FastQCFastQC Report
Tue 10 May 2022
EGAF00003635385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003635385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79905
Sequences flagged as poor quality0
Sequence length100
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4950.6194856391965459Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4340.5431449846692948Clontech Universal Primer Mix Long (96% over 26bp)
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGC2660.33289531318440646No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1550.19398035166760527Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC1500.1877229209686503Clontech SMART CDS Primer II A (96% over 26bp)
TGCCTGGACAGTGGCCCTGCAAGCAGCCAAGGATGGGGTCTCCACCACCT1410.17645954571053124No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCC1400.17520805957074026No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAA1250.15643576747387522No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1230.15393279519429323Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTA1190.14892685063512923No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1150.14392090607596522No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1110.1389149615168012Clontech Universal Primer Mix Long (96% over 26bp)
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG1050.1314060446780552No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC980.12264564169951818Clontech Universal Primer Mix Long (96% over 26bp)
CCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGA950.11889118328014517No Hit
GTGCTAGATGGCGCCTGGAAGGGGGGTCACCCAAGTCTCCCTGCTGTCAT910.11388523872098116No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA910.11388523872098116No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG900.11263375258119017Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG900.11263375258119017Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGT870.10887929416181716No Hit
GCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCTG840.10512483574244415No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC810.10137037732307116No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)