FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003635462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003635462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGATGGGCGCTTGCCAACCGGTTGCACGTAATAGCAATTCTTCAG35.660377358490567No Hit
CACCGCGACCGCAAGGGTTCGCGGCCATCCGACTCTCGTCCCCAGCGTTC23.7735849056603774No Hit
AGAATGTGGCCATGGTGGTGAGCTATCTTGTACCATACAGAAGACCATAC23.7735849056603774No Hit
AAATTAAATATGCATGTGTTTTGGTTTTATGGACTCTAAACTTGATTAAA23.7735849056603774No Hit
CCGGGTCCCTGGGGGCAGCCCTGTCGCCCCACTCGCAACCTCCGCCCCCG23.7735849056603774No Hit
GAGGTCAGGAGTTCGAGACCAGCCTCGCCAACATGGCGAAACCCCATGTC23.7735849056603774No Hit
AACGACGGGTCGATGCTCGCCGCGGCGGCGATGCGGCCCGCGCTGATCGT23.7735849056603774No Hit
GGCATGTGCCACCATGCTTGGCTAATTTTTGTATTCTTTTTAGTAGAGAC23.7735849056603774No Hit
CCACCCTTCAAGCCAGGCGGTGCCGGCTAGCGGCGAGTGAGGAGCGTGGC23.7735849056603774No Hit
TAGAGGACATCAACGTCAAAACGGTAACCGATGTTACGTCTGACATTCTG23.7735849056603774No Hit
AGTCTGGCCCGGCCGTGGCTGGAGGTCCATCTGGCCTGTGAGTCTGTGCC11.8867924528301887No Hit
CCCACTGCAGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACTGCAC11.8867924528301887No Hit
CAGCAGCTCTGCTGGCGGGCGTGCCAGCCCGAGCCGGTGCAGGTGGCCGT11.8867924528301887No Hit
AAACTGAATATAAAATGGCCAGGTGCGGTGGCGCACGCCGGTAATACCAG11.8867924528301887No Hit
AGGCAGGCGGATCACGAGGTCGGGAGTGTGAGACCAGCCTGACCAACATG11.8867924528301887No Hit
GTGGAACACTCGCCTCGGGCGGACACCGCTGTAACCTGCTCACTGTAGGG11.8867924528301887No Hit
CCTTCAGTCAAACAAAGTGAGTCAGATAATTTCTTTATGGCCTTACACAG11.8867924528301887No Hit
GCCTTGGATGATATTCTTGGCATGGAGGTAGTTCATGCCCTGGGCAGTCT11.8867924528301887No Hit
TCACTATAGTGGGAGGGAGAAAGTAAGATGAAGGAGCCAAGGAGAGAAGC11.8867924528301887No Hit
GGTCGGGCAGTGGGGTGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAG11.8867924528301887No Hit
TCACAGTGCTGTGAAGAGGCATCCTTGGAGCTAGCTGAATGGCATCACCA11.8867924528301887No Hit
ATAGTGGAGGGAAGGTCAGCAGATAAACAAGTGAACAAAGGTCTCTGGTT11.8867924528301887No Hit
CCCATGTAGCTGGGACTGCAGGCGCCCTTCACCATGCCCGGCTAATTTTT11.8867924528301887No Hit
GGTTGCCCGGCTTCCCGGAAGCAGCAAAGAGGATGGAAGAGATCGGGGTT11.8867924528301887No Hit
AACTTTGTCTCTACTAAAAATACAAAAATTAGCTGGGCATGGTAGCGGGT11.8867924528301887No Hit
GCGCACACTTGAACCCGTGAGTATCTCTACGAAGGTCAGGCGCCCGACGG11.8867924528301887No Hit
ATCAACATCGTGCCCGACAAGGGCGCTTTCGTCAGCCAGCCGACCGAAGC11.8867924528301887No Hit
ATCCAAATACCAGTGGCATCACCATCATGAGGGAACTAGTTAGACTGTCT11.8867924528301887No Hit
GATGTAGACACAGTAATGACCGGAGTCTGCCATTCCCACGTGCGCAATCA11.8867924528301887No Hit
ACCTGAACCTGGGAGGCGGAGGTTGCAGTGAGCCGAGATCGCACCAATGC11.8867924528301887No Hit
CTTTTGCCTGCTGGAAGTCATTTTCCCAAAGCAATGAACATCTGAGGGGA11.8867924528301887No Hit
GCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCGCGCCCGGCGGAG11.8867924528301887No Hit
GTGTGTGTGTGTGTGTGTATTCCCCCTGGGCTCCTTCTTGGGGAACCTTG11.8867924528301887No Hit
ATACACAGGCGGCGTCATCCCTCTAACGCCGCATGGCTGATTACCCTTCG11.8867924528301887No Hit
CTGTACAGATGAAAAGCACCAAGGTCAAGACTCAGATTTCTTTTGCTTAT11.8867924528301887No Hit
ATGATGAAAAGTCATTTTTGGAGCCACAATGCTTGCCAGCGCACTGGAAG11.8867924528301887No Hit
GTGGAATGGAATGGAATGGAATCAACACGAAAGGAATGGAACGGAATGGA11.8867924528301887No Hit
GGGTCTCCCTCTGTCACCTAGGCTGGAGTTCAGTGGTGCAATCACAGCTC11.8867924528301887No Hit
GAATGGAATGGAATCGAGGGAACCTGAGTGGAATGGAATGGAGTGGAATG11.8867924528301887No Hit
ATTTGAAATTAATTGTGGGATCACAATTTTTATTAATTTATAATTAATTA11.8867924528301887No Hit
AAACTGAATATAAAATGGCCAGGTGCGGTGGGGCACGCCAGTAAAACCCG11.8867924528301887No Hit
GGACCTCCAAAAATTTTCTCTTTCGTTAAAGCAATAAAAACACTGGCAAA11.8867924528301887No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)