FastQCFastQC Report
Tue 10 May 2022
EGAF00003635974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003635974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451190
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC9350.20722977016334582No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC7430.16467563554156786No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7320.1622376382455285Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT7060.1564750991821627No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG7000.15514528247523215No Hit
GTATAGCGAAAGGGAAGCCGGTCAATATTCCGGCGCCTGGATTGGGGTTT6070.13453312351780847No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA5990.1327600345752344No Hit
GTATAATACCTTTATGGGGACCGAGGTTCGCGCTTCGGCAAGGATGCTGG5680.12588931492275982No Hit
GTATTATACAATAGGCTATAACACTCCCGAGGGAGCCACATTCCTACTGC5660.1254460426871163No Hit
GTGTAACAACTCACCTGCCGAATGTACTAGCCCTGAAAATGGATGGCGCT5640.12500277045147276No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT5500.12189986480196813No Hit
TCGCTATACGGTGCACAAGTGCACACTTTGAAACGGAACTTCCCTATAGC5330.11813205079899822No Hit
GTATTTATTGTCACTACCTCCCCGAATCGGGATTGGGTAATTTGCGCGCC5290.11724550632771116No Hit
CAATAAAGAGCATGCGGCTCCCCAGAGGGAAAGGCCCGGCCGAACCAGTG5260.11658059797424589No Hit
GCTAAGGACTGTGTAACAACTCACCTGCCGAATGTACTAGCCCTGAAAAT5160.11436423679602828No Hit
CTTGTGCACCGTATAGCGAAAGGGAAGCCGGTCAATATTCCGGCGCCTGG4950.10970987832177133No Hit
CTCAATAAAGAGCATGCGGCTCCCCAGAGGGAAAGGCCCGGCCGAACCAG4780.10594206431880139No Hit
GGGTAGAAACGATGCCCTGAGGAGTAGGCGGCCGTGGGGGTCAGTGACGA4680.1037257031405838No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG4640.10283915866929674No Hit
GTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCAAGATGT4610.10217425031583147No Hit
GTGCACAAGTGCACACTTTGAAACGGAACTTCCCTATAGCTTAGGATCGG4580.10150934196236619No Hit
GTATTTATTAGATTAAAAACCAATGCCCTTCGGGGCTCTTTGGTGATTCA4520.10017952525543564No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)