FastQCFastQC Report
Tue 10 May 2022
EGAF00003635819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003635819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246799
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGC6320.2560788333826312No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4830.19570581728451086No Hit
CTTATACACATCTCCGAGCCCACGAGACGACCCACTGCTCATCTCGTATG3840.15559220256159872No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT2910.11790971600371153No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2760.11183189559114906Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGAACAGGTTGAACAGTCTACCCTCCCTTAGCAGGGAACTACTCCCACCC2600.10534888715108247No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2510.101702194903545No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)