FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003635620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003635620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA76.25Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCCACACACACCACCTTATCTGGTCGTGTGACTGGAGCCAGGAAGGTT32.6785714285714284No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA32.6785714285714284Clontech Universal Primer Mix Long (96% over 26bp)
GTTGAGTGATTTTCAGACTTGTTCTAGTGGCAGAGCATTTGGAAGTTGCA32.6785714285714284No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA32.6785714285714284Clontech Universal Primer Mix Long (95% over 21bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTCTGCGGGTGC21.7857142857142856Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCATTGATAGGCACTTAGGTTGATTCCATGTTTTTGCTATTCTGAACAG21.7857142857142856No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCC21.7857142857142856Clontech Universal Primer Mix Long (96% over 27bp)
CTTTAGTATCTCAAAAAATTGTTGCCAAGACCTCTGCTCTTGACTGGCCC21.7857142857142856No Hit
GGCTAGTAATAGGATTCAGAATTGCTTCCCTTTTATATCGACCATGTAAG21.7857142857142856No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG21.7857142857142856Clontech Universal Primer Mix Long (96% over 26bp)
CCCGCGGGCCGTCCCCGGCCGGCCGCCCCCGGCCCCAGCGCCGCTGACCC21.7857142857142856No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG21.7857142857142856Clontech Universal Primer Mix Long (96% over 26bp)
TTGGTGGCCTGTTCCCATATTTTTCTCCAGTGCAGGGTTGGGTCTACTTA21.7857142857142856No Hit
GAGGCTACCCACTCCACATTACCTTCTTTTCAAAGGCCTGTTTCCCTTGC21.7857142857142856No Hit
TTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAGGTGA21.7857142857142856No Hit
GCTGGGTTCTTCTTTAGCAATGACAAACCCCATTTTTTGCCAAAAGGCAC21.7857142857142856No Hit
ATGCAGGATCCACGTTTTGTCGTCTTCAATGGTGGCACTGCGTGCATAAA21.7857142857142856No Hit
CTCTAAAACAGGAGTGTGCTACCATTAGCTATGAAAATAATTTTAAAGGC21.7857142857142856No Hit
GCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGT21.7857142857142856No Hit
GGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACAT21.7857142857142856Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG10.8928571428571428Clontech Universal Primer Mix Long (96% over 26bp)
GCTGGAGTACAGTGGCACAATCATAGCTCACTGCAGCCTCCAACTCCTGG10.8928571428571428No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT10.8928571428571428Clontech SMART CDS Primer II A (100% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTT10.8928571428571428Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT10.8928571428571428Clontech Universal Primer Mix Long (96% over 26bp)
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10.8928571428571428No Hit
CAGCAGCGCGTAGCCGTCGGCGAACTGCGTCTGCGCCTCGACGCCGTCGG10.8928571428571428No Hit
CCTGTAATCCCACCACTTTGGGATCACTTGAGGCCGGGACTTTGAGACCA10.8928571428571428No Hit
GATAAAAGGCAAGAAAAATAATCATCAGATTCTCCAAGGTTGAAATGAAA10.8928571428571428No Hit
TAATAATTCAAGTTAAACTAATGAAACTGTCTGTAACGGACTCTAATAAA10.8928571428571428No Hit
GGGCGAAGGATCTGAACAGACACTTCCTAAAGAAGACATTCATGCAGCCA10.8928571428571428No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG10.8928571428571428Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA10.8928571428571428Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCATGGGAAGCAGTGGT10.8928571428571428Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10.8928571428571428Clontech Universal Primer Mix Long (96% over 26bp)
GGCCAGCCAAGGCCATCAGCATGTCTCGGACTGACCCGCCGGCTGTCAAC10.8928571428571428No Hit
ATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGTATGTG10.8928571428571428No Hit
GTATATGACACTAATAGGACAGACACAGGATATTGCATAAATATTTTTAA10.8928571428571428No Hit
CCCTTGGGTATAGAACAGATGTGGCTCCTCTGTGTACCGTGTGATGGCAA10.8928571428571428No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10.8928571428571428Clontech Universal Primer Mix Long (96% over 26bp)
AGGGAAGCACAGGGCTCAACGCACAAAACATGGTAACAAATCGTAACAAC10.8928571428571428No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCACAATAAAATAGAAGTAAAAA10.8928571428571428Clontech Universal Primer Mix Long (95% over 22bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA10.8928571428571428Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTGCTTCCCATGTACTCTGC10.8928571428571428Clontech SMART CDS Primer II A (96% over 26bp)
GTCCTCATTGAGCAGGGGATTCGTGGTCGCGAGATCGAGTGCTCTGTCAT10.8928571428571428No Hit
AAAAAAAAAGTACTCTGCGTTGATACCACTGCTTCCCTGTACTCTGCGTT10.8928571428571428Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA10.8928571428571428Clontech Universal Primer Mix Long (95% over 21bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10.8928571428571428Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT10.8928571428571428Clontech Universal Primer Mix Long (96% over 26bp)
GTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAGTAAAAACCCGCC10.8928571428571428No Hit
CAATTATAATGACAGCTTTTCTCTCCTCACTCCACAACAGGTAAATATAT10.8928571428571428No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCTGTCTCT10.8928571428571428Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC10.8928571428571428Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGGGAACAGAGTTGACGGAGAGGGGACAGGAGGTGACTAGATTTGGAA10.8928571428571428No Hit
GGATGGTCTCGATCTCCTGACCTCATGATCCACCCGCCTCGGCCTCCCAA10.8928571428571428No Hit
CGATTACTGTTCTATCTAATACATTTATGAAGATTAATGAAAAGTTTGCA10.8928571428571428No Hit
GAAGGGAAGGAGGAAGACTTTCTTGCTCTCCAATTTGTATTTGGAGGGAA10.8928571428571428No Hit
GTCCTGCATGCGGCATGCAGGGCGGTGCGCGCTCCTTCGACAAGGGATCA10.8928571428571428No Hit
ACCCCTGCTTTCATTATTGTCCTCCTGCCATCCCCTCTTGAGAGATCAAC10.8928571428571428No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACA10.8928571428571428Clontech SMART CDS Primer II A (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTAAAAACAA10.8928571428571428No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTC10.8928571428571428No Hit
NGGAAGCAGTGGTATCAAACGCNGAGTACATGGGAAGCAGTGGTATCTGT10.8928571428571428No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.8928571428571428Clontech Universal Primer Mix Long (96% over 26bp)
TTGCAATACTCAGTAAGCATTGGATTTTTTCAGGGACCACAAGAGAAACC10.8928571428571428No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGAGCTTCCCATGTACACTGC10.8928571428571428Clontech Universal Primer Mix Long (95% over 22bp)
CACGGAAGCCGTGCAGGCGAGGTCGGCGCGCGCATCGGGCGCACCGGTCG10.8928571428571428No Hit
CGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTG10.8928571428571428No Hit
AGCCTAAAGAGATGACTCAGCCTAAGGTGGCAGGCACCATGCAGGCATTG10.8928571428571428No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA10.8928571428571428Clontech Universal Primer Mix Long (96% over 26bp)
CGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAATCTGCGTTTTAAA10.8928571428571428No Hit
TTCCAGGAAGACTAAGTTAATTTAGCTATTCACAGAGTGGCACATTTGAC10.8928571428571428No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAGGGTTTAAAAC10.8928571428571428No Hit
CACTAAGAGCTCATCCTTCAGTCATGTGTGCACATGTACAAGTGCGAGAA10.8928571428571428No Hit
GCCCAGCTAATTTTTGTATTTTTAGCACAGGGTTTCACCATGTTGGCCAG10.8928571428571428No Hit
GTTGTAGGTCGGGGGCACCACACCACGCTCGCTTGGCAGTAACGCGAGCA10.8928571428571428No Hit
CTTCACAAGGCTAAAACAATTGGGAAAACCCTTCAGAAACGCTAGCAATT10.8928571428571428No Hit
CTAGTACCATAACTTTCTATGTCCTTGGCACCTAATAGGTGGTATAGTCT10.8928571428571428No Hit
GTCTAATGGCATACATGATGGTTTGCCAGATTTGTAGCCTTTGTGAGGCT10.8928571428571428No Hit
AAAAAGTACTCTGCGGTTGATACCACGCAGAGTACATGGGAAGCAGTGGT10.8928571428571428No Hit
CGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAATAGGCGTTTGAAA10.8928571428571428No Hit

[FAIL]Adapter Content

Adapter graph