FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003635619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003635619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCATATTTGCTACTAAAAAACGTTCATATGGTTTGATACCACATTTCA32.6785714285714284No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG32.6785714285714284Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG32.6785714285714284Clontech Universal Primer Mix Long (96% over 26bp)
TCCTAGAGCAGGGAGAGGCCACCCTCTCAGTGGCAAGAAGAGCCCTGGCT32.6785714285714284No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACC21.7857142857142856Clontech Universal Primer Mix Long (96% over 26bp)
ATCTTATTCATACCCTAATTGAAGAGGCCCGACAATTAACAGCAGAAACA21.7857142857142856No Hit
ATATAGTTGTCATAGCCTAGGGTAAGGGTGCTATAACAGTAGCCAGACTT21.7857142857142856No Hit
GCCTATACAAGAATCACATCTAGAAATGCTAGTTTAAGAAGCTCTCTATG21.7857142857142856No Hit
GTGCAGCATAGCTTGGGGCCGCCGTGGCCTGTGCCCTCTCTCTCTGGGGC21.7857142857142856No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG21.7857142857142856Clontech Universal Primer Mix Long (96% over 26bp)
TTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGC21.7857142857142856No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG21.7857142857142856Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT21.7857142857142856Clontech Universal Primer Mix Long (96% over 26bp)
CTAAAAAAAGAGTGCATAAAAGAGTGTTGTCTAAGTTGGCACCAGAGTTG21.7857142857142856No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA21.7857142857142856Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG21.7857142857142856Clontech Universal Primer Mix Long (96% over 26bp)
ACCTAGGAAACAGAAACATTTAGGAACTAGAATACAGCAGATTATTACAA21.7857142857142856No Hit
GAAAAGTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCT21.7857142857142856No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAAACGCAGAGTACTTTT21.7857142857142856No Hit
CGGCAGGAGAAGATCTGGCCCACGTCGCCGCCGTCGCCGTAGGGGCTCAG21.7857142857142856No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA21.7857142857142856Clontech Universal Primer Mix Long (96% over 26bp)
TTGCACTGGTGGCGCTGGTTGCAGGGATATTGTGGACTGTTTCGTTAGGC21.7857142857142856No Hit
GGTTAGTGATAGACAACCAATCTCCTAAAGAGCTATGGGCAGGGTTACGT21.7857142857142856No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT10.8928571428571428No Hit
CTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA10.8928571428571428No Hit
GTTTGCTTGCTTGCTTGTTTGTTTTTTGGCCCTTCAACCTGCTCTACTGT10.8928571428571428No Hit
GTCGCGGATCTGGTCGCGCACGAGCTGCGGCACGTACGGCACCGCGACGT10.8928571428571428No Hit
TACTACCAGTATCTCCATCCTTCCCTTCAAAAGGTGGGTTAAGGATTTGT10.8928571428571428No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT10.8928571428571428Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGTAAATCTCAAAGTGCTCTGAAATCATCCCTCTAAATTCTGTCCTGTG10.8928571428571428No Hit
ATGTAAAAATAAGAATGAATGTGGCGCCCCCTTCTTCCAGATGTAATAGA10.8928571428571428No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTCTTATACACA10.8928571428571428Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGTTAGCCAGGATAGTCTCCATCTCCTGACCTCGTGATCCGCCCGCCTC10.8928571428571428No Hit
CTCAAACCTTGGTACCATTGTTCCACCCACATGACTATTCCCCAGACTTC10.8928571428571428No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.8928571428571428No Hit
TTGCACCCGTCTGCGGGTCGCATCGTCCAGATTTGCATGAGCCTGAACGG10.8928571428571428No Hit
CCCATCAACAGTGTAAATGCATTCCTATTTCTCCATTGTGTCGCCAGTCT10.8928571428571428No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG10.8928571428571428Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCATTGCCAGAGGCAGGGCCCCACTGCTCACCAAGACACACACAAAG10.8928571428571428No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA10.8928571428571428Clontech Universal Primer Mix Long (96% over 26bp)
ATANCACTGCTTCCCATGTACTCTGCGTTTGATACCACTGCTTCCCCTGT10.8928571428571428No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTAT10.8928571428571428Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCGCTCACTGCAACCTCCACCTCCCAAGCTCAAGCGATCCTCTCGCCTCA10.8928571428571428No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAACAA10.8928571428571428No Hit
CACTTACAAAGCTTAATTTAGCTGGATATGACATTCTGGGTTGAAAATTC10.8928571428571428No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAGCAGCGTACAAG10.8928571428571428No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10.8928571428571428Clontech Universal Primer Mix Long (96% over 26bp)
CCCGATGACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTT10.8928571428571428No Hit
TATTTTGTACAGGTATGTTTTTCATTCCCATTCAGAGTTGGGCCATTTGT10.8928571428571428No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAACAATGCTTACCC10.8928571428571428No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCT10.8928571428571428Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAG10.8928571428571428Clontech Universal Primer Mix Long (95% over 22bp)
GGGGTACTGCCCTCAGGAGAACGCGCTGTGGCCTAACCTGACAGTGAGGC10.8928571428571428No Hit
GTTCTATCTATTGCAGTGATATATTGGGTCCTGTATAACTGTAAGGCATT10.8928571428571428No Hit
GTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACT10.8928571428571428No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAACAAAGGG10.8928571428571428No Hit
CAATATTATTGTGTGTGAGTCTAAGTCTCTTTGTAGGTCTCTAAGAACTT10.8928571428571428No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCCGAGGGGTTTTTTTTTTTTTT10.8928571428571428Clontech SMARTer II A Oligonucleotide (95% over 22bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA10.8928571428571428Clontech Universal Primer Mix Long (95% over 22bp)
GCCCAACCAACCAAGCAGGCCTTTCTCTTCCCCTGCGGACACCTCCTGCA10.8928571428571428No Hit
CATTATTACTTTGCCATGTAATAGCCATTTATATCAATTTCACATTCTAC10.8928571428571428No Hit
GTGGTGCTGCGCGCGCCCGGCATGCTGGCGCTGCACCTGGCCTTCGACCG10.8928571428571428No Hit
GGTATCAACGCAGAGTACATGTACTCTGCGTTGATACCACTGCTTCCATG10.8928571428571428Clontech SMART CDS Primer II A (96% over 26bp)
GTTCTGCTTCACGGCGTAGGTTCCGACCAGTTCGGCCTCGGGCGCGCCGC10.8928571428571428No Hit
AGAGAAAACTATTGGCATGCCTTCTACTGTGACTTTTAACGCCAAAGTGA10.8928571428571428No Hit
CGGGGAATGAGTTCCTATTAAAGACCTGTGTTTGGCCGGGGCAGGTTTAC10.8928571428571428No Hit
GGGTAAATCTCAAAGTGCTCTGAAAAAATACCAATAAAATATTTGCTGGT10.8928571428571428No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAACAACG10.8928571428571428Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCAGCGTTGATAACACTGCATAAATTTAAAAAGCGGT10.8928571428571428No Hit
GGTCTCTCCTTCACAGGCTCTTTTTTGACCCGCTCCTTCACACTAGTCAG10.8928571428571428No Hit
CCCCTATGTTAGACTCAACTGACCCGAAAGTGCTGGGATTACAGGCATGA10.8928571428571428No Hit
AACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTG10.8928571428571428No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTAAATCGGAAGCAGAGGTATCAA10.8928571428571428Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTACATGGGAAGCAGTGGTATCAACGGCTTCCCATGTACTCTGCGTTGAT10.8928571428571428No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACTTTTATACCAAT10.8928571428571428No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTTAAAAACATCGTA10.8928571428571428No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.8928571428571428Clontech Universal Primer Mix Long (96% over 26bp)
TAACAACAATCTCATAATTGAGAATTGTTGAGAAATGTGAGGGATTGCAG10.8928571428571428No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGCTGT10.8928571428571428Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAA10.8928571428571428No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT10.8928571428571428Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.8928571428571428Clontech Universal Primer Mix Long (95% over 21bp)
ATTATAAGGAATATTATGTGGTTGCTATTTAATCCCCTGGGGGTACACCA10.8928571428571428No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC10.8928571428571428Clontech Universal Primer Mix Long (96% over 25bp)
GGCCGGGCTGCTCTTGGGCTCCTGACCTTGAGATCCGCCCGCCTCGGCCT10.8928571428571428No Hit

[FAIL]Adapter Content

Adapter graph