FastQCFastQC Report
Wed 11 May 2022
EGAF00003635525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003635525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3741866
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCAGACATCAAATCCTGCAGACAAGGGGAGCCCTCAGTCTGCAGGGC100500.2685825735074426No Hit
CAGTATAGATTCTCTGGCACTCTGAGGTCTCTGTGGCAGGCCTGGTCAGG93460.24976843104483165No Hit
GACAGGATCCTCCCTGTCCCATGGCCTAAATGCCTCTCAGAACTCACAGA76250.20377533562131833No Hit
GTACATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGG69080.18461377291436948No Hit
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGTTTGTCCCTGCGGT65750.17571446973248106No Hit
CTCCTAGCCTTGGCCTTTGGCATGAGATGGAGGCCTCCTTATTCCCCATC65250.1743782380234888No Hit
GGACGCTCTCTGGCCTTCGGCCTCCATCCTGAGGCTCCACTTGGTCTGTG61570.16454357264530586No Hit
ATCTCCACTGTGTCCTTAGCTCCACACAGTCAGGGCTTCCCTGAAATCTG58500.1563391099520934No Hit
ATTGGGACTGGAGGCACCCTTAGCCACTTCATTCCTCTGATGGGCCCTGA55950.14952432823623293No Hit
GGTTCTTGGCAAATGATAGGTGCTCAGTCAATAGCCTCTGTGTATAGTAA53870.14396560432682518No Hit
GCTCCACACAGTCAGGGCTTCCCTGAAATCTGCCTCTCTCTGTCATTATG52710.14086554676196317No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG49390.1319929682142546No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT48710.13017569309002514No Hit
TCTATACTGTGTCCTTAGCTCCACACAGTCAGGGCTTCCCTGAAATCTGC47200.12614027332886854No Hit
GTGCAGAGCTAAGGACACAGTGGAGATTCTCTGGCACTCTGAGGTCTCTG45670.12205140429935224No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA42810.11440815892391656No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC42280.11299175331238479No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG40860.10919685525884679No Hit
TTTCTATTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT40760.10892960891704832No Hit
GTACATGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGG39800.10636404403578323No Hit
TCCTAAGACCTGGCACATGTTAATTTTAATGATTATCTGGGTGAATGTAT38860.10385192842287778No Hit
GCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGA38800.10369158061779872No Hit
GTCACAGGGTTGGCTTGGGCACCACCGTGCTGGGGCACCACTGGGCCCTC38590.10313036330002197No Hit
GGACGCTCTCTGGCCTTCAGTTTCCATCCCCAGGATCCACTTGGTCTGTG38580.10310363866584212No Hit
CTTCAGTTTCCATCCCCAGGATCCACTTGGTCTGTGAGATGCTAGAACTC38070.10174068232267003No Hit
GCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGG37680.10069842158965607No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)