FastQCFastQC Report
Tue 10 May 2022
EGAF00003635386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003635386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79905
Sequences flagged as poor quality0
Sequence length100
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4630.5794380827232338Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4410.5519053876478318Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGC2200.2753269507540204No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCC1840.23027344972154432No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1490.18647143482885928Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC1350.16895062887178525Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1300.16269319817283023Clontech Universal Primer Mix Long (96% over 26bp)
TGCCTGGACAGTGGCCCTGCAAGCAGCCAAGGATGGGGTCTCCACCACCT1180.14767536449533822No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1120.1401664476565922Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1080.1351605030974282Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTA1050.1314060446780552No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG1040.1301545585382642No Hit
GTACATGGGAGTCTCGCGCCTGCAGCCCGTGCCGCCCCAGCCGCTGCCGC1030.12890307239847318No Hit
GTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCT980.12264564169951818No Hit
CCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGA980.12264564169951818No Hit
GTACATGGGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGT970.12139415555972718No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG920.11513672486077216No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAA820.10262186346286216No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG810.10137037732307116Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)