FastQCFastQC Report
Wed 11 May 2022
EGAF00003635526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003635526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3741866
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTATAGATTCTCTGGCACTCTGAGGTCTCTGTGGCAGGCCTGGTCAGG83510.22317742003588587No Hit
ACTTCAGACATCAAATCCTGCAGACAAGGGGAGCCCTCAGTCTGCAGGGC81440.2176454207606579No Hit
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGTTTGTCCCTGCGGT71170.1901992214579571No Hit
GTACATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGG67030.17913522290750125No Hit
GACAGGATCCTCCCTGTCCCATGGCCTAAATGCCTCTCAGAACTCACAGA66500.17771881729596944No Hit
CTCCTAGCCTTGGCCTTTGGCATGAGATGGAGGCCTCCTTATTCCCCATC60070.1605348775183291No Hit
ATTGGGACTGGAGGCACCCTTAGCCACTTCATTCCTCTGATGGGCCCTGA53970.14423285066862362No Hit
GGACGCTCTCTGGCCTTCGGCCTCCATCCTGAGGCTCCACTTGGTCTGTG52540.14041122798090577No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG49300.131752446506636No Hit
GCTCCACACAGTCAGGGCTTCCCTGAAATCTGCCTCTCTCTGTCATTATG49130.1312981277255786No Hit
TTTCTATTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT46130.12328073747162512No Hit
GGTTCTTGGCAAATGATAGGTGCTCAGTCAATAGCCTCTGTGTATAGTAA46070.12312038966654605No Hit
ATCTCCACTGTGTCCTTAGCTCCACACAGTCAGGGCTTCCCTGAAATCTG45570.1217841579575538No Hit
GTGCAGAGCTAAGGACACAGTGGAGATTCTCTGGCACTCTGAGGTCTCTG44220.11817633234327471No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT43740.11689354990264215No Hit
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGTTCACGGCCATGCCCAG42170.11269778233640648No Hit
GTACATGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGG41760.11160207233503283No Hit
GTCACAGGGTTGGCTTGGGCACCACCGTGCTGGGGCACCACTGGGCCCTC39960.10679163818266074No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA39050.10435969647229484No Hit
TCTATACTGTGTCCTTAGCTCCACACAGTCAGGGCTTCCCTGAAATCTGC38430.10270276915314445No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGTGCCACCTCTG38050.10168723305431034No Hit
GGACGCTCTCTGGCCTTCAGTTTCCATCCCCAGGATCCACTTGGTCTGTG37440.10005703036933977No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC37430.10003030573515995No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)