FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003635372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003635372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAGGGCGAGAAGATCCGAAAGAAATACCCGGACCGGGTGCCGGTGATA36.666666666666667No Hit
ATCCAATACAGGGGCATAGCCGGCGCTTATTTGGCCTGGATGGTTCAGGA24.444444444444445No Hit
GCCCTGACACGTTAATACCAAGGGGAACAGTTAACTTCAATACAAGGTCA24.444444444444445No Hit
ACGACGTCATCCGCGACGGCGGCTTCCACGGCTACACGCTCACCCAGATC24.444444444444445No Hit
GGTTGAGTGCACGTCTTTGTCCCCTCTGGCAGCAGATTCTAGTAGCTGAT24.444444444444445No Hit
GTGTAACACTCTTTATGAAAAACTGGGAACCATTGGTATGTTTCCCTCGA24.444444444444445No Hit
CTAATTAACAGGTGTTCCCATCTCTGGAGCACCCACGCAGTTCTAAGCCC24.444444444444445No Hit
ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA12.2222222222222223Clontech Universal Primer Mix Long (95% over 21bp)
GTACTTTTTTTTTTTTTTTTTTTTTTGCGTTGTTACCACTGCTTCCCATG12.2222222222222223No Hit
CTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGG12.2222222222222223No Hit
TCCCTGGGCCAGCTGCAGTCAGCAAGAGGTGTGGCATGTTTGGGATGCAA12.2222222222222223No Hit
NTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAACTGCGT12.2222222222222223No Hit
GGCCATGTCTGTGTATTTCTGCTTTTCCTTTGGATCAAGAACAGCCCACC12.2222222222222223No Hit
GTCCTAGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCAACAC12.2222222222222223No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTAAAAAAAAAAAAAAAATAAT12.2222222222222223No Hit
ACCCCATCGTGGCAGGCAAGGCGATCTACTGGCAGGCATACGCATACGAG12.2222222222222223No Hit
CTCGCCGCGCTGTCGAGCTTCTACCCCAAGGCCAAGGACATCCACGACCC12.2222222222222223No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT12.2222222222222223No Hit
CCTGCACAGGCGTCCCGACGGCGTCCCCCATCCCCGGATCGTCCCTCGCC12.2222222222222223No Hit
TGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGC12.2222222222222223No Hit
ACCGAGCACCGTCGGCTGAATGAGTGCATGAAGGATTCGATGGTTTCTCA12.2222222222222223No Hit
ACCTGAAGATTGCTCAGGACCTGGAAATGTATGGAATCAACTATTTCGAG12.2222222222222223No Hit
CCTCCTGGTGTACCAATAGGTTATGTTATTCAGACTTGGCACCCATGTCT12.2222222222222223No Hit
GGAGCAGGCGGGGCTGGAGGCGCAGAGCGCGCTGGCCGGCACCGGAGGCG12.2222222222222223No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA12.2222222222222223No Hit
CCACTGCTTGAGTCACCTGATGCCACCCGGGAGTCTATGGTCAAACTCTC12.2222222222222223No Hit
CCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAG12.2222222222222223No Hit
GTGAAGCACCATTTTCTCCTAAGTCTTATTCCAAGCTAAGCAGGCTTAAG12.2222222222222223No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA12.2222222222222223Clontech Universal Primer Mix Long (95% over 21bp)
GCAGGCGCTCGAGGTCAAGCAGAGCCTCGGCGACACGCTCGGCCAGGCGC12.2222222222222223No Hit
CAGGGCGAACAGTTCGGCGCAATCCTCCACCGTGCGGGCGATGGGGCCGA12.2222222222222223No Hit
CCCCGCCTGCCGCTACGATGCTCTGGAGGTCTTCGCTGGGTCTGGGACTT12.2222222222222223No Hit
ATAGTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGA12.2222222222222223No Hit
CCAACGCACCAGCTACCTCTTCGTATTTAGCCATATATAAAGACTGTCTC12.2222222222222223No Hit
GTGCGGATCACGGCGCCGGTCCGGGTGGTACGGCCGTCGCGGGCGGGCTT12.2222222222222223No Hit
CCACCAACCAGATCCGCCTAACCAATGTGGCCGTGGTACGGATGAAGCGC12.2222222222222223No Hit
CGTGACACCAGCTTGTCGGTATGAATGCAACACGCCCGCATTCAATTCAG12.2222222222222223No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)