FastQCFastQC Report
Tue 10 May 2022
EGAF00003635754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003635754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences940914
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG23430.24901319355435247No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA22100.23487800160269695No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA21690.23052053641459264Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20670.2196800132636989Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAAT19690.2092646086677422No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC16660.17706187813126387No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG16640.17684931885379535No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC16280.1730232518593623No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG16090.1710039387234115No Hit
GGGTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAAT15850.16845322739378943No Hit
CACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCAC14580.15495571327453944No Hit
GGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCAT14380.1528301204998544No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTACTTGTCCA14270.15166104447377762No Hit
GTATATAGGCTGAGCAAGGGGTGGTGAGGTTGATCGGGGTTTATCGATTA13920.14794125711807882No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG13890.14762241820187605No Hit
CTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTG13720.14581566434339377No Hit
GTCCCAATGTATGGGATGGCGGATAGTAAGTTTGTAATTACTGTGGCCCC13400.1424147159038977No Hit
CCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTC12980.13795097107705911No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT12970.13784469143832487No Hit
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCC12960.13773841179959062No Hit
CCCCACCCCATCCAACATCTCCGCATGATGAAACTTCGGCTCACTCCTTG12860.1366756154122481No Hit
GGACTATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTC12820.13625049685731108No Hit
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC12600.13391234480515754No Hit
GAATATGATAGTGAAATGGATTTTGGCGTAGGTTTGGTCTAGGGTGTAGC12570.1335935058889548No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC12460.132424429862878No Hit
TATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATATCATAG12300.13072395564313No Hit
CTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCC11700.12434717731907485No Hit
CATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACA11550.12275298273806108No Hit
GGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTG11470.12190274562818706No Hit
GTAATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGACGGATCGGAGAATT11400.1211587881570473No Hit
GTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCCGAGGGCGTCT11300.12009599176970479No Hit
TCCCCACCCCATCCAACATCTCCGCATGATGAAACTTCGGCTCACTCCTT10890.11573852658160043No Hit
GGGTAAATACGGGTCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG10860.1154196876653977No Hit
GTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAATGT10850.11531340802666344No Hit
GTTTAAGGGGTTGGCTAGGGTATAATTGTCTGGGTCGCCTAGGAGGTCTG10590.11255013741957287No Hit
GGTGAGAATAGTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCC10280.10925546861881108No Hit
GTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGCCACCTACGGTGA10260.10904290934134256No Hit
GTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGC10240.10883035006387407No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA9990.10617335909551776No Hit
GCCTACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCG9980.10606707945678351No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC9980.10606707945678351No Hit
GTATGGGATGGCGGATAGTAAGTTTGTAATTACTGTGGCCCCTCAGAATG9960.105854520179315No Hit
GTCTACTGAGTAGCCTCCTCAGATTCATTGAACTAGGTCTGTCCCAATGT9860.10479172379197249No Hit
CCTATTGATAGGACATAGTGGAAGTGAGCTACAACGTAGTACGTGTCGTG9860.10479172379197249No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT9810.10426032559830124No Hit
GTGTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTT9810.10426032559830124No Hit
GCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAATCGCCCA9780.10394148668209847No Hit
GTAAATATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATAT9750.10362264776589572No Hit
ATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCC9650.10255985137855318No Hit
ACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCACAGCAGT9630.10234729210108469No Hit
ACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGC9590.10192217354614767No Hit
CCACAGGACTATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCC9580.10181589390741344No Hit
CTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAAT9540.10139077535247641No Hit
ATCCCATACATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTC9420.10011541968766541No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)