FastQCFastQC Report
Tue 10 May 2022
EGAF00003635903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003635903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences594515
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT20260.3407819819516749No Hit
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCC17070.28712479920607553No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC15270.25684801897344894No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT15080.2536521366155606No Hit
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC14330.24103681151863282No Hit
CTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCC13920.23414043379897898No Hit
GGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTG13550.22791687341782796No Hit
GAATATGATAGTGAAATGGATTTTGGCGTAGGTTTGGTCTAGGGTGTAGC13480.22673944307544805No Hit
GTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGC13230.22253433470980546No Hit
CACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCAC12370.20806876193199497No Hit
ATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCC12280.20655492292036365No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA12070.20302263189322386No Hit
GTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGCCACCTACGGTGA11570.19461241516193872No Hit
GTACATGGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAAT11020.18536117675752503No Hit
GTCCTAATCACAGCAGTCCTACTTCTCCTATCTCTCCCAGTCCTAGCTGC10830.18216529439963666No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT10830.18216529439963666No Hit
TATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATATCATAG10560.1776237773647427No Hit
ACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCACAGCAGT10500.17661455135698848No Hit
CCTATTGATAGGACATAGTGGAAGTGAGCTACAACGTAGTACGTGTCGTG10470.17610993835311137No Hit
CTCCTACTCCTGCTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGG10370.17442789500685432No Hit
ATATAAAACCCCCTGCCATAACCCAATACCAAACGCCCCTCTTCGTCTGA10260.17257764732597158No Hit
CTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTTCGAAG10230.17207303432209448No Hit
GTCTACCCTCCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGA10170.17106380831434026No Hit
TAGTGGAAGTGAGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAG9880.16618588261019485No Hit
CCTTACACCTAGCAGGTGTCTCCTCTATCTTAGGGGCCATCAATTTCATC9670.1626535915830551No Hit
GGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAATGAAGCCTCCT9630.1619807742445523No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG9570.16097154823679807No Hit
GCCTACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCG9550.16063513956754666No Hit
TCACTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCC9420.1584484832174125No Hit
GTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTC9220.15508439652489847No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC9110.1532341488440157No Hit
GGGTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAAT9050.1522249228362615No Hit
GTAAATATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATAT8980.15104749249388155No Hit
GACCATACCTATGTATCCAAATGGTTCTTTTTTTCCGGAGTAGTAAGTTA8870.1491972448129988No Hit
GTCCTAGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCAACAC8640.14532854511660767No Hit
ACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGC8610.14482393211273056No Hit
CCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCACCT8570.14415111477422773No Hit
GGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGAGCTACAACGT8520.1433100931010992No Hit
GTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTGTTGA8510.14314188876647352No Hit
GAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATC8350.14045061941246226No Hit
GGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTGAGCAC8170.1374229413891996No Hit
CCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCAT8160.13725473705457392No Hit
ATTCCGGATAGGCCGAGAAAGTGTTGTGGGAAGAAAGTTAGATTTACGCC8140.1369183283853225No Hit
CATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCCGAGAAAG8120.1365819197160711No Hit
GTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTT8070.13574089804294256No Hit
GTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTAT7990.13439526336593693No Hit
GCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCT7920.13321783302355703No Hit
TAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTA7840.13187219834655137No Hit
CATAGGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCG7780.13086297233879718No Hit
GTAATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGACGGATCGGAGAATT7650.12867631598866303No Hit
GTTTATATTCTTATCCTACCAGGCTTCGGAATAATCTCCCATATTGTAAC7570.1273306813116574No Hit
GCCATAACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCAC7510.12632145530390318No Hit
ACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAG7510.12632145530390318No Hit
CTCCACGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCTAGG7460.12548043363077466No Hit
GACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTAT7420.12480761629227186No Hit
GTACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATC7350.12363018594989193No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7330.12329377728064052Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCACTAGACATCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCA7310.12295736861138913No Hit
TCCCCACCCCATCCAACATCTCCGCATGATGAAACTTCGGCTCACTCCTT7160.12043430359200356No Hit
TTCATGTGGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCG7130.11992969058812646No Hit
TCCGTGGAGTGTGGCGAGTCAGCTAAATACTTTGACGCCGGTGGGGATAG7040.11841585157649512No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTACTTGTCCA6940.1167338082302381No Hit
GATTATGGTAGCGGAGGTGAAATATGCTCGTGTGTCTACGTCTATTCCTA6870.11555637788785818No Hit
GGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCAT6870.11555637788785818No Hit
GTCCTACTTCTCCTATCTCTCCCAGTCCTAGCTGCTGGCATCACTATACT6830.11488356054935535No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA6780.11404253887622685No Hit
GAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTGAGCACACCAT6710.11286510853384692No Hit
TCCTAGGGTTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGAC6690.1125286998645955No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCCGTAAGTAGGAGAGTGATAT6670.1121922911953441No Hit
GGTGGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTAC6610.11118306518758989No Hit
GGTGTAGCCTGAGAATAGGGGAAATCAGTGAATGAAGCCTCCTATGATGG6590.11084665651833847No Hit
GGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTTCGAAGCGAAG6500.10933281750670715No Hit
CATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACA6500.10933281750670715No Hit
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT6480.10899640883745573No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT6410.10781897849507582No Hit
GGACTATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTC6410.10781897849507582No Hit
GTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCCGAGGGCGTCT6370.10714616115657301No Hit
CTCCTATGATGGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGA6370.10714616115657301No Hit
GTTTAAGGGGTTGGCTAGGGTATAATTGTCTGGGTCGCCTAGGAGGTCTG6330.1064733438180702No Hit
ATATTATGCTTTGTTGTTTGGATATATGGAGGATGGGGATTATTGCTAGG6300.10596873081419307No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG6290.10580052647956738No Hit
GTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAATGT6270.10546411781031596No Hit
CTTCTACTATTAGGACTTTTCGCTTCGAAGCGAAGGCTTCTCAAATCATG6270.10546411781031596No Hit
CATCTACAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCA6260.10529591347569027No Hit
CCTCTATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAA6250.10512770914106458No Hit
GTAGTATAGTGATGCCAGCAGCTAGGACTGGGAGAGATAGGAGAAGTAGG6150.10344566579480753No Hit
ATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTC6140.10327746146018182No Hit
TGATAGGACATAGTGGAAGTGAGCTACAACGTAGTACGTGTCGTGTAGTA6080.10226823545242761No Hit
CCGTAGACCTAACCATCTTCTCCTTACACCTAGCAGGTGTCTCCTCTATC6070.10210003111780191No Hit
GTCCCAATGTATGGGATGGCGGATAGTAAGTTTGTAATTACTGTGGCCCC6030.10142721377929909No Hit
CTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAG6020.10125900944467339No Hit
CATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTTTT6010.1010908051100477No Hit
CTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCT6010.1010908051100477No Hit
GTCTACGTCTATTCCTACTGTAAATATATGGTGTGCTCACACGATAAACC5950.10008157910229346No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)