FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003636021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003636021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA23.508771929824561Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGTGTGTGTGTGTTTGTATTTTTTCAGTTTCATTTGGTTTTGCTCTGAT23.508771929824561No Hit
GTTCAAGTTATTCTCATGCCTCAGCCTCCCCAGTAGCCGAGATTACAGAT23.508771929824561No Hit
GTCGGAGGTGTTCCTGAATCGGTGGCATTAATCTAGAGGCCATGTTCTGC23.508771929824561No Hit
GAATCGAATGGAATCATCATCAAATGGAATCTGATGGAATCATTGAACAG23.508771929824561No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG23.508771929824561Clontech Universal Primer Mix Long (96% over 26bp)
CCATGGTGCGGCGCCCGCCGACCATCAACGGCAAGTTCGTGAGCATCAAC23.508771929824561No Hit
CCCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTTGGTTTTGATCTGAT23.508771929824561No Hit
CAGTAATTCCTGGAGGAATAGGTACCAAGAAAAAAACGAACCTTTGGGTT23.508771929824561No Hit
CTTTAAATATAAGGCGTGGTAGAATTACTGGCACTCCAATGATTCCATAT23.508771929824561No Hit
AGGCATCACTTCCCCAACGGCCAATATTCTGCAATTTCTGGTCGCGGGTG23.508771929824561No Hit
ATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCATGTC11.7543859649122806No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCGAGTACATGGGAAGC11.7543859649122806Clontech SMART CDS Primer II A (96% over 26bp)
CAGTGGCACATGTCTGTAGTCTCAGCTATTTGGGAGGCTGAGGCAGGGGG11.7543859649122806No Hit
GGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCT11.7543859649122806No Hit
GTATGAGTCAGTAAGGACTGGAGAACAGCACTTCTCTAGGCACCTGGTGG11.7543859649122806No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTGTTTTTTTTTT11.7543859649122806No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACATTTCAAACAAC11.7543859649122806No Hit
GTGAAGGAAACCTCTCTCGCAACTCAGAAATAATTAAATCTTCCACAAGG11.7543859649122806No Hit
AGGTAGTAGTGGCACTTGTCGGTGCAGGCGCCGCACTTGACGCACGAGTC11.7543859649122806No Hit
GTCTTCTTCTCCCCTATTGACGTGTCGTCCAGGGCCTGTCTCTTATACAC11.7543859649122806No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTCTTATACACATCT11.7543859649122806Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTCTTATAC11.7543859649122806Clontech Universal Primer Mix Long (96% over 26bp)
GAGTTAGGAAGGAGTCCTTCCTCCTCAATTTTTTGGAATAGTTTTAGTAG11.7543859649122806No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG11.7543859649122806Clontech Universal Primer Mix Long (96% over 26bp)
AAATTGCTCAGGGTAGGTTTGGAGAGTGAACACCAGTTTAGTTCAGGGAA11.7543859649122806No Hit
GTACTTTTTTTTTTTTTTTTTGCGTTGATACCACTGCTTCCCATGTACCT11.7543859649122806Clontech Universal Primer Mix Long (95% over 21bp)
GCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACT11.7543859649122806No Hit
TGGGAAGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAGT11.7543859649122806No Hit
AGTCTACTTAGCCAGAAAGTTCCCAGGGTCCAGAATGTTGACACATGGTA11.7543859649122806No Hit
GATTTAATTCTGCATTTAATTGATTCTTCAAATGAGGATTATGCGGGACA11.7543859649122806No Hit
TCTGAGGGCAGTTGTTTACAATTCAAAAAGAGGAGCATCACCAGACACCA11.7543859649122806No Hit
GTACATGGGAATCAGTGGTATCAACAGAAAAAAAAAAAAAAAAAAAAAAA11.7543859649122806No Hit
GCACTACACATGCTAGCCGCTGGGGAGATTCTGTCTCTTATACACATCTC11.7543859649122806No Hit
GCCCTCAACTCCCTCCATGAAAGATGGAGAATTAACCTTGGGCTTCCTGT11.7543859649122806No Hit
GAGATACAACCCTATGTGATTGCATTTTCCAGTCAATTGTTGGGTTTCTT11.7543859649122806No Hit
CCCCGTCTCTACTAAAAATACAAAAATTAGCCAGACGTGGTGGTGGGCAC11.7543859649122806No Hit
GATTTATGGATTCAATGCAATTGCTATCAAACTACCAATGTCTTTCTTCA11.7543859649122806No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTGGGGTTTTAACCCA11.7543859649122806No Hit
TCAAAGGAATGATCTGCAGAGCAAGCAGCTCTGCCACCTTGGAAAATGAT11.7543859649122806No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAACTACTATGCGTTTATACCAA11.7543859649122806No Hit
CGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACACTGCGTTGATA11.7543859649122806No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCAC11.7543859649122806Clontech SMART CDS Primer II A (100% over 21bp)
GGTGAGTCTATAAATTTTTGTCATAAATGCCACTCGGTTTGTGGCATTTT11.7543859649122806No Hit
ATAGGAGAGATTGCAATTACAAGTGAAATTTCAGTATATTTTTCAGTAGA11.7543859649122806No Hit
GGCCCGACCGGCAGCTTGAGCACGTCCATGCGCGACTGCGCGGCACGCGC11.7543859649122806No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)