FastQCFastQC Report
Tue 10 May 2022
EGAF00003635453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003635453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14467
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATTATCATTCACATTATTTCATAGAAAAAGGAATATAGCAAACGG480.3317895901016105No Hit
CTCCTGCACCTGCGCCGGCTCCTGCAAATGCAAAGAGTGCAAATGCACCT420.2903158913389092No Hit
GTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGA420.2903158913389092No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.2903158913389092No Hit
CTTTACATCTGGGAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCAC390.26957904195755855No Hit
GTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGCTCCT340.23501762632197415No Hit
GATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCCGGCTC330.22810534319485729No Hit
GGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTA320.22119306006774037No Hit
CTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCT320.22119306006774037No Hit
GGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCT280.19354392755927283No Hit
GTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCTGGGAG250.17280707817792215No Hit
CGTGCAACCTGTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCC240.16589479505080526No Hit
GTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCT230.1589825119236884No Hit
GTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTC210.1451579456694546No Hit
CATTATTTCATAGAAAAAGGAATATAGCAAACGGTCACGGTCAGGGTTGT210.1451579456694546No Hit
GAGTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGCTGCCCTGT200.13824566254233775No Hit
GTCACGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTT200.13824566254233775No Hit
TATCAACGCAGAGTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAACC200.13824566254233775No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT190.13133337941522086No Hit
CCATGGATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCC190.13133337941522086No Hit
GAATATAGCAAACGGTCACGGTCAGGGTTGTACATAAAAAATCCAGGTTT190.13133337941522086No Hit
AATGCAAAGAGTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGC180.12442109628810397No Hit
ACATCTGGGAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCACTTGT180.12442109628810397No Hit
GTATCAACGCAGAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCG180.12442109628810397No Hit
GGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTT160.11059653003387018No Hit
GCACTTGTCCGACGCCCCTTTGCAGATGCAGCCCTGGGCACACTTGGCAC150.10368424690675329No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG150.10368424690675329No Hit
ATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCTGGGAGCGGGGCTGTCC150.10368424690675329No Hit
GTGCCAAGTGTGCCCAGGGCTGCATCTGCAAAGGGGCGTCGGACAAGTGC150.10368424690675329No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)