAAATCTCTTGCCTTTTGCATGTGTTTCCATGC10.7092198581560284No Hit
GAAGTGACCACTCTGCGCCTATAAGAGCGTAGGCGGGTGAGCGTAGTCGC10.7092198581560284No Hit
ATCCGGTAGAGTTCTATTTCCATTTTCAGTATATGAAACATGCTTACAAG10.7092198581560284No Hit
GCTGAGAGGAGAGCTGTGGGGGGAGAGGAGGAAAACAAGCAGAGAGTGGT10.7092198581560284No Hit
ATGTGTGGCAGTATTTCCTTGTCGCTGCCACAATTCATCCCGCCCCCTCC10.7092198581560284No Hit
GTGAACGATAGACAAGGCAAGGTATGAGCGAGAACGAAACACCGCGTACT10.7092198581560284No Hit
GCAACTGCCGCACCTTGCCCGTGAAGTGATCGCCCATGTCCTGCGCGGCC10.7092198581560284No Hit
GGTGGGACGGGCCGTCAAGCTCGTCGTCGATAAGGACGAGGCTAAGCCGA10.7092198581560284No Hit
GCCTCCTAACCCATTACATCGGGGGTAGAGCTCTAACACATGCATTTGGG10.7092198581560284No Hit
CACTCTGTCCCCGCTGTGTTTGGCCCCTAGGTATGTGAAGACCTACCTGT10.7092198581560284No Hit
TGGATAGGAAGAATCAATATTGTGACAATGGCCATACTGCCCAAGGTAAT10.7092198581560284No Hit
GTTGAAACCGATGTCCATGTTGAATCCCCCCCGATTCGAAGTCCAGGCGC10.7092198581560284No Hit
GCTCTACACGGGGCGGGACCGTTTTGTGGCCGAGCCGGGAGTGGCCGGCA10.7092198581560284No Hit
TTTTAACAGCGATCGCGTATTTCGCCTCGCTCAGGCGCAATCACGAATGA10.7092198581560284No Hit
GACACCAAGCTCTGCCAGTCTCTGCACGATCGCCGCGATACGAGGCATCG10.7092198581560284No Hit
CCTTTCAGAAACCTTGCTTTTTTCAAAATCGATTACATTTTATTAAGCGT10.7092198581560284No Hit
CACAAATAGGGCATAACTTGGAAATAGCAATGGACTGTGCAGTGTACCAG10.7092198581560284No Hit
GTTCCTCAGCAACTTCTTCGCCCAGACCGCCGCGTTGGCGTTCGGTAAGA10.7092198581560284No Hit
AGGGATGCGTCCCTGATTATTCACCCAGGTTTCAGAGGTGTCAGACCACG10.7092198581560284No Hit
CTCTTGATCCCCGTCCTAAGAGCGGAGGCTAGGGAGAGAGGGCTCTAAGC10.7092198581560284No Hit
GTCCTCGAACCCGGCTGCCAGCGTGGAATCGTTCGCCATGGCGACCCAGG10.7092198581560284No Hit
GAACCACTGGTTCCGCTGCGACTGTTTACCAAGCGCAATTTCACTCTCGC10.7092198581560284No Hit
GAGGGAGAAGAATGCAGTTTCCCACCACAACAACCCACTCCTCCCAAATC10.7092198581560284No Hit
GCTGAAATTATCCCGTTTCCAACGAATTCCTCACAGAGGTCCAAATATGC10.7092198581560284No Hit
CTCTTGACGCGCTCGGTGAGGGACTGACGCGGCTGTTTCTTGGCGCGCTG10.7092198581560284No Hit
GCTTTCCCTAGCATGTGCAGACACCTGGGTGAATAAATTTGTGCTGTTTG10.7092198581560284No Hit
TTGCCTAACTCTTCATGTTTCTGATATAGAATCTCGTGTTGCCCGTGCTC10.7092198581560284No Hit
GATTCATGTTTTGCCTCCGATCGGAAAGCACGACATGAAACACGTCACTC10.7092198581560284No Hit
TCTACGTCTACCCCACGGTCTCCGACGAGCCGGGCGTGCTCGCCTCGCGC10.7092198581560284No Hit
CGCAAAACTCGGCGGCCGAGCTGGACAGCGAGGCGCAATACGCCAGCAAG10.7092198581560284No Hit
TATATAGATAATGTGTTTGTTTATATACATATATAGGTACTTGGTGGGAA10.7092198581560284No Hit
AGATATTTCCTTTTTCAACATAGGCCTCAAAGCGCTGCAAATGTCCACTT10.7092198581560284No Hit
GCGCGAGGCGGTCGTCTCGGCCTTCCCCCGCACGCCGGACTTCAAGGAGG10.7092198581560284No Hit
CCGGTGCACCGTGGGCGGTGCTGATGGTGGTGATCGCCGAGTTGAGCCCC10.7092198581560284No Hit
TAGAGACACAGTGAGGAGAGAAAGATTCAGGCCAGACACCTATCCTGTGA10.7092198581560284No Hit
GGACTGCGGGCTGCGATGGTCTCCTCCACAGGATCCTCAGCTACAGAGAC10.7092198581560284No Hit
AAGAACTTTCGCCTGCAGGGCTGGCGGCGCGCGTGGTGCTTGCAGATGTC10.7092198581560284No Hit
CCCTCACCAATGCGGACCAGCGTATTGGCAGATCCAATGCGCTCAGCACG10.7092198581560284No Hit
CGCCCACGTAGTTCATTGACGCGGCAATTTTGTGGGCCGAGGCATAAGCC10.7092198581560284No Hit
ACGCACCCACGGGCTATCGAATCGAGAAAACGCTGATGGGGGACGCTTTC10.7092198581560284No Hit
TCCTGGTTCCAATCAGTTGTTTGTGTTTCCATTATGCAAGAGTTTCATCA10.7092198581560284No Hit
GCGGTAGTCGGCGTACATCGTCGGGCTGTGGTTGCCCCACACGAACAGCT10.7092198581560284No Hit
CCAGTAGCGCGTGTAGCCGAGTGCATCCGTGAGTTGCGCCAGGTCGACCG10.7092198581560284No Hit
ATATCATTGTATGATGATCTCACTGGGGCTCAAATTGAACATACTTGGCC10.7092198581560284No Hit
GCACCTGGCCGCGCTCGATCGTCACGCCGTTTTCGATGGCGCGCACCGAG10.7092198581560284No Hit
AAGGACATCGCCGCCAGCTACCAGCGGCGCCGCGACGTGCTGCACAAAGG10.7092198581560284No Hit
GGTAAAATGGCGTAATAGAGCGGCGGGACATTATCGAGAGCTTTTAGCTC10.7092198581560284No Hit
TCATAGAACTGGAATGTGCCTATGGGCCATCTAACCTTATATGCTGTTCA10.7092198581560284No Hit
GCAAAGGCTTCAAGAAAGTGGCAGCCTTGCTGGGTAACGATAGACGGGCA10.7092198581560284No Hit
TCTCAGATTGCTGCAGCCTTCAATACGCATGTTCCTATAGTGCCTGGCCA10.7092198581560284No Hit
ATGTTATTCTCGAGGTTCTTTCGATGGTGCGAGTTGGTTGAGAGGTGAGT10.7092198581560284No Hit
ATGCTATTTCCCCATCAAGTGCCATGGTTCTTCTCTGTTGACTTTTCATA10.7092198581560284No Hit
GCTGGAGTGCAGTGGCGCAATCTCGGCTCACTGCAAGCTCCACCTCCTGG10.7092198581560284No Hit
CTTATGGGTTGCTCAACGGTTTCTACGAGGAATTTTTCTTCCTAGGACTC10.7092198581560284No Hit
GGGTTACTAGCGGACGTCCGCCTTTAACCCGCTCGGTGCCAGCGTCTGCC10.7092198581560284No Hit
ATTCCATTCCATTCCATTCCATTCCATTCCGTTCCATTCCATTCCATTAC10.7092198581560284No Hit
GGTAAAATGGCGTAATAGAGCGGCGGGACATTATCGAGAGCTTTTTGCTC10.7092198581560284No Hit
GGTTGGTTAGGGGCACACTCACCTTCCAGGCTAAAGTATTCCTGCTAACT10.7092198581560284No Hit
CCCAAAATGCTGGGATTACAGGCATGAGCCACCATGCCTGGCTCCCCATC10.7092198581560284No Hit
GTCCAAATCTCAAAAGCATGCTGTTTTGAATCAGGCTTACCAATAATTTT10.7092198581560284No Hit
ACATGATCATTCTCCGGCAGACAATTTGAAGCGATGAGGCCATCCTGCCC10.7092198581560284No Hit
CTTCTGGGCGTCGTTGTGACGCAGGCGAATCAGCATGACCGCATTGCGGT10.7092198581560284No Hit
GGTGATGGCCGACGTCGAATCCATCAACGTGCCATCCCAATCGAAGACGA10.7092198581560284No Hit
GAACCAGGCCACGTTGAACAAGCCGGTTTCCAAGACAGTCACGGTACCTC10.7092198581560284No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)