FastQCFastQC Report
Tue 10 May 2022
EGAF00003635481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003635481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691927
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGAAGAAGAGATGTTGTCCTGACACTTGTGGCATCAAATGCCTG23550.340353823452474No Hit
GTACATGGGAGAGTCACTCCTGCCTTCACCATGAAGTCCAGCGGCCTCTT22040.3185307120548844No Hit
GATCAGCACCATGAAGCTTCTCACGGGCCTGGTTTTCTGCTCCTTGGTCC21630.3126052314767309No Hit
GAGCAGGACTCCAGAGCCTCCTCCATATGGCAGGAATCAAGCTTTCACAG18970.27416186967700346No Hit
GCTCTGGAAAGTCCTTCAAAGCTGGAGTCTGTCCTCCTAAGAAATCTGCC15340.2216996879728642No Hit
GGACCACACAGAGCAGGACTCCAGAGCCTCCTCCATATGGCAGGAATCAA14840.21447349214584774No Hit
CTCCTGCCTTCACCATGAAGTCCAGCGGCCTCTTCCCCTTCCTGGTGCTG14250.20594658106996835No Hit
TCCTGGTGCTGCTTGCCCTGGGAACTCTGGCACCTTGGGCTGTGGAAGGC13640.1971306221610083No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG13440.19424014383020174No Hit
CTACAGCACAGATCAGCACCATGAAGCTTCTCACGGGCCTGGTTTTCTGC13250.1914941894159355No Hit
GCCTTAGATACAAGAAACCTGAGTGCCAGAGTGACTGGCAGTGTCCAGGG12220.17660822601228165No Hit
CTCCAGAGCCTCCTCCATATGGCAGGAATCAAGCTTTCACAGGGGAAACG12160.1757410825130397No Hit
GTGCCAAGCCTTTCCCCAAAGGAGGATATCAGTGGTGGAGCCAAGTCTCA11920.1722725085160718No Hit
GACCTGGACCACACAGAGCAGGACTCCAGAGCCTCCTCCATATGGCAGGA11640.16822583885294257No Hit
CTCTGGCACCTTGGGCTGTGGAAGGCTCTGGAAAGTCCTTCAAAGCTGGA11130.16085511910938582No Hit
ACCTTGGGCTGTGGAAGGCTCTGGAAAGTCCTTCAAAGCTGGAGTCTGTC10930.15796464077857925No Hit
ACACAGAGCAGGACTCCAGAGCCTCCTCCATATGGCAGGAATCAAGCTTT10140.14654725137189328No Hit
CCCCAAAGGAGGATATCAGTGGTGGAGCCAAGTCTCAGGGTGGAAAGGAC9930.14351224912454638No Hit
GTACATGGGAGGGACCCGCAGCTCAGCTACAGCACAGATCAGCACCATGA9900.1430786773749254No Hit
CCTCCATATGGCAGGAATCAAGCTTTCACAGGGGAAACGCAGGATTTCCC9890.14293415345838506No Hit
CCTAAGAAATCTGCCCAGTGCCTTAGATACAAGAAACCTGAGTGCCAGAG9700.1401881990441188No Hit
TCTTGAAAGCCTGCTGTGTGCCAAGCCTTTCCCCAAAGGAGGATATCAGT9640.13932105554487684No Hit
CCATATGGCAGGAATCAAGCTTTCACAGGGGAAACGCAGGATTTCCCACA9630.13917653162833651No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG9520.1375867685463929No Hit
AGCCTGCTGTGTGCCAAGCCTTTCCCCAAAGGAGGATATCAGTGGTGGAG9040.13064962055245713No Hit
CGCTTGCACTGGCCATCCATCTCACAGAAATTGGGGGGGTTAAGCATCAA8970.1296379531366748No Hit
GTGTACCCTCTCCCCGCTTTGTATCCCTGCCCTGAGGGCCTCACAGCCAG8840.12775914222165055No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTTACCAC8780.12689199872240858No Hit
GAAATAGGCTCGTTTATTTATTCATTGATCAACTGGCACTTCTTGAAAGC8670.12530223564046497No Hit
GTGCCAGAGTGACTGGCAGTGTCCAGGGAAGAAGAGATGTTGTCCTGACA8600.12429056822468267No Hit
TATCAGTGGTGGAGCCAAGTCTCAGGGTGGAAAGGACCTGGACCACACAG8580.124001520391602No Hit
GTCCTGACACTTGTGGCATCAAATGCCTGGATCCTGTTGACACCCCAAAC8410.12154461381041642No Hit
GAGAAATAGGCTCGTTTATTTATTCATTGATCAACTGGCACTTCTTGAAA8070.11663080064804525No Hit
GGATATCAGTGGTGGAGCCAAGTCTCAGGGTGGAAAGGACCTGGACCACA7970.11518556148264196No Hit
GTGGAAGGCTCTGGAAAGTCCTTCAAAGCTGGAGTCTGTCCTCCTAAGAA7880.11388484623377901No Hit
CTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTG7710.11142793965259341No Hit
CGTTTATTTATTCATTGATCAACTGGCACTTCTTGAAAGCCTGCTGTGTG7030.10160031332785105No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)