FastQCFastQC Report
Tue 10 May 2022
EGAF00003635973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003635973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451190
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC9020.19991577827522772No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG8370.18550943061681333No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7360.16312418271681553Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC7330.16245927436335025No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT7170.15891309647820206No Hit
GTATAGCGAAAGGGAAGCCGGTCAATATTCCGGCGCCTGGATTGGGGTTT6240.13830093752077838No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA6150.13630621246038255No Hit
GTGTAACAACTCACCTGCCGAATGTACTAGCCCTGAAAATGGATGGCGCT6060.13431148739998672No Hit
GTATAATACCTTTATGGGGACCGAGGTTCGCGCTTCGGCAAGGATGCTGG5740.12721913162969037No Hit
TCGCTATACGGTGCACAAGTGCACACTTTGAAACGGAACTTCCCTATAGC5640.12500277045147276No Hit
GGGTAGAAACGATGCCCTGAGGAGTAGGCGGCCGTGGGGGTCAGTGACGA5530.1225647731554334No Hit
GTATTTATTGTCACTACCTCCCCGAATCGGGATTGGGTAATTTGCGCGCC5480.1214565925663246No Hit
GCTAAGGACTGTGTAACAACTCACCTGCCGAATGTACTAGCCCTGAAAAT5150.11414260067820652No Hit
GTATTATACAATAGGCTATAACACTCCCGAGGGAGCCACATTCCTACTGC5020.11126133114652363No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT5020.11126133114652363No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG4930.1092666060861278No Hit
ATATTGCCTTCATCCATTCTCAAACTTTAAATATGTAAGAAGCCCTTGTT4710.10439061149404907No Hit
CTCAATAAAGAGCATGCGGCTCCCCAGAGGGAAAGGCCCGGCCGAACCAG4700.1041689753762273No Hit
GTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCAAGATGT4680.1037257031405838No Hit
GTACATGGGTACCTGGTTGATTCTGCCAGTAGTCATATGCTTGTCTCAAA4530.10040116137325739No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)