FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003635371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003635371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAGAGAGGCTGGAGAGAAAGCCACAAACATTAAGAAGTGCCGGTCCT36.666666666666667No Hit
CATTATGTTTCAGCTCTTCTCAGACATATGTCCAAAAACATGCAAAAACT24.444444444444445No Hit
ATGGGGCTCTCTGCCAAGCAAGGCAAAATATTAAACATTTCTAACTGAAT24.444444444444445No Hit
GCTGGGAGCGGCGCGGCTGGAGCGCAGCGCCGAAGGGACTGGCAGGGCTG24.444444444444445No Hit
AGTCAAAGAAGCCCATCTCCTGGGCCATGATGGCGGCGTAGAGCTGGTTG24.444444444444445No Hit
CACGCTGCTTGAGGCTCTGGACTGCATCCTACCACCAACTCGTCCAACTG24.444444444444445No Hit
TGCAGAAGGAGATCACCGCCCTGGCGCCCAGCACCATGAAGATCAAGATC24.444444444444445No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA12.2222222222222223Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAACATGCGCGACCTCGGCGGCAAGCCCGCCCGCGACGGCCGTACCACCC12.2222222222222223No Hit
GTTCTCACTTTGATGGCAGGGCTGCTACGCGTAGTTCCGAAAATTCTATT12.2222222222222223No Hit
GCGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG12.2222222222222223No Hit
GAGCGCCGCGACGCCGGCCGCCACCGCCGAGAAAGGATCGACGTGCGACG12.2222222222222223No Hit
CCTCGATGGTGTCAGGCTTCCTGCGGCGCATATACAACTCATGGTTGCCC12.2222222222222223No Hit
AATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTT12.2222222222222223No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTA12.2222222222222223No Hit
GCTTTCATGATCAGCCGCGAAACTGAGAACGTCAAGTCCAGCGAGGAGAT12.2222222222222223No Hit
TGTCCGGGCCGGGTGAGGTTTCCCGTGTTGGGTCAAATTAAGCCGCAGGC12.2222222222222223No Hit
AAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTAT12.2222222222222223No Hit
GATACTGGCGGGTCGATCCGCGTGCCCGCCGCCTGGAACGACCTGGTGGG12.2222222222222223No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAAG12.2222222222222223Clontech Universal Primer Mix Long (96% over 26bp)
GGATATTGATAAGGTTCAACTTCTTGGGGCCGATGGCCTAGAGCAAGATG12.2222222222222223No Hit
CCTTTAATCAGAGTGATAACTACCACTCTGCCCAAAGTTAAATAAATGAG12.2222222222222223No Hit
GGTCACTGAGACTGCCGTTGGTGGTAGCAGCAGTGGTCTCTGCCCCCTCT12.2222222222222223No Hit
CCCCATAGGCGCCGGCGGCCTCCCCGCGCCATGCCGTGTGCATCGCCGAG12.2222222222222223No Hit
GGCCCTTGCCCTTCTTTGGCAGTGCCCCGCGGCAGGGTCTTGTAGGAGGC12.2222222222222223No Hit
GCTTGAACGGGGGCGAGTCATCGTAAGTGCCGGCTCCTTCGGGGAACCAT12.2222222222222223No Hit
GCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACT12.2222222222222223No Hit
GGAAATGACGCGCATTGCCATGTCTCTGGGATGGCGGCAATCCGTTTCCT12.2222222222222223No Hit
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC12.2222222222222223No Hit
GGTGTGCCTTGTGGTAAGAAGTGGGCTAGGGCATTTTTAATCTTAGAGCG12.2222222222222223No Hit
TTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGA12.2222222222222223No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAT12.2222222222222223Clontech Universal Primer Mix Long (95% over 21bp)
CCCACGTGATCTCCGATGCCAACAAGACGGACTTGGCGATGCCGTAGACA12.2222222222222223No Hit
CTCCAGTTGTGTGTGTCTTTATCTGATACTTGAACTTCTCCTTTAGTCAA12.2222222222222223No Hit
TCTTTATATATGGCTAAATACGAAGAGGTAGCTGGTGCGTTGGCTGTCTC12.2222222222222223No Hit
TACGAGGCCCCTGGCCAAGAGAAAAGGGGTAGGTAGGAAAGAAGAAGGAA12.2222222222222223No Hit
CCCAACGCCTGATCCAGGCGGCGGACGAGAGCGGGACCGAAGCGGTGGGC12.2222222222222223No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)